Nonionic Block Polymer Surfactants as Adjuvants in Vaccines

Zigterman, G. J. W. J.; Verheul, A. F. M.; Snippe, H.

doi:10.1007/978-1-4615-3848-6_7

Cited by 1 publication

(1 citation statement)

References 18 publications

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“…Especially the immune response against BSA conjugates, which are weaker immunogens than KLH conjugates (29,39), can be stimulated adequately by NBPs. In a separate study in which the adjuvant potencies of different NBPs were compared, NBPs with hydrophilic POE ends manifested greater adjuvant activity than NBPs with hydrophobic POP ends (36). Therefore, NBPs with hydrophilic POE ends were used in this study on the modulation of anti-HS-protein responses.…”

Section: Discussionmentioning

confidence: 99%

Nonionic block polymer surfactants modulate the humoral immune response against Streptococcus pneumoniae-derived hexasaccharide-protein conjugates

et al. 1989

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Nonionic block polymer surfactants (NBPs) were tested for the capacity to stimulate the antibody response against hexasaccharide (HS), derived from Streptococcus pneumoniae type 3 capsular polysaccharide (S3PS), which was conjugated to proteins. The immune response was evaluated in the (CBA/N x BALB/c)F1 progeny, in which female mice are phenotypically normal whereas male mice carry an X-chromosome-linked immunodeficiency. NBPs L101, L121, 1101, and 1501 were able to increase anti-HS immunoglobulin M (IgM) and IgG levels in both normal and X-chromosome-linked immunodeficient mice (with up to 74-fold stimulation of antibody titers). Distribution of S3PS-specific antibodies over the various IgG isotypes was restricted after immunization with either HS-bovine serum albumin or HS-keyhole limpet hemocyanin (HS-KLH). Addition of NBPs (in particular 1501) resulted in a more diverse immune response with either antigen as judged by isotype distribution. Isoelectric focusing of individual sera and subsequent detection of S3PS-binding antibodies in these sera by immunochemical staining revealed a restricted number of different spectrotypes in the course of the immune response. Upon immunization of mice with HS-KLH, spectra of secreted antibodies were slightly more complex and more densely stained than after immunization with HS-bovine serum albumin. Furthermore, NBPs 1101 and 1501 appeared to be able to stimulate the secretion of antibodies, which were secreted only in small amounts without the use of NBPs. Different explanations for increased spectrotype diversity after immunization with KLH as the carrier and after administration of NBPs as the adjuvant are discussed.

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Section: Discussionmentioning

confidence: 99%