Noninvasive, quantitative fluorescence sensing in 3D tissues: an approach to in vivo molecular characterization of engineered tissue constructs

Chandra, Mudit; Vishwanath, Karthik; Fichter, G.; Liao, Elly E.; Francis, Michael R.; Hollister, Scott J.; Mycek, Mary Ann

doi:10.1364/bio.2006.me59

Cited by 1 publication

(1 citation statement)

References 6 publications

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“…The system has several features that are essential for eventual clinical translation [5][6][7][8][9][10][11] and pre-clinical tissue engineering applications [12][13][14][15][16]: the system is compact, easy to use, low cost, and measures high-quality, information-rich fluorescence WTMs. The WTMs measured from a tissue-simulating phantom demonstrates the ability of the instrument to resolve exogenous or endogenous fluorescence from multiple fluorophores and detect distinct differences from timeresolved decays at several distinct wavelengths.…”

Section: Discussionmentioning

confidence: 99%

Fluorescence wavelength-time matrix acquisition for biomedical tissue diagnostics

et al. 2011

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A specialized transient digitizer system was developed for spectroscopic collection of fluorescence wavelength-time matrices (WTMs) from biological tissues. The system is compact, utilizes fiber optic probes for clinical compatibility, and offers rapid collection of high signal-to-noise ratio (>100) time-and wavelength-resolved fluorescence. The system is compatible with excitation sources operating in excess of 25 kHz. Wavelength-resolved measurement range is 300-800 nm with ≥ 0.01 nm steps. Time-resolved measurement depth is 128 ns with fixed 0.2 ns steps. The information-rich WTM data provides comprehensive fluorescence sensing capabilities, as demonstrated on tissue simulating phantoms. Extracting wavelength-resolved fluorophore lifetimes illustrates the potential of using the technology to resolve exogenous or endogenous fluorophore contributions in tissue samples in a clinical setting for tissue diagnostics and monitoring.

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