We consider multipartite quantum state discrimination and show that the minimum-error discrimination by separable measurements is closely related to the concept of entanglement witness. Based on the properties of entanglement witness, we establish some necessary and/or sufficient conditions on minimum-error discrimination by separable measurements. We also provide some conditions on the upper bound of the maximum success probability over all possible separable measurements. Our results are illustrated by examples of multidimensional multipartite quantum states. Finally, we provide a systematic way in terms of EW to construct multipartite quantum state ensembles showing nonlocality in state discrimination.