Nonionic surfactants enhanced enzymatic hydrolysis of cellulose by reducing cellulase deactivation caused by shear force and air-liquid interface

Li, Hongming; Zeng, Meijun; Hu, Qiaoyan; Cai, Cheng; Lin, Xuliang; Qiu, Xueqing; Yang, Dongjie; Pang, Yongqiang

doi:10.1016/j.biortech.2017.07.066

Cited by 84 publications

(57 citation statements)

References 37 publications

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“…However, this typically comes with an increase in enzyme loads, and hence, the overall economic value is low. Low substrate loadings (<5% solids, w/w) is favored in biomass biological conversion technologies to ensure good wetting, enzyme accessibility, and to minimize any inhibitory effect of the hydrolysis products [11,12,19]; still, the enzyme load is recalculated based on the biomass added to the system. In this study, initial batch scale tests with hay and commercial enzyme preparation demonstrated that the amount of released reducing sugar tends to decrease (0-30%) with each biomass wt%.…”

Section: Impact Of Solid Loading On Hydrolysis Efficiencymentioning

confidence: 99%

“…As shown, a minimum wheat straw loading of~20 wt% is required in order to reach the desired final ethanol concentration [7] of 4 wt% that is typically necessary for an efficient distillation step [10]. Solid loadings of up to 25 wt% have been demonstrated for corncob [11] and cellulose [12]. Nevertheless, the main drawbacks of this approach are the need for specific reactor systems and low-liquid pre-treatment/hydrolysis conditions.…”

Section: Introductionmentioning

confidence: 99%

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The Impact of Substrate–Enzyme Proportion for Efficient Hydrolysis of Hay

Mežule¹,

Bērziņa²,

Strods³

2019

Energies

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Fuel alcohol production yields can be influenced by lignocellulosic biomass loading. High solid loadings (>20 wt%) are suggested to have the potential to produce more products. However, most often, low substrate loadings (<5% solids, w/w) are used to ensure good wetting and enzyme accessibility, and to minimize any inhibitory effect on the hydrolysis products. Here, we analyzed the effect of substrate loading on the enzymatic hydrolysis of hay with non-commercial enzyme products obtained from white-rot fungi. A significant negative effect on hydrolysis was observed when 10 wt% hay loading was used with the commercial enzyme, however, non-commercial enzyme products from white-rot fungi had no impact on hydrolysis in biomass loading rates from 1 to 10 wt%. Moreover, it was estimated that enzymes extracted from white-rot fungi could be used at a concentration of 0.2 FPU/mL at a biomass loading from 1–10 wt%, resulting in 0.17–0.24 g of released reducing carbohydrates per gram of biomass. Higher concentrations did not result in any significant conversion increase. A mixing impact was only observed in test runs at a substrate loading of 10 wt%. The apparently positive features of the non-commercial enzyme mixes give rise to their future use. The combination and upgrade of existing technologies, e.g., efficient pre-treatment, membrane purification, and concentration and efficient product recovery, should result in even higher conversion yields.

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Section: Impact Of Solid Loading On Hydrolysis Efficiencymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The Impact of Substrate–Enzyme Proportion for Efficient Hydrolysis of Hay

Mežule¹,

Bērziņa²,

Strods³

2019

Energies

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“…[133][134][135][136] Moreover, mild agitation and exposure to air-liquid interface could inhibit the activity of the enzyme. 137 Lou and his co-workers have demonstrated the enhancement of enzymatic hydrolysis of cellulose using non-ionic surfactants (PEG4600) to reduce this deactivation, wherein the surfactant competes with cellulase in the air-liquid interface shielding it from exposure to reduce deactivation of enzymatic activity. PEG4600 also forms a hydration shell around cellulase through hydrogen bonding to lubricate the effect of shear force on the enzyme.…”

Section: Enzymatic Treatmentmentioning

confidence: 99%

Recent advances in 3D printing of nanocellulose: structure, preparation, and application prospects

2021

Nanoscale Adv.

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“…Therefore, the MBGS method has unique advantages of improving the chemical stability of immobilized enzymes and maintaining high catalytic activity (Itabaiana et al 2014;Pavlidis et al 2010). In addition, surfactants play an important role in the preparation of nanomaterials (Helle et al 2010;Lou et al 2017;Seo et al 2011b). For the preparation of nanocarriers, forming the nano-template by micelles and emulsions of surfactants is a common method that can greatly reduce the surface tension of the solvent and change the interface composition and structure (Bao et al 2019; Carter and Puig-Sellart 2016;…”

Section: Introductionmentioning

confidence: 99%

Function Of Surfactants In Immobilization of Cellulase And Multiphase Hydrolysis: A Review

Wang

De-yi

Wang

et al. 2021

Preprint

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Surfactants, especially non-ionic surfactants, play an important role in the preparation of nanocarriers and can also promote the enzymatic hydrolysis of lignocellulose. A broad overview of the current status of surfactants on the immobilization of cellulase is provided in this review. In addition, the restricting factors in cellulase immobilization in the complex multiphase hydrolysis system are discussed, including the carrier structure characteristics, solid-solid contact obstacles, external diffusion resistance, limited recycling frequency, and invalid combination of enzyme active centers. Furthermore, promising prospects of cellulase-oriented immobilization are proposed, including the hydrophilic-hydrophobic interaction of surfactants and cellulase in the oil-water reaction system, the reversed micelle system of surfactants, and the possible oriented immobilization mechanism.

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Nonionic surfactants enhanced enzymatic hydrolysis of cellulose by reducing cellulase deactivation caused by shear force and air-liquid interface

Cited by 84 publications

References 37 publications

The Impact of Substrate–Enzyme Proportion for Efficient Hydrolysis of Hay

The Impact of Substrate–Enzyme Proportion for Efficient Hydrolysis of Hay

Recent advances in 3D printing of nanocellulose: structure, preparation, and application prospects

Function Of Surfactants In Immobilization of Cellulase And Multiphase Hydrolysis: A Review

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