Noninvasive transdermal delivery of liposomes by weak electric current

Hasan, Mahadi; Khatun, Anowara; Fukuta, Tatsuya; Kogure, Kentaro

doi:10.1016/j.addr.2020.06.016

Cited by 35 publications

(34 citation statements)

References 108 publications

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“…Iontophoresis is a non-invasive drug delivery technology enhancing the permeation of ionized molecules across biological barriers using a continuous low-level electrical field [ 58 ]. This strategy has been proven useful for transdermal drug delivery to facilitate percutaneous penetration [ 59 , 60 ] and became an attractive option for drug and gene delivery in the eye [ 61 ]. Several studies have assessed transcorneal and transscleral iontophoresis-assisted plasmid DNA transfer for non-viral ocular gene therapy; however, limited expression was produced, especially in the photoreceptor cells [ 58 , 62 ].…”

Section: Physical Methods Of Transfectionmentioning

confidence: 99%

The Landscape of Non-Viral Gene Augmentation Strategies for Inherited Retinal Diseases

Toualbi

Toms

Moosajee

2021

IJMS

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Inherited retinal diseases (IRDs) are a heterogeneous group of disorders causing progressive loss of vision, affecting approximately one in 1000 people worldwide. Gene augmentation therapy, which typically involves using adeno-associated viral vectors for delivery of healthy gene copies to affected tissues, has shown great promise as a strategy for the treatment of IRDs. However, the use of viruses is associated with several limitations, including harmful immune responses, genome integration, and limited gene carrying capacity. Here, we review the advances in non-viral gene augmentation strategies, such as the use of plasmids with minimal bacterial backbones and scaffold/matrix attachment region (S/MAR) sequences, that have the capability to overcome these weaknesses by accommodating genes of any size and maintaining episomal transgene expression with a lower risk of eliciting an immune response. Low retinal transfection rates remain a limitation, but various strategies, including coupling the DNA with different types of chemical vehicles (nanoparticles) and the use of electrical methods such as iontophoresis and electrotransfection to aid cell entry, have shown promise in preclinical studies. Non-viral gene therapy may offer a safer and effective option for future treatment of IRDs.

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Section: Physical Methods Of Transfectionmentioning

confidence: 99%

The Landscape of Non-Viral Gene Augmentation Strategies for Inherited Retinal Diseases

Toualbi

Toms

Moosajee

2021

IJMS

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“…The pHs of acute wounds and the microenvironment of skin cancer are known to be acidic, while chronic wounds and infected wounds with a high bacterial burden usually exhibit pH values above 7.3 252 . Currently, in order to improve skin permeation and stability of drugs, many transdermal drug delivery systems are currently under investigation, including nano- or micro-particles 253 , 254 , 255 , hydrogels 254 , 256 , 257 , and microneedles 258 , 259 , 260 , 261 .…”

Section: Mdps-based Formulations and Their Applicationsmentioning

confidence: 99%

Membrane-disruptive peptides/peptidomimetics-based therapeutics: Promising systems to combat bacteria and cancer in the drug-resistant era

Lin

Chi

Yan

et al. 2021

Acta Pharmaceutica Sinica B

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Membrane-disruptive peptides/peptidomimetics (MDPs) are antimicrobials or anticarcinogens that present a general killing mechanism through the physical disruption of cell membranes, in contrast to conventional chemotherapeutic drugs, which act on precise targets such as DNA or specific enzymes. Owing to their rapid action, broad-spectrum activity, and mechanisms of action that potentially hinder the development of resistance, MDPs have been increasingly considered as future therapeutics in the drug-resistant era. Recently, growing experimental evidence has demonstrated that MDPs can also be utilized as adjuvants to enhance the therapeutic effects of other agents. In this review, we evaluate the literature around the broad-spectrum antimicrobial properties and anticancer activity of MDPs, and summarize the current development and mechanisms of MDPs alone or in combination with other agents. Notably, this review highlights recent advances in the design of various MDP-based drug delivery systems that can improve the therapeutic effect of MDPs, minimize side effects, and promote the co-delivery of multiple chemotherapeutics, for more efficient antimicrobial and anticancer therapy.

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“…Physical enhancers: Iontophoresis is a non-invasive permeation technology that physically facilitates transdermal delivery of hydrophilic and ionic compounds across intact skin [7]. The ultrasound method interacts with the structural lipids present in the intercellular channels of the stratum corneum, which is similar to the postulated impacts of some chemical transdermal enhancers, which act through disrupting lipids.…”

Section: Enhancer Permeationmentioning

confidence: 99%

“…Increases penetration through the skin; gives systemic and local effects [8] Only for drugs with low molecular weight; skin irritation Immunogenicity [8] Iontophoresis Increasing the penetration of drug of intermediate size [7] Low transfer efficiency; only for charged drugs [8] Ultrasound At low frequencies, increase skin permeability does not induce the transport of high molecular weight proteins [9].…”

Section: Chemical Enhancermentioning

confidence: 99%