Nonisotopic In Situ Hybridization: Immunocytochemical Detection of Specific Repetitive Sequences on Chromosomes and Interphase Nuclei

Ja, Crolla

doi:10.1385/0-89603-204-3:431

Cited by 2 publications

(1 citation statement)

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“…In situ hybridization I n situ hybridization using biotinylation and immunochemical detection methods, described in detail elsewhere (Crolla et al 1989;Crolla, 1990), was carried out on nine cases with a ring chromosome using either previously unstained or archived slides. All cases were hybridized with an alphoid X centromere-specific probe, DXZ2 (pSV2X5; Wolfe et al 1985), and the two cases negative for DXZS were also hybridized with a Yp-specific probe, DYZ5 (Y190; Muller et al 1987), and an alphoid Y centromere-specific probe, DYZ3 (Y84; Wolfe et al 1985).…”

Section: Cytogeneticsmentioning

confidence: 99%

A cytogenetic and molecular reappraisal of a series of patients with Turner's syndrome

Jacobs

Betts

Cockwell

et al. 1990

Annals of Human Genetics

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The results of a cytogenetic and molecular reinvestigation of a series of 52 patients with Turner's syndrome are reported. No evidence of Y chromosome material was found among the patients with a 45,X constitution but two patients were found to have a cell line with a r(Y) chromosome which was previously thought to be a r(X). The parental origin of the single X in the 45,X patients was maternal in 69% and paternal in 31%, a similar ratio to that seen among spontaneously aborted 45,X conceptuses. This suggests that X-chromosome imprinting is not responsible for the two grossly different phenotypes associated with a 45,X chromosome constitution. Approximately half of the structurally abnormal X chromosomes were maternal in origin and half paternal. This observation is consistent with either a meiotic or post-zygotic mitotic origin and at variance with the predominantly paternal origin reported for autosome structural abnormalities.

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Section: Cytogeneticsmentioning

confidence: 99%

A cytogenetic and molecular reappraisal of a series of patients with Turner's syndrome

Jacobs

Betts

Cockwell

et al. 1990

Annals of Human Genetics

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Molecular cytogenetic characterization of three familial cases of satellited Y chromosomes

Wilkinson

Crolla

1993

Hum Genet

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Three families in which a satellited Y chromosome (Yqs) was segregating without apparent phenotypic effect were re-investigated with non-isotopic in situ hybridization methods. Active nucleolus organizer regions were seen in the distal long arm region of all Yqs chromosomes studied and in situ hybridization with the probe D15Z1 showed that, in all three families, the Yqs was the result of a 15p;Yq translocation. In one case, an additional focus of D15Z1 hybridization was seen on 21p.

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Nonisotopic In Situ Hybridization: Immunocytochemical Detection of Specific Repetitive Sequences on Chromosomes and Interphase Nuclei

Cited by 2 publications

References 10 publications

A cytogenetic and molecular reappraisal of a series of patients with Turner's syndrome

A cytogenetic and molecular reappraisal of a series of patients with Turner's syndrome

Molecular cytogenetic characterization of three familial cases of satellited Y chromosomes

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