Noninvasive testing of preimplantation embryos in assisted reproductive technology

Abstract

One approach to improving the success of assisted reproductive technology (ART) is the careful selection of embryos prior to implantation. Although preimplantation genetic testing (PGT) is widely employed for embryo selection, it needs embryo biopsy and is detrimental to embryos. Thus, noninvasive testing of preimplantation embryos offers new possibilities for evaluating embryo quality. Here, we reviewed current progression of noninvasive embryo testing technologies, including the use of microscopy images combined with artificial intelligence (AI) to select embryos based on morphology, minimally invasive and noninvasive PGT of blastocoel fluid and spent embryo culture medium, and omics analysis of molecules in the culture medium to assess the developmental potential of embryos. More importantly, using the AI technology based on various type of data of each embryo will greatly improve the noninvasive embryo assessments. Thus, these cutting-edge technologies offer fresh insights into noninvasive testing of preimplantation embryos and have the potential to enhance the quality and efficiency of ART procedures.