Noninvasive Ultrasonic Measurement of Regional and Local Pulse-Wave Velocity in Mice

Williams, Ronald J.; Needles, A.; Chérin, Emmanuel; Zhou, Yuqing; Henkelman, R. Mark; Adamson, S. Lee; Foster, F. Stuart

doi:10.1016/j.ultrasmedbio.2007.03.012

Cited by 75 publications

(59 citation statements)

References 19 publications

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“…Depilatory cream was used to remove fur from the throat, and ultrasound gel was used. Carotid pulsed‐wave Doppler ultrasound velocity measurements were performed as an estimation of arterial stiffness 25. Mean velocity, end‐diastolic velocity, and peak systolic velocity were determined, and the resistance index was calculated as follows: resistance index=(peak systolic velocity−end‐diastolic velocity)/peak systolic velocity.…”

Section: Methodsmentioning

confidence: 99%

Nonmuscle Myosin Light Chain Kinase: A Key Player in Intermittent Hypoxia‐Induced Vascular Alterations

Arnaud

Bouyon

Recoquillon

et al. 2018

JAHA

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BackgroundObstructive sleep apnea is characterized by repetitive pharyngeal collapses during sleep, leading to intermittent hypoxia (IH), the main contributor of obstructive sleep apnea–related cardiovascular morbidity. In patients and rodents with obstructive sleep apnea exposed to IH, vascular inflammation and remodeling, endothelial dysfunction, and circulating inflammatory markers are linked with IH severity. The nonmuscle myosin light chain kinase (nmMLCK) isoform contributes to vascular inflammation and oxidative stress in different cardiovascular and inflammatory diseases. Thus, in the present study, we hypothesized that nmMLCK plays a key role in the IH‐induced vascular dysfunctions and inflammatory remodeling.Methods and ResultsTwelve‐week‐old nmMLCK +/+ or nmMLCK −/− mice were exposed to 14‐day IH or normoxia. IH was associated with functional alterations characterized by an elevation of arterial blood pressure and stiffness and perturbations of NO signaling. IH caused endothelial barrier dysfunction (ie, reduced transendothelial resistance in vitro) and induced vascular oxidative stress associated with an inflammatory remodeling, characterized by an increased intima‐media thickness and an increased expression and activity of inflammatory markers, such as interferon‐γ and nuclear factor‐κB, in the vascular wall. Interestingly, nmMLCK deletion prevented all IH‐induced functional and structural alterations, including the restoration of NO signaling, correction of endothelial barrier integrity, and reduction of both oxidative stress and associated inflammatory response.ConclusionsnmMLCK is a key mechanism in IH‐induced vascular oxidative stress and inflammation and both functional and structural remodeling.

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Section: Methodsmentioning

confidence: 99%

Nonmuscle Myosin Light Chain Kinase: A Key Player in Intermittent Hypoxia‐Induced Vascular Alterations

Arnaud

Bouyon

Recoquillon

et al. 2018

JAHA

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“…A customwritten platform in Matlab, based on the algorithm described by Rabben et al (2002), was used to reconstruct the complete Guo and Kassab, 2003;Guo et al, 2006;Herold et al, 2009;Luo et al, 2009;Reddy et al, 2003;Wagenseil et al, 2005;Wang, 2005;Williams et al, 2007). If local ex vivo pressure-diameter curves or distensibility values were reported, these were converted into local pulse wave velocities (PWV) using the Bramwell-Hill equation.…”

Section: Governing Equationsmentioning

confidence: 99%

A 1D model of the arterial circulation in mice

Aslanidou

Trachet

Reymond

et al. 2016

ALTEX

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SummaryAt a time of growing concern over the ethics of animal experimentation, mouse models are still an indispensable source of insight into the cardiovascular system and its most frequent pathologies. Nevertheless, reference data on the murine cardiovascular anatomy and physiology are lacking. In this work, we developed and validated an in silico, one dimensional model of the murine systemic arterial tree consisting of 85 arterial segments. Detailed aortic dimensions were obtained in vivo from contrast-enhanced micro-computed tomography in 3 male, C57BL/6J anesthetized mice and 3 male ApoE -/-mice, all 12-weeks old. Physiological input data were gathered from a wide range of literature data. The integrated form of the Navier-Stokes equations was solved numerically to yield pressures and flows throughout the arterial network. The resulting model predictions have been validated against invasive pressure waveforms and noninvasive velocity and diameter waveforms that were measured in vivo on an independent set of 47 mice. In conclusion, we present a validated one-dimensional model of the anesthetized murine cardiovascular system that can serve as a versatile tool in the field of preclinical cardiovascular research.

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“…One of the applications for peripheral measurements of blood velocity is the determination of arterial pulse wave velocity (PWV) as an index of arterial stiffness (2,68). Stiffer vessels propagate pressure and velocity waves faster than more compliant vessels.…”

Section: Pulse Wave Velocitymentioning

confidence: 99%

Doppler velocity measurements from large and small arteries of mice

Hartley

Reddy

Madala

et al. 2011

American Journal of Physiology-Heart and Circulatory Physiology

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Hartley CJ, Reddy AK, Madala S, Entman ML, Michael LH, Taffet GE. Doppler velocity measurements from large and small arteries of mice. Am J Physiol Heart Circ Physiol 301: H269 -H278, 2011. First published May 13, 2011; doi:10.1152/ajpheart.00320.2011With the growth of genetic engineering, mice have become increasingly common as models of human diseases, and this has stimulated the development of techniques to assess the murine cardiovascular system. Our group has developed nonimaging and dedicated Doppler techniques for measuring blood velocity in the large and small peripheral arteries of anesthetized mice. We translated technology originally designed for human vessels for use in smaller mouse vessels at higher heart rates by using higher ultrasonic frequencies, smaller transducers, and higher-speed signal processing. With these methods one can measure cardiac filling and ejection velocities, velocity pulse arrival times for determining pulse wave velocity, peripheral blood velocity and vessel wall motion waveforms, jet velocities for the calculation of the pressure drop across stenoses, and left main coronary velocity for the estimation of coronary flow reserve. These noninvasive methods are convenient and easy to apply, but care must be taken in interpreting measurements due to Doppler sample volume size and angle of incidence. Doppler methods have been used to characterize and evaluate numerous cardiovascular phenotypes in mice and have been particularly useful in evaluating the cardiac and vascular remodeling that occur following transverse aortic constriction. Although duplex ultrasonic echo-Doppler instruments are being applied to mice, dedicated Doppler systems are more suitable for some applications. The magnitudes and waveforms of blood velocities from both cardiac and peripheral sites are similar in mice and humans, such that much of what is learned using Doppler technology in mice may be translated back to humans. peripheral vascular flow; pulse wave velocity; coronary flow reserve; transverse aortic constriction; pressure overload hypertrophy THIS REVIEW ARTICLE is part of a collection on Assessing Cardiovascular Function in Mice: New Developments and Methods. Other articles appearing in this collection, as well as a full archive of all Review collections, can be found online at http://ajpheart.physiology.org/.The ability to alter the genotype of the mouse has produced numerous models for studying cardiovascular physiology and pathophysiology and has generated a need to evaluate the changes that occur in mice during phenotypic development and maturation (8). The genetic manipulations can alter the structure, anatomy, pathology, and physiology of cells, organs, or systems in ways that can be subtle and unpredictable and that often change with time (6, 33). The major problems in adapting existing methods for use in mice relate to the small size and high heart rates that place extreme demands on both spatial and temporal resolution (10). We will focus here on noninvasive ultrasonic Doppler methods, w...

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Noninvasive Ultrasonic Measurement of Regional and Local Pulse-Wave Velocity in Mice

Cited by 75 publications

References 19 publications

Nonmuscle Myosin Light Chain Kinase: A Key Player in Intermittent Hypoxia‐Induced Vascular Alterations

Nonmuscle Myosin Light Chain Kinase: A Key Player in Intermittent Hypoxia‐Induced Vascular Alterations

A 1D model of the arterial circulation in mice

Doppler velocity measurements from large and small arteries of mice

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