Noninvasive molecular fingerprinting of host–microbiome interactions in neonates

Donovan, Sharon M.; Wang, Mei; Monaco, Marcia H.; Martin, Camilia R.; Davidson, Laurie A.; Ivanov, Ivan; Chapkin, Robert S.

doi:10.1016/j.febslet.2014.07.008

Cited by 34 publications

(28 citation statements)

References 84 publications

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“…The development of the intestinal immune system starts in early natal life (Steege et al 1997), and the postnatal period is a critical window for maturation, since the microbiota composition diverges from the maternal one (Donovan et al 2014). Hornef and Fulde (2014), showed the differential expression of immunity genes in the murine neonate and adult intestine, and age-related transformations have also been reported for adaptive immunity (Howie et al 1998;He et al 2013).…”

Section: Discussionmentioning

confidence: 99%

miRNA-regulated gene expression differs in celiac disease patients according to the age of presentation

et al. 2015

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Celiac disease is an intestinal disease which shows different symptoms and clinical manifestations among pediatric and adult patients. These variations could be imputable to age-related changes in gut architecture and intestinal immune system, which could be characterized by gene expression differences possibly regulated by miRNAs. We analyzed a panel of miRNAs and their target genes in duodenal biopsies of Marsh 3AB and 3C pediatric celiac patients, compared to controls. Moreover, to assess variation of expression in plasma samples, we evaluated circulating miRNA levels in controls and patients at diagnosis or on gluten-free diet. We detected a decreased miR-192-5p expression in celiac patients, but no variations in NOD2 and CXCL2, targets previously identified in adults. Conversely, we detected a significant increase in mRNA and protein levels of another target, MAD2L1, protein related to cell cycle control. miR-31-5p and miR-338-3p were down-regulated and their respective targets, FOXP3 and RUNX1, involved in Treg function, resulted up-regulated in celiac patients. Finally, we detected, in celiac patients, an increased expression of miR-21-5p, possibly caused by a regulatory loop with its putative target STAT3, which showed an increased activation in Marsh 3C patients. The analysis of plasma revealed a trend similar to that observed in biopsies, but in presence of gluten-free diet we could not detect circulating miRNAs values comparable to controls. miRNAs and their gene targets showed an altered expression in duodenal mucosa and plasma of celiac disease pediatric patients, and these alterations could be different from adult ones.

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Section: Discussionmentioning

confidence: 99%

miRNA-regulated gene expression differs in celiac disease patients according to the age of presentation

et al. 2015

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“…Metagenomic analyses have also contributed to remarkable progress in uncovering the impact of maternal and newborn diets on changes in GI microbiota [65,66], including differential gene expression and functional outcomes as seen between in formula-fed and the breast-fed infants [65,66,67]. These include findings on differential expression of immune-related genes in formula-fed and the breast-fed infants [66,67].…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Probiotic supplementation can positively affect anxiety and depressive symptoms: a systematic review of randomized controlled trials

et al. 2016

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“…Because the sample is easily acquired, repeated sampling over time or after repeated treatments is possible, such as in a crossover intervention study. With this method, conflation of host transcriptome profiles with changes in microbiome can be achieved (50, 186). …”

Section: Novel Techniques To Study Nutrient-gene Interaction In Colonmentioning

confidence: 99%

Nutrient-Gene Interaction in Colon Cancer, from the Membrane to Cellular Physiology

et al. 2016

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The International Agency for Research on Cancer recently released an assessment classifying red and processed meat as “carcinogenic to humans” on the basis of the positive association between increased consumption and risk for colorectal cancer. Diet, however, can also decrease the risk for colorectal cancer and be used as a chemopreventive strategy. Bioactive dietary molecules, such as n-3 polyunsaturated fatty acids, curcumin, and fermentable fiber, have been proposed to exert chemoprotective effects, and their molecular mechanisms have been the focus of research in the dietary/chemoprevention field. Using these bioactives as examples, this review surveys the proposed mechanisms by which they exert their effects, from the nucleus to the cellular membrane. In addition, we discuss emerging technologies involving the culturing of colonic organoids to study the physiological effects of dietary bioactives. Finally, we address future challenges to the field regarding the identification of additional molecular mechanisms and other bioactive dietary molecules that can be utilized in our fight to reduce the incidence of colorectal cancer.

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Noninvasive molecular fingerprinting of host–microbiome interactions in neonates

Cited by 34 publications

References 84 publications

miRNA-regulated gene expression differs in celiac disease patients according to the age of presentation

miRNA-regulated gene expression differs in celiac disease patients according to the age of presentation

Probiotic supplementation can positively affect anxiety and depressive symptoms: a systematic review of randomized controlled trials

Nutrient-Gene Interaction in Colon Cancer, from the Membrane to Cellular Physiology

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