Nonlinear photochemistry and 3D microfabrication with Q-switched Nd:YAG microchip lasers

Baldeck, Patrice L.; Scheul, Teodora; Bouriau, Michel; Stéphan, Olivier; Malval, Jean Pierre; Lin, Chih Lang; Lin, Chin Te; Tseng, Chang Li; Huang, Cheng; Chung, Tien Tung

doi:10.1117/12.893642

Cited by 1 publication

(1 citation statement)

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“…This allows crosslinking to occur at any specified point within a solution [2]. Ultrafast pulsed lasers are used to deliver the high optical intensity needed to initiate these reactions [3][4][5]. Movement of the focal point either by directly translating the stage or by raster-scanning of the input laser beam provides means for controlling the crosslinked geometry [6].…”

Section: Introductionmentioning

confidence: 99%

Multiphoton crosslinking for biocompatible 3D printing of type I collagen

2015

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Multiphoton fabrication is a powerful technique for three-dimensional (3D) printing of structures at the microscale. Many polymers and proteins have been successfully structured and patterned using this method. Type I collagen comprises a large part of the extracellular matrix for most tissue types and is a widely used cellular scaffold material for tissue engineering. Current methods for creating collagen tissue scaffolds do not allow control of local geometry on a cellular scale. This means the environment experienced by cells may be made up of the native material but unrelated to native cellular-scale structure. In this study, we present a novel method to allow multiphoton crosslinking of type I collagen with flavin mononucleotide photosensitizer. The method detailed allows full 3D printing of crosslinked structures made from unmodified type I collagen and uses only demonstrated biocompatible materials. Resolution of 1 μm for both standing lines and high-aspect ratio gaps between structures is demonstrated and complex 3D structures are fabricated. This study demonstrates a means for 3D printing with one of the most widely used tissue scaffold materials. High-resolution, 3D control of the fabrication of collagen scaffolds will facilitate higher fidelity recreation of the native extracellular environment for engineered tissues.

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