Noninvasive mapping of regional response to segmental allergen challenge using magnetic resonance imaging and [F‐18]fluorodeoxyglucose positron emission tomography

Holmes, James H.; Sorkness, Ronald L.; Meibom, Sara K.; Sundaram, Senthilarasu; Perlman, Scott; Converse, Alexander K.; Pyzalski, Robert W.; Hahn, Andrew D.; Korosec, Frank R.; Grist, Thomas M.; Fain, Sean B.

doi:10.1002/mrm.20504

Cited by 11 publications

(11 citation statements)

References 29 publications

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“…These images reveal both changes in ventilation and airway caliber. The ability to resolve the airways of small animals by 3 He MRI is particularly important to the study of airways hyperreactivity and has historically been accomplished only by groups employing radial sampling, including Duke, Wisconsin (33), and Lyon (34,35). In this work, we have provided simple calculations to explain how using radial imaging and avoiding slice selection mitigates the effects of 3 He diffusion, and makes airway imaging in mice possible.…”

Section: Discussionmentioning

confidence: 99%

³He MRI in mouse models of asthma

Driehuys

Walker

Pollaro

et al. 2007

Magnetic Resonance in Med

114

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In the study of asthma, a vital role is played by mouse models, because knockout or transgenic methods can be used to alter disease pathways and identify therapeutic targets that affect lung function. Assessment of lung function in rodents by available methods is insensitive because these techniques lack regional specificity. A more sensitive method for evaluating lung function in human asthma patients uses hyperpolarized (HP) 3 He MRI before and after bronchoconstriction induced by methacholine (MCh). We now report the ability to perform such 3 He imaging of MCh response in mice, where voxels must be ϳ3000 times smaller than in humans and 3 He diffusion becomes an impediment to resolving the airways. We show three-dimensional (

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Section: Discussionmentioning

confidence: 99%

³He MRI in mouse models of asthma

Driehuys

Walker

Pollaro

et al. 2007

Magnetic Resonance in Med

114

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“…For example, 18 F-fluorodeoxyglucose, in conjunction with positron emission tomography (PET), is commonly used to assess increased glucose uptake, and therefore metabolism, as a surrogate of inflammatory cell activity [14]. Unfortunately, this technique has not been shown to be a useful assessment of airway inflammation in asthmatics [15], unless under conditions of a segmental allergen challenge [16,17], most likely because neutrophils, a cell-type with a debatable association to allergic asthma, use more glucose than other cell types when activated [18,19]. Magnetic resonance imaging has also been used to detect inflammatory changes [20][21][22] as well as functional disruption after allergen exposure [23] in experimental models of asthma.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of allergic lung inflammation by computed tomography in a rat model in vivo

Jobse

Johnson²,

Farncombe³

et al. 2009

European Respiratory Journal

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The ability of micro-computed tomography (CT) to noninvasively evaluate allergic pulmonary inflammation in an experimental model was investigated. In addition, two image segmentation methods and the value of respiratory gating were investigated in the context of this model.Brown Norway rats were exposed to one of four doses of house dust mite (HDM) extract (0, 0.15, 15 or 150 mg) delivered intratracheally every 24 h for 10 days. CT scanning was performed at baseline and after several longitudinal HDM exposures.Both thoracic-and lung-segmentation methods yielded similar results when standardisation practices were employed. While tissue histology correlated well with CT images, cell counts from bronchoalveolar lavage depicted greater inflammation than did density measures from CT images. Evidence from representative CT slices and transaxial density distribution indicated that inflammation was primarily associated with major airways and extended into the periphery from these focal points. Respiratory gating demonstrated that images of the inspiratory state provided greater contrast of inflammatory processes. Lastly, decreases in tidal volumes indicated significant mechanical respiratory changes in animals exposed to both 15 and 150 mg.In summary, CT image segmentation can extract pertinent data on in vivo allergic airway/lung inflammation. Furthermore, respiratory gating provides additional contrast and insight into these quantification practices.

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“…Early in the development of HP gas imaging, birdcage coils with good B 1 homogeneity were tuned for reception at both proton and He-3 resonance frequencies (61,62) to facilitate registration of MRI anatomy to ventilation. However, similar coils for human studies have lagged behind, in part due to the difficulty of covering the entire chest anatomy with birdcage designs.…”

Section: Rf Excitation and Signal Calibrationmentioning

confidence: 99%

Functional lung imaging using hyperpolarized gas MRI

Fain

Korosec

Holmes

et al. 2007

Magnetic Resonance Imaging

Self Cite

177

158

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The noninvasive assessment of lung function using imaging is increasingly of interest for the study of lung diseases, including chronic obstructive pulmonary disease (COPD) and asthma. Hyperpolarized gas MRI (HP MRI) has demonstrated the ability to detect changes in ventilation, perfusion, and lung microstructure that appear to be associated with both normal lung development and disease progression. The physical characteristics of HP gases and their application to MRI are presented with an emphasis on current applications. Clinical investigations using HP MRI to study asthma, COPD, cystic fibrosis, pediatric chronic lung disease, and lung transplant are reviewed. Recent advances in polarization, pulse sequence development for imaging with Xe-129, and prototype low magnetic field systems dedicated to lung imaging are highlighted as areas of future development for this rapidly evolving technology.

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Noninvasive mapping of regional response to segmental allergen challenge using magnetic resonance imaging and [F‐18]fluorodeoxyglucose positron emission tomography

Cited by 11 publications

References 29 publications

³He MRI in mouse models of asthma

³He MRI in mouse models of asthma

Evaluation of allergic lung inflammation by computed tomography in a rat model in vivo

Functional lung imaging using hyperpolarized gas MRI

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Noninvasive mapping of regional response to segmental allergen challenge using magnetic resonance imaging and [F‐18]fluorodeoxyglucose positron emission tomography

Cited by 11 publications

References 29 publications

3He MRI in mouse models of asthma

3He MRI in mouse models of asthma

Evaluation of allergic lung inflammation by computed tomography in a rat model in vivo

Functional lung imaging using hyperpolarized gas MRI

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About

³He MRI in mouse models of asthma

³He MRI in mouse models of asthma