Noninvasive Optical Imaging of <i>Staphylococcus aureus</i> Bacterial Infection in Living Mice Using a Bis-Dipicolylamine-Zinc(II) Affinity Group Conjugated to a Near-Infrared Fluorophore

Leevy, W. Matthew; Gammon, Seth T.; Johnson, James R.; Lampkins, Andrew J.; Jiang, Hua; Márquez, Manuel; Piwnica‐Worms, David; Suckow, Mark A.; Smith, Bradley D.

doi:10.1021/bc700376v

Cited by 97 publications

(90 citation statements)

References 31 publications

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“…The use of an injectable NIR probe consisting of a bacterial affinity group conjugated to an NIR dye is recently demonstrating success. 85,86 Maltodextrin-based optical imaging probes have been shown to successfully detect small numbers of bacteria in vivo with very high specificity and selectivity due to the fact that maltodextrin transporter is unique to bacteria. 87 A further advantage to this strategy is that the lumen of intestinal tissues and the skin are not permeable to glucose oligomers, and therefore MDPs delivered systemically should not be internalized by the resident bacterial microflora.…”

Section: Instrumentation For Oimentioning

confidence: 99%

“…Similarly, bis-zinc(II)-dicolylamide ligands have high affinity for the anionic phospholipids and other molecules residing in bacteria. 86 Xenolight RediJect probe sold by Caliper is a NIR fluorescent agent for in vivo detection of bacteria targeting the anionic phospholipids of bacterial cell membranes. The potential to adapt these technologies to image bacteria colonising tumors is exciting; however this approach may only be applicable to subcutaneous surface tumors.…”

Section: Instrumentation For Oimentioning

confidence: 99%

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Bacterial vectors for imaging and cancer gene therapy: a review

et al. 2012

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The significant burden of resistance to conventional anticancer treatments in patients with advanced disease has prompted the need to explore alternative therapeutic strategies. The challenge for oncology researchers is to identify a therapy which is selective for tumors with limited toxicity to normal tissue. Engineered bacteria have the unique potential to overcome traditional therapies' limitations by specifically targeting tumors. It has been shown that bacteria are naturally capable of homing to tumors when systemically administered resulting in high levels of replication locally, either external to (non-invasive species) or within tumor cells (pathogens). Pre-clinical and clinical investigations involving bacterial vectors require relevant means of monitoring vector trafficking and levels over time, and development of bacterial-specific real-time imaging modalities are key for successful development of clinical bacterial gene delivery. This review discusses the currently available imaging technologies and the progress to date exploiting these for monitoring of bacterial gene delivery in vivo.Cancer Gene Therapy ( INTRODUCTIONAlthough the potential anti-cancer effects of acquired bacterial infection have been evident for over 120 years and the presence of bacteria in excised tumors has been noted for over 60 years, 1 it is only in more recent times that the tumor-specific growth of bacteria has been considered for therapeutic purposes. While the precise mechanism(s) behind preferential bacterial tumor colonization remain poorly understood, this phenomenon must relate to unique tumor attributes that separate them from healthy tissues. Factors such as the irregular blood supply; local immune suppression; inflammation; and unique nutrient supply (e.g., purines) in tumors have been proposed to play a role. 2 Bacterial entry into the tumor environment is proposed to be facilitated by the leaky vasculature. Cancer cells are characterized by an aberrantly accelerated metabolism and proliferation leading to an imbalance of oxygen supply and consumption which is a major causative factor of tumor hypoxia. 3 The hypoxic nature of solid tumors enhances the growth of anaerobic and facultatively anaerobic bacteria. In addition, these areas with low oxygen and high interstitial pressure are considered to be an immunological sanctuary, where bacterial clearance mechanisms are greatly inhibited. 4 Necrotic regions are also rich in nutrients favoured by bacteria due to tumor cell turnover. However, tumor selective bacterial colonization now appears to be both bacterial species and tumor origin independent, since aerobic bacteria are also capable of colonising tumors, and even small tumors lacking an anaerobic center are colonised. See Figure 1 for proposed mechanisms.Invasive bacteria can internalize and replicate within tumor cells, while non-invasive bacteria grow externally to tumor cells, within the tumor micro-environment. 5 The tumor selective growth of bacteria has made them an attractive vehicle for the delivery of ...

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Section: Instrumentation For Oimentioning

confidence: 99%

Section: Instrumentation For Oimentioning

confidence: 99%

Bacterial vectors for imaging and cancer gene therapy: a review

et al. 2012

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“…18 One approach is to use bacteria that are genetically encoded to produce luciferase or green°u orescent protein. A second strategy, which is the focus of this study, employs a molecular°uorescent probe.…”

Section: à10mentioning

confidence: 99%

NONINVASIVE OPTICAL IMAGING OF STAPHYLOCOCCUS AUREUS INFECTION IN VIVO USING AN ANTIMICROBIAL PEPTIDE FRAGMENT BASED NEAR-INFRARED FLUORESCENT PROBES

Liu

2013

J. Innov. Opt. Health Sci.

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The diagnosis of bacterial infections remains a major challenge in medicine. Optical imaging of bacterial infection in living animals is usually conducted with genetic reporters such as lightemitting enzymes or°uorescent proteins. However, there are many circumstances where genetic reporters are not applicable, and there is an urgent need for exogenous synthetic probes that can selectively target bacteria. Optical imaging of bacteria in vivo is much less developed than methods such as radioimaging and MRI. Furthermore near-infrared (NIR) dyes with emission wavelengths in the region of 650À900 nm can propagate through two or more centimeters of tissue and may enable deeper tissue imaging if sensitive detection techniques are employed. Here we constructed an antimicrobial peptide fragment UBI29-41-based near-infrared°uorescent imaging probe. The probe is composed of UBI29-41 conjugated to a near infrared dye ICG-Der-02. UBI29-41 is a cationic antimicrobial peptide that targets the anionic surfaces of bacterial cells. The probe allows detection of Staphylococcus aureus infection (5Â10 7 cells) in a mouse local infection model using whole animal near-infrared°uorescence imaging. Furthermore, we demonstrate that the UBI29-41-based imaging probe can selectively accumulate within bacteria. The signi¯cantly higher accumulation in bacterial infection suggests that UBI29-41-based imaging probe may be a promising imaging agent to detect bacterial infections.

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“…Increased research interest in cyanine dyes chemistry , Shindy et al, 2016, Shindy et al, 2015a, Shindy et al, 2014 essentially arises from their applications and uses in various research field (Shershof, et al, 2013, Leevy, et al, 2008, Zhang, et al, 2004and Savitsky, et al 2004, Li et al, 2006, Wang, et al, 2007, Licha, et al, 2000. Such as biochemistry, molecular biology, bio-medicine and structural biology.…”

Section: Introductionmentioning

confidence: 99%

Synthesis, Spectral Sensitization, Solvatochromic and Halochromic Evaluation of New Monomethine and Trimethine Cyanine Dyes

2017

European Journal of Molecular Biotechnology

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New heterocyclic starting material namely 4,5-dimethyl-2,7-diphenyl-furo [(3,2-bis pyrazole were prepared and employed to synthesis of some novel monomethine cyanine dyes (simple cyanine dyes) and trimethine cyanine dyes (carbocyanine dyes). Spectral sensitization evaluation for all the synthesized cyanine dyes was carried out through investigating their electronic visible absorption spectra in 95% ethanol solution. Solvatochromic and/or hachromic evaluation for some selected dyes were carried out through examining their electronic visible absorption spectra in pure solvents having different polarities [Water (78.54), Dimethylformamide (36.70), Ethanol (24.3), Chloroform (4.806), Carbontetrachloride (2.238) and Dioxane (2.209)] and/or in aqueous universal buffer solutions having varied pH values (1.75, 2.45, 4.65, 5.80, 7.88, 8.75, 10.85 and 12.60 units), respectively. Structure determination were carried out via elemental analysis, visible spectra. Mass spectrometer, IR and 1 H NMR spectral data.

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Noninvasive Optical Imaging of Staphylococcus aureus Bacterial Infection in Living Mice Using a Bis-Dipicolylamine-Zinc(II) Affinity Group Conjugated to a Near-Infrared Fluorophore

Cited by 97 publications

References 31 publications

Bacterial vectors for imaging and cancer gene therapy: a review

Bacterial vectors for imaging and cancer gene therapy: a review

NONINVASIVE OPTICAL IMAGING OF STAPHYLOCOCCUS AUREUS INFECTION IN VIVO USING AN ANTIMICROBIAL PEPTIDE FRAGMENT BASED NEAR-INFRARED FLUORESCENT PROBES

Synthesis, Spectral Sensitization, Solvatochromic and Halochromic Evaluation of New Monomethine and Trimethine Cyanine Dyes

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