Noninvasive monitoring of glucose concentration with optical coherence tomography

Esenaliev, Rinat O.; Larin, Krill V.; Larina, Irina V.; Motamedi, Massoud

doi:10.1364/ol.26.000992

Cited by 194 publications

(170 citation statements)

References 6 publications

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“…On average, the slope changed 1.9% per 10 mg/dl in these studies. The results of these tests are in good agreement with those obtained in our animal studies (17,18).…”

Section: Analytical Proceduressupporting

confidence: 90%

“…Our previous studies performed in tissue phantoms and animals in vivo with an OCT system showed high accuracy of measurements of changes in the scattering coefficient in tissue phantoms and a good correlation between changes in glucose concentration and the OCT signal slope in animals (17,18). In this pilot study, we tested the feasibility of noninvasive glucose monitoring with this OCT system in healthy human subjects.…”

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confidence: 94%

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Noninvasive Blood Glucose Monitoring With Optical Coherence Tomography

et al. 2002

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OBJECTIVE—To study the feasibility of noninvasive blood glucose monitoring using optical coherence tomography (OCT) technique in healthy volunteers. RESEARCH DESIGN AND METHODS—An OCT system with the wavelength of 1,300 nm was used in 15 healthy subjects in 18 clinical experiments. Standard oral glucose tolerance tests were performed to induce changes in blood glucose concentration. Blood samples were taken from the right arm vein every 5 or 15 min. OCT images were taken every 10–20 s from the left forearm over a total period of 3 h. The slope of the signals was calculated at the depth of 200–600 μm from the skin surface. RESULTS—A total of 426 blood samples and 8,437 OCT images and signals were collected and analyzed in these experiments. There was a good correlation between changes in the slope of noninvasively measured OCT signals and blood glucose concentrations throughout the duration of the experiments. The slope of OCT signals changed significantly (up to 2.8% per 10 mg/dl) with variation of plasma glucose values. The good correlation obtained between the OCT signal slope and blood glucose concentration is due to the coherent detection of backscattered photons, which allows measurements of OCT signal from a specific tissue layer without unwanted signal from other tissue layers. CONCLUSIONS—This pilot study demonstrated the capability of the OCT technique to monitor blood glucose concentration noninvasively in human subjects. Further studies with a larger number of subjects including diabetic subjects are planned to validate these preliminary results.

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“…On average, the slope changed 1.9% per 10 mg/dl in these studies. The results of these tests are in good agreement with those obtained in our animal studies (17,18).…”

Section: Analytical Proceduressupporting

confidence: 90%

mentioning

confidence: 94%

Noninvasive Blood Glucose Monitoring With Optical Coherence Tomography

et al. 2002

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“…This is consistent with our study in which collagen solubility predicted glucose to have twice the OCP of glycerol. In addition, it is interesting to note that in efforts to develop non-invasive, optical monitoring of blood glucose levels, a correlation has been reported between a reduction in tissue scattering and glucose concentration [19][20][21]. The reduction in tissue scattering was hypothesized to arise from an increase in glucose concentration in local extracellular space, increasing its refractive index to better match that of tissue scatterers.…”

Section: Discussionmentioning

confidence: 99%

Correlation between collagen solubility and skin optical clearing using sugars

et al. 2007

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Background and Objective: Light scattering from collagen within skin limits light-based therapeutics while increasing the risk of epidermal thermal injury. Specific chemicals show the ability to reduce light scattering by reversibly altering the optical properties of skin. This study examines the correlation between collagen solubility and the optical clearing potential (OCP) of sugars and sugar-alcohols using in vitro rodent skin. Materials and Methods: Collagen solubility in dextrose, fructose, sucrose, and sorbitol was measured using near-UV spectroscopy. Light transmittance, reflectance, and rodent skin thickness were measured (giving skin reduced scattering coefficient) before and after exposure of the dermal surface to sugars and sugar-alcohols. OCP was calculated as the ratio of reduced scattering coefficients before and after exposures. Results: Dextrose, fructose, sucrose, and sorbitol had at least twice the collagen solubility and twice the OCP as compared to glycerol. In general, collagen solubility correlated with each agent's ability to optically clear rodent skin.Conclusion: These results demonstrate that sugar and sugar-alcohol interaction with collagen are a primary event in tissue optical clearing.

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“…The sensitivity of xfOCM should prove useful to monitor changes in beta cell number-for example, during islet regeneration [30] or upon islet transplantation to visualise the islets and their vascularisation in the recipient. Analysing dispersion and spectral absorption characteristics in the sample could add further functional contrast such as variations in metabolite concentrations [31,32]. Eventually, xfOCM could help design new PET and MRI probes that selectively label the pancreatic beta cells and that are necessary for clinical imaging of pancreatic islets by these techniques.…”

Section: Discussionmentioning

confidence: 99%

In vivo imaging of murine endocrine islets of Langerhans with extended-focus optical coherence microscopy

et al. 2009

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Aims/hypothesis Structural and functional imaging of the islets of Langerhans and the insulin-secreting beta cells represents a significant challenge and a long-lasting objective in diabetes research. In vivo microscopy offers a valuable insight into beta cell function but has severe limitations regarding sample labelling, imaging speed and depth, and was primarily performed on isolated islets lacking native innervations and vascularisation. This article introduces extended-focus optical coherence microscopy (xfOCM) to image murine pancreatic islets in their natural environment in situ, i.e. in vivo and in a label-free condition.Methods Ex vivo measurements on excised pancreases were performed and validated by standard immunohistochemistry to investigate the structures that can be observed with xfOCM. The influence of streptozotocin on the signature of the islets was investigated in a second step. Finally, xfOCM was applied to make measurements of the murine pancreas in situ and in vivo. Results xfOCM circumvents the fundamental physical limit that trades lateral resolution for depth of field, and achieves fast volumetric imaging with high resolution in all three dimensions. It allows label-free visualisation of pancreatic lobules, ducts, blood vessels and individual islets of Langerhans ex vivo and in vivo, and detects streptozotocin-induced islet destruction. Conclusions/interpretation Our results demonstrate the potential value of xfOCM in high-resolution in vivo studies to assess islet structure and function in animal models of diabetes, aiming towards its use in longitudinal studies of diabetes progression and islet transplants.

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Noninvasive monitoring of glucose concentration with optical coherence tomography

Cited by 194 publications

References 6 publications

Noninvasive Blood Glucose Monitoring With Optical Coherence Tomography

Noninvasive Blood Glucose Monitoring With Optical Coherence Tomography

Correlation between collagen solubility and skin optical clearing using sugars

In vivo imaging of murine endocrine islets of Langerhans with extended-focus optical coherence microscopy

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