Nonionizing photoacoustic cystography in vivo

Kim, Chulhong; Jeon, Mansik; Wang, Lihong V.

doi:10.1364/ol.36.003599

Cited by 59 publications

(30 citation statements)

References 19 publications

(23 reference statements)

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“…These PA experimental results revealed that N-CNDs could be removed from the body by renal clearance after subcutaneous injection. The results showed that N-CNDs can contribute to the diagnosis of urinary tract diseases, including leaks and fistulas 61.…”

Section: Resultsmentioning

confidence: 96%

Biodegradable Nitrogen-Doped Carbon Nanodots for Non-Invasive Photoacoustic Imaging and Photothermal Therapy

Lee¹,

Kwon²,

Beack³

et al. 2016

Theranostics

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139

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Multifunctional nanoparticles have been widely investigated for biomedical applications, such as imaging, therapy, and drug delivery. Especially, photoactive nanoparticles have received great attention as theranostic agents because of their heat-generating abilities after exposure to laser irradiation. However, photostability and safety issues have been the technical hurdles for further clinical applications. Here, we designed nitrogen (N)-doped carbon nanodots (N-CNDs) that have strong absorption in the near-infrared region, high photostability, and excellent biodegradability. Optimized N-CNDs can be utilized not only as a new photoacoustic (PA) imaging agent but also as a superior photothermal therapy (PTT) agent in vivo because of their strong optical absorption at a specific wavelength. We used N-CNDs to perform in vivo/ex vivo noninvasive PA imaging of sentinel lymph nodes via local delivery and performed PTT for cancer ablation therapy. Finally, biodegradation and renal clearance were confirmed by performing whole-body PA monitoring and a degradation test.

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Section: Resultsmentioning

confidence: 96%

Biodegradable Nitrogen-Doped Carbon Nanodots for Non-Invasive Photoacoustic Imaging and Photothermal Therapy

Lee¹,

Kwon²,

Beack³

et al. 2016

Theranostics

Self Cite

139

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“…Tissue samples were characterized ex vivo using a reflection-mode photoacoustic (PA) system based on a novel design [42]. Briefly, laser pulses were generated from a wavelength-tunable laser (Surelite™ OPO PLUS; Continuum, CA, USA) at a wavelength of 815 nm with a repetition rate of 10 Hz and a pulse duration of 5 ns, pumped by a Q-switched Neodynium:YAG laser (SLII-10; Continuum; 532 nm).…”

Section: Methodsmentioning

confidence: 99%

Synergistic Effects of Cisplatin Chemotherapy and Gold Nanorod-Mediated Hyperthermia on Ovarian Cancer Cells and Tumors

Mehtala

Torregrosa-Allen²,

Elzey

et al. 2014

Nanomedicine (Lond.)

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Aim The synergistic effects of gold nanorod (GNR)-mediated mild hyperthermia (MHT; 42–43°C) and cisplatin (CP) activity was evaluated against chemoresistant SKOV3 cells in vitro and with a tumor xenograft model. Materials & methods In vitro studies were performed using CP at cytostatic concentrations (5 μM) and polyethylene glycol-stabilized GNRs, using near-infrared laser excitation for MHT. Results The amount of polyethylene glycol-GNRs used for environmental MHT was 1 μg/ml, several times lower than the loadings used in tumor tissue ablation. GNR-mediated MHT increased CP-mediated cytotoxicity by 80%, relative to the projected additive effect, and flow cytometry analysis suggested MHT also enhanced CP-induced apoptosis. In a pilot in vivo study, systemically administered polyethylene glycol-GNRs generated sufficient levels of MHT to enhance CP-induced reductions in tumor volume, despite their heterogeneous distribution in tumor tissue. Conclusion These studies imply that effective chemotherapies can be developed in combination with low loadings of nanoparticles for localized MHT.

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“…By using only a laser pulse energy of less than 1 mJ/cm 2 (1/20 of the safety limit), our current imaging system could map the methylene-blue-filled-rat-bladder at the depth of beyond 1 cm in biological tissues in vivo. 17 As we above mentioned, the imaging depth of PAT can reach up to ~8 cm in tissues with aid of methylene blue. 9 After all in vivo imaging experiments, we excised two major organs, bladder and kidney to investigate biodistribution.…”

Section: In Vivo and Ex Vivo Imaging Proceduresmentioning

confidence: 93%

Photoacoustic Cystography

Jeon

Kim²,

Kim³

2013

JoVE

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Conventional pediatric cystography, which is based on diagnostic X-ray using a radio-opaque dye, suffers from the use of harmful ionizing radiation. The risk of bladder cancers in children due to radiation exposure is more significant than many other cancers. Here we demonstrate the feasibility of nonionizing and noninvasive photoacoustic (PA) imaging of urinary bladders, referred to as photoacoustic cystography (PAC), using near-infrared (NIR) optical absorbents (i.e. methylene blue, plasmonic gold nanostructures, or single walled carbon nanotubes) as an optical-turbid tracer. We have successfully imaged a rat bladder filled with the optical absorbing agents using a dark-field confocal PAC system. After transurethral injection of the contrast agents, the rat's bladders were photoacoustically visualized by achieving significant PA signal enhancement. The accumulation was validated by spectroscopic PA imaging. Further, by using only a laser pulse energy of less than 1 mJ/cm 2 (1/20 of the safety limit), our current imaging system could map the methylene-blue-filled-rat-bladder at the depth of beyond 1 cm in biological tissues in vivo. Both in vivo and ex vivo PA imaging results validate that the contrast agents were naturally excreted via urination. Thus, there is no concern regarding long-term toxic agent accumulation, which will facilitate clinical translation. Video LinkThe video component of this article can be found at

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Nonionizing photoacoustic cystography in vivo

Cited by 59 publications

References 19 publications

Biodegradable Nitrogen-Doped Carbon Nanodots for Non-Invasive Photoacoustic Imaging and Photothermal Therapy

Biodegradable Nitrogen-Doped Carbon Nanodots for Non-Invasive Photoacoustic Imaging and Photothermal Therapy

Synergistic Effects of Cisplatin Chemotherapy and Gold Nanorod-Mediated Hyperthermia on Ovarian Cancer Cells and Tumors

Photoacoustic Cystography

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