Nonlinear Local Deformations of Erythrocyte Membranes: Normal Erythrocytes (Part 1)

Черныш, А. М.; Козлова, Е. К.; Мороз, В. В.; Сергунова, В. А.; Гудкова, О. Е.; Козлов, А. П.; Манченко, Е. А.

doi:10.15360/1813-9779-2017-5-58-68

Cited by 3 publications

(3 citation statements)

References 20 publications

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“…In this work, we estimated the membrane's ability to bend into the cell, by atomic force spectroscopy [23] with large radius probes ( R = 150 nm). In all experiments, only native cells were used.…”

Section: Discussionmentioning

confidence: 99%

“…The numbers of scan points were 512 and 1024. Measurements of local stiffness of RBC membranes were performed by ASF on the vertical displacement of a piezoscanner, where the sample was placed [14, 23]. To measure the deformation of the membrane, the type SD-R150-T3L450B-10 (Nanosensors, Switzerland) cantilever was used.…”

Section: Methodsmentioning

confidence: 99%

“…Cantilever calibration on highly rigid material (e.g., glass) must be carried out before and after each series of measurements [14, 23]. The empirical force curve is the dependence of photodiode deflection current I on the magnitude of the vertical displacement Z of the piezoscanner, I ( Z ).…”

Section: Methodsmentioning

confidence: 99%

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Nonlinear Biomechanical Characteristics of Deep Deformation of Native RBC Membranes in Normal State and under Modifier Action

et al. 2018

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The ability of membranes of native human red blood cells (RBCs) to bend into the cell to a depth comparable in size with physiological deformations was evaluated. For this, the methods of atomic force microscopy and atomic force spectroscopy were used. Nonlinear patterns of deep deformation (up to 600 nm) of RBC membranes were studied in normal state and under the action of modifiers: fixator (glutaraldehyde), natural oxidant (hemin), and exogenous intoxicator (zinc ions), in vitro. The experimental dependences of membrane bending for control RBC (normal) were approximated by the Hertz model to a depth up to 600 nm. The glutaraldehyde fixator and modifiers increased the absolute value of Young's modulus of membranes and changed the experimental dependences of probe indentation into the cells. Up to some depth hHz, the force curves were approximated by the Hertz model, and for deeper indentations h > hHz, the degree of the polynomial function was changed, the membrane stiffness increased, and the pattern of indentation became another and did not obey the Hertz model. Quantitative characteristics of nonlinear experimental dependences were calculated for deep bending of RBC membranes by approximating them by the degree polynomial function.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Nonlinear Biomechanical Characteristics of Deep Deformation of Native RBC Membranes in Normal State and under Modifier Action

et al. 2018

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Homogeneous Deformation of Native Erythrocytes During Long-Term Storage

Манченко

Козлова

Сергунова

et al. 2019

Obŝaâ reanimatologiâ

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Purpose of the study — to evaluate biomechanical regularities of deep deformation of native erythrocytes’ membranes during long-term (up to 32 days) storage of erythrocyte suspension.Materials and methods. The method for addressing the said problem was atomic-force spectroscopy. The measured value was hHz comprizing the depth to which the probe immersion process was described by interaction with a homogeneous medium. Empirical and theoretical dependence of the interaction force F (nN) on the probe immersion depth h (nm) — F (h) were obtained. Bar charts of relative frequency density of Young’s modulus E were built.Results. Modulus E changed from 9.3±3.2 kPa — for 3 days of storage, to 22.7±8.7 kPa — for 32 days. Coefficients of skewness were 0.52±0.04 (for day 3) and 0.82±0.09 (for day 32 d), hHz value remaining constant.Conclusion. Progressively as erythrocyte suspension was stored, erythrocyte membranes to the depth of 700 nm deflected homogeneously in spite of 2.4-fold increase of Young’s modulus.

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Ultrastructural characteristics of erythrocytes and their relationship with oxygen transport in patients with ischemic heart disease after coronary artery bypass grafting with artificial circulation

Sergeev

Shmelev

Гельцер

et al. 2021

Bûlletenʹ fiziologii i patologii dyhaniâ

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Aim. Evaluation of nanostructural and micromechanical characteristics of erythrocytes and their relationship with the parameters of oxygen transport in patients with ischemic heart disease (IHD) after coronary artery bypass grafting (CABG) under artificial circulation (AC).Materials and methods. The study involved 30 men with IHD aged 53 to 67 years with a median of 63 years, who underwent planned CABG with AC at the clinic of the Far Eastern Federal University. Blood sampling from patients was carried out before the AC and one day after it from a venous catheter located in the right atrium. Erythrocytes were scanned using a Bioscope Catalyst atomic force microscope (AFM) (Bruker, USA) in combination with an Axiovert 200 inverted microscope (Zeiss, Germany). The micromechanical properties of cell membranes were investigated with the PFQNM-LC-A-CAL cantilevers. The following were recorded: the diameter of erythrocytes, the height of their side, area, volume, membrane rigidity, strength of its adhesion, Young's modulus, elastic deformation. To assess oxygen transport, the oxygen delivery index (DO2I), consumption (VO2I), and extraction ratio (O2ER) were calculated.Results. In one day after CABG, a decrease in DO2I, VO2I, and O2ER was recorded by 14%, 33%, and 16%, respectively, which indicated a limitation in the gas transport function of the blood. Analysis of the AFM characteristics of erythrocytes showed an increase in their area by 35%, in diameter by 6%, in volume by 19%, and the height of the side by 5 times. At the same time, multidirectional changes in the micromechanical properties of erythrocyte membranes were noted: Young's modulus and stiffness decreased by 3.2 and 2 times, respectively; the adhesion force increased by 2.7 times, and the elastic deformation – by 2.2 times. Correlation analysis showed the presence of reliable relationships between indicators of oxygen transport and biomechanical parameters of erythrocyte membranes.Conclusion. A comprehensive analysis of the nanostructural parameters of erythrocytes and oxygen transport expands the understanding of the pathophysiological mechanisms of postperfusion disorders in patients with coronary artery disease after CABG under conditions of cardiopulmonary bypass.

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Nonlinear Local Deformations of Erythrocyte Membranes: Normal Erythrocytes (Part 1)

Cited by 3 publications

References 20 publications

Nonlinear Biomechanical Characteristics of Deep Deformation of Native RBC Membranes in Normal State and under Modifier Action

Nonlinear Biomechanical Characteristics of Deep Deformation of Native RBC Membranes in Normal State and under Modifier Action

Homogeneous Deformation of Native Erythrocytes During Long-Term Storage

Ultrastructural characteristics of erythrocytes and their relationship with oxygen transport in patients with ischemic heart disease after coronary artery bypass grafting with artificial circulation

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