Noninvasive real-time imaging of intima thickness after rat carotid artery balloon injury using ultrasound biomicroscopy

Razuvaev, Anton; Lund, Kent; Roy, Joy; Hedin, Ulf; Caidahl, Kenneth

doi:10.1016/j.atherosclerosis.2007.11.035

Cited by 34 publications

(27 citation statements)

References 18 publications

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“…Ultrasound biomicroscopy has been shown to be a useful tool for the assessment of intimal hyperplasia and lumen diameter in the rat carotid injury model [19]. In this study, a high-resolution ultrasound system (Vevo 2100; Visualsonics Inc., Toronto, Ont., Canada), equipped with a MS700 transducer (up to 70 MHz), was used for in vivo evaluation of intimal hyperplasia and lumen diameter of the carotid arteries 2 and 4 weeks after the injury.…”

Section: Methodsmentioning

confidence: 99%

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Glucagon-Like Peptide-1 Receptor Activation Does Not Affect Re-Endothelialization but Reduces Intimal Hyperplasia via Direct Effects on Smooth Muscle Cells in a Nondiabetic Model of Arterial Injury

Eriksson

Saxelin²,

Röhl³

et al. 2015

J Vasc Res

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Diabetic patients have an increased risk of restenosis and late stent thrombosis after angioplasty, i.e. complications that are related to a defective re-endothelialization. Exendin-4, a stable glucagon-like peptide (GLP)-1 receptor agonist, has been suggested to influence the formation of intimal hyperplasia and to increase endothelial cell proliferation in vitro. Thus, the aim of this study was to investigate the mechanisms by which treatment with exendin-4 could influence re-endothelialization and intimal hyperplasia after vascular injury. Methods: Sprague-Dawley rats were subjected to balloon injury of the left common carotid artery and treated for 4 weeks with exendin-4 or vehicle. Intimal hyperplasia and vessel wall elasticity were monitored noninvasively by high-frequency ultrasound, and re-endothelialization was evaluated upon sacrifice using Evans blue dye. Results and Conclusion: Exendin-4 selectively reduced the proliferation of smooth muscle cells (SMCs) and intimal hyperplasia in vivo without affecting the re-endothelialization process, but treatment with exendin-4 improved arterial wall elasticity. Our data also show that exendin-4 significantly decreased the proliferation and increased the apoptosis of SMCs in vitro, effects that appear to be mediated through cAMP signaling and endothelial nitric oxide synthase following GLP-1 receptor activation. Together, these effects of exendin-4 are highly desirable and may lead to an improved outcome for patients undergoing vascular interventions.

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Section: Methodsmentioning

confidence: 99%

“…The passage was repeated three times in order to remove the endothelial layer. The external carotid artery was then ligated and the skin wound closed [19]. The animal was then turned onto its stomach, the osmotic ALZET® pump was placed subcutaneously on the back and the skin wound was closed.…”

Section: Methodsmentioning

confidence: 99%

Glucagon-Like Peptide-1 Receptor Activation Does Not Affect Re-Endothelialization but Reduces Intimal Hyperplasia via Direct Effects on Smooth Muscle Cells in a Nondiabetic Model of Arterial Injury

Eriksson

Saxelin²,

Röhl³

et al. 2015

J Vasc Res

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“…6 UBM measurements of IMT and plaque thickness in various animal studies have been compared with histological data showing close correlations between the methods. 7,8 Moreover, we have published the results of in vitro measurements of IT and MT of human mesenteric arteries by UBM, demonstrating close correlations to histological measurements. 9 In that study the discrimination capacity of UBM was tested with silicon layer phantoms (measuring 1-200 μm) showing high accuracy.…”

Section: Introductionmentioning

confidence: 99%

Increased intima thickness of the radial artery in patients with coronary heart disease

et al. 2009

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Ultrabiomicroscopy is a novel high-frequency (55 MHz) ultrasound technique that could be used to non-invasively measure the vessel wall and separate the intima-media complex into measurements of intima and media thickness. Since no previous study has measured intima and media thickness separately in vivo in patients with coronary heart disease (CHD), the aim of the current study was to measure intima and intima-media thickness of the radial and the anterior tibial arteries among patients with CHD and healthy subjects (HS). Thirty-two patients with CHD and 46 HS underwent investigations with ultrabiomicroscopy measurements of the radial and anterior tibial arteries. Patients with CHD showed a 19% increase in intima thickness of the radial artery compared with HS (0.088  0.024 mm versus 0.074  0.015 mm; p < 0.015), whereas no difference was seen in media thickness. There were no differences in intima or media thickness within the anterior tibial arteries. In conclusion, CHD is associated with thickening of the intima of the radial artery whereas media thickness was unchanged compared with HS. Assessment of intima thickness by highfrequency ultrasound may provide a tool for non-invasive early detection of atherosclerosis.

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“…Carotid artery balloon injury in rats is a well-established model to study the initial response to vascular injury 20 and triggers remodeling and neointima formation via SMC proliferation and survival as depicted in Figure 2A.…”

Section: Mir-210 Is Repressed In Experimental Carotid Artery Remodelingmentioning

confidence: 99%

“…Injury of the common carotid artery in rats was performed as previously described 20 in accordance with the Guide for the Care and Use of Laboratory Animals published by the US National Institutes of Health (NIH Publication No. 85-23, revised 1996).…”

Section: Rat Carotid Artery Balloon Injury Modelmentioning

confidence: 99%

MicroRNA-210 Enhances Fibrous Cap Stability in Advanced Atherosclerotic Lesions

Eken

Jin

Chernogubova

et al. 2017

Circulation Research

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108

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Stroke is the second most common cause of death 2 and major cause of disability worldwide, 2 with survivors often depending on lifelong care. Carotid stenosis is the second most common predecessor of ischemic stroke, 3 and carotid endarterectomy (CEA; and to a lesser extent carotid artery stenting) are accepted as secondary, but also primary preventive therapy. 2,4,5 With new imaging techniques, vulnerable plaques at risk of rupture can be identified with increasing accuracy, 6 but in the majority of cases, the perioperative surgical risk of ≈3% still outweighs the risk of plaque rupture in asymptomatic carotid stenosis carriers.7 As a result, CEA and carotid artery stenting are currently unfavorable for most of these individuals.8 Detection and stabilization of a vulnerable plaque by influencing the local disease process biologically would provide a sophisticated solution for asymptomatic plaque carriers at risk of stroke. Molecular Medicine© 2016 American Heart Association, Inc. Conclusions: An unstable carotid plaque at risk of stroke is characterized by low expression of miR-210. miR-210 contributes to stabilizing carotid plaques through inhibition of APC, ensuring smooth muscle cell survival. We present local delivery of miR-210 as a therapeutic approach for prevention of atherothrombotic vascular events.

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Noninvasive real-time imaging of intima thickness after rat carotid artery balloon injury using ultrasound biomicroscopy

Cited by 34 publications

References 18 publications

Glucagon-Like Peptide-1 Receptor Activation Does Not Affect Re-Endothelialization but Reduces Intimal Hyperplasia via Direct Effects on Smooth Muscle Cells in a Nondiabetic Model of Arterial Injury

Glucagon-Like Peptide-1 Receptor Activation Does Not Affect Re-Endothelialization but Reduces Intimal Hyperplasia via Direct Effects on Smooth Muscle Cells in a Nondiabetic Model of Arterial Injury

Increased intima thickness of the radial artery in patients with coronary heart disease

MicroRNA-210 Enhances Fibrous Cap Stability in Advanced Atherosclerotic Lesions

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