Noninvasive Observations of Fluorinated Anesthetics in Rabbit Brain by Fluorine-19 Nuclear Magnetic Resonance

Wyrwicz, Alice M.; Pszenny, M. H.; Schofield, J.; Tillman, Philip; Gordon, R. E.; Martin, Peter A.; Munson, Edwin S.

doi:10.1097/00132582-198400410-00024

Cited by 7 publications

(12 citation statements)

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“…19F spectra of anaesthetic agents have been reported in model systems (see, for example, Koehler, Jain, Stone, Fossel & Koehler, 1978), in blood (Wyrwicz, Schofield & Burt, 1982), and in biological tissues (Wyrwicz, Pszenny, Schofield, Tillman, Gordon & Martin, 1983;Burt, Moore, Roberts & Brady, 1984;Moore & Roberts, 1985). The 19F chemical shift of halothane shows a pronounced dependence on tissue type, and at least in excised tissues there are demonstrative differences between tumour and non-tumour tissue.…”

Section: F Studiesmentioning

confidence: 99%

TISSUE METABOLISM STUDIED IN VIVO BY NUCLEAR MAGNETIC RESONANCE

Williams

Gadian

1986

Exp Physiol

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N.m.r. is finding increasing application as a non‐invasive method of studying metabolism in vivo. 31P n.m.r. is particularly useful for studies of energy metabolism, while other nuclei, in particular 1H, 13C and 19F, can provide additional information about certain aspects of cellular metabolism that are not amenable to investigation by 31P n.m.r. The major problem with n.m.r. is lack of sensitivity. The main implications of this are first that signals can only be detected from metabolites that are present at fairly high concentrations, and secondly that the spatial resolution for localized studies, particularly of internal regions of the body, is limited. N.m.r. could considerably enhance our understanding of metabolic abnormalities in disease, but the precise role of such metabolic studies in a clinical setting remains to be evaluated.

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Section: F Studiesmentioning

confidence: 99%

TISSUE METABOLISM STUDIED IN VIVO BY NUCLEAR MAGNETIC RESONANCE

Williams

Gadian

1986

Exp Physiol

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“…Moreover, among the volatile halogenated anesthetics, isoflurane seems to undergo the least amount of defluorination (Kharasch and Thummel, 1993). Almost 30 years ago, it was shown that high-resolution 19 F magnetic resonance spectra from isoflurane could be obtained from a surface coil centered over the brain of a living rabbit (Wyrwicz et al, 1983).…”

Section: Introductionmentioning

confidence: 99%

In Vivo Magnetic Resonance Imaging to Detect Biliary Excretion of ¹⁹F-Labeled Drug in Mice

Raufman¹,

Xu²,

Cheng³

et al. 2011

Drug Metab Dispos

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“…These peaks may interfere with the detection of fluorinated agents in vivo (chemical shift range of -126.6-9.1 ppm with respect to CFCl 3 and -50-86 ppm with respect to trifluoroacetic acid [TFA]) due to potential spectral overlaps. Furthermore, despite the existence of numerous prior 19 F MRI studies on the uptake and clearance characteristics of ISO in the brain in vivo, [14][15][16][17] the localization of ISO in cardiac thoracic and peripheral skeletal tissues, its bio-distribution, accumulation, kinetics, and possible interference with 19 F labels has not been investigated in detail. Additionally, only a few reported cardiovascular applications of fluorinated agents exist, 18,19 and investigations into the possible implications of the use of ISO have been speculative.…”

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confidence: 99%

Temporal accumulation and localization of isoflurane in the C57BL/6 mouse and assessment of its potential contamination in ¹⁹F MRI with perfluoro‐crown‐ether‐labeled cardiac progenitor cells at 9.4 Tesla

Constantinides

Maguire

Stork

et al. 2016

Magnetic Resonance Imaging

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PurposeTo assess the uptake, accumulation, temporal stability, and spatial localization of isoflurane (ISO) in the C57BL/6 mouse, and to identify its potential interference with the detection of labeled cardiac progenitor cells using 19F MRI/MR spectroscopy (MRS).Materials and MethodsObjectives are demonstrated using (a) in vitro ISO tests, (b) in vivo temporal accumulation/spatial localization C57BL/6 studies (n = 3), and (c) through injections of perfluoro‐crown‐ether (PFCE) labeled cardiac progenitor cells into femoral muscle areas of the murine hindlimb post‐mortem (n = 1) using 1H/19F MRI/MRS at 9.4 Tesla. Data were acquired using double‐gated spoiled gradient echo images and pulse‐acquire spectra. For the in vivo study, the temporal stability of ISO resonances was quantified using coefficient of variability (CV) (5 min) estimates.ResultsTwo ISO resonances were observed in vivo that correspond to the ‐CF3 and ‐OCHF2 moieties. CV values ranged between 3.2 and 6.4% (‐CF3) and 6.4 and 11.2% (‐OCHF2). Reductions of the ISO dose (2.0 to 1.7%) at 80 min postinduction had insignificant effects on ISO signals (P = 0.23; P = 0.71). PFCE‐labeled cells exhibited a resonance at −16.25 ppm in vitro that did not overlap with the ISO resonances, a finding that is confirmed with MRS post‐mortem using injected, labeled cells. Based on 19F MRI, similar in vivo/post‐mortem ISO compartmentalization was also confirmed in peripheral and thoracic skeletal muscles.ConclusionSignificant ISO accumulation was observed by 19F MRS in vivo with temporally stable signals over 90 min postinduction. ISO effects on PFCE labels are anticipated to be minimal but may be more prominent for perfluoropolyether or perfluorooctyl bromide labels. Level of Evidence: 1 Technical Efficacy: Stage 1J. MAGN. RESON. IMAGING 2017;45:1659–1667

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Noninvasive Observations of Fluorinated Anesthetics in Rabbit Brain by Fluorine-19 Nuclear Magnetic Resonance

Cited by 7 publications

References 0 publications

TISSUE METABOLISM STUDIED IN VIVO BY NUCLEAR MAGNETIC RESONANCE

TISSUE METABOLISM STUDIED IN VIVO BY NUCLEAR MAGNETIC RESONANCE

In Vivo Magnetic Resonance Imaging to Detect Biliary Excretion of ¹⁹F-Labeled Drug in Mice

Temporal accumulation and localization of isoflurane in the C57BL/6 mouse and assessment of its potential contamination in ¹⁹F MRI with perfluoro‐crown‐ether‐labeled cardiac progenitor cells at 9.4 Tesla

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Noninvasive Observations of Fluorinated Anesthetics in Rabbit Brain by Fluorine-19 Nuclear Magnetic Resonance

Cited by 7 publications

References 0 publications

TISSUE METABOLISM STUDIED IN VIVO BY NUCLEAR MAGNETIC RESONANCE

TISSUE METABOLISM STUDIED IN VIVO BY NUCLEAR MAGNETIC RESONANCE

In Vivo Magnetic Resonance Imaging to Detect Biliary Excretion of 19F-Labeled Drug in Mice

Temporal accumulation and localization of isoflurane in the C57BL/6 mouse and assessment of its potential contamination in 19F MRI with perfluoro‐crown‐ether‐labeled cardiac progenitor cells at 9.4 Tesla

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In Vivo Magnetic Resonance Imaging to Detect Biliary Excretion of ¹⁹F-Labeled Drug in Mice

Temporal accumulation and localization of isoflurane in the C57BL/6 mouse and assessment of its potential contamination in ¹⁹F MRI with perfluoro‐crown‐ether‐labeled cardiac progenitor cells at 9.4 Tesla