Noninvasive Limb Ischemic Preconditioning Protects Against Myocardial I/R Injury in Rats

Li, Shujuan; Wu, Yongjian; Kang, Yi; Yin, Yongqiang; Gao, Weizhen; Liu, Yanxia; Jian-shi, Lou

doi:10.1016/j.jss.2009.03.017

Cited by 28 publications

(28 citation statements)

References 28 publications

(28 reference statements)

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“…However, most of these studies have investigated mechanisms at time points that are different from the ones used in the present study [during early reperfusion or in late reperfusion (Dow et al 2012)] and also using different animal species (Konstantinov et al 2005;Donato et al 2013;Brandenburger et al 2014), making difficult to establish a comparison. In agreement with our findings, Li et al (2010) showed that rIPC induces activation of Phosphatidylinositol 3-kinase and inhibition of Glycogen synthase kinase before myocardial ischaemia. However, the authors proposed that rIPC would induce release of a factor to the bloodstream, which activates the PI3K-Akt-GSK3β pathway at the cardiac level, suggesting a myocardial mechanism similar to IPC.…”

Section: Discussionsupporting

confidence: 93%

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Myocardial triggers involved in activation of remote ischaemic preconditioning

Donato

Goyeneche

Garcés

et al. 2016

Experimental Physiology

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New Findings r What is the central question of this study?Ischaemia-reperfusion of peripheral tissues protects the heart from subsequent myocardial ischaemia-reperfusion injury, a phenomenon referred to as remote ischaemic preconditioning (rIPC). This study evaluated the possible myocardial triggers of rIPC. r What is the main finding and its importance?Remote ischaemic preconditioning reduces infarct size through a vagal pathway and a mechanism involving phosphorylation of Akt and endothelial nitric oxide synthase, opening of mitochondrial ATP-dependent K + channels and an increase in mitochondrial H 2 O 2 production. All these phenomena occur before the myocardial ischaemia; hence, they could act as 'triggers' of rIPC.It has been proposed that remote ischaemic preconditioning (rIPC) activates a parasympathetic neural pathway. However, the myocardial intracellular mechanism of rIPC remains unclear. Here, we characterized some of the intracellular signals participating as rIPC triggers. Isolated rat hearts were subjected to 30 min of global ischaemia and 120 min of reperfusion (Non-rIPC group). In a second group, before the isolation of the heart, an rIPC protocol (three cycles of hindlimb ischaemia-reperfusion) was performed. The infarct size was measured with tetrazolium staining. Expression/phosphorylation of Akt and endothelial nitric oxide synthase (eNOS) and mitochondrial H 2 O 2 production were evaluated at the end of the rIPC protocol, before myocardial ischaemia-reperfusion. The rIPC significantly decreased the infarct size and induced Akt and eNOS phosphorylation. The protective effect on infarct size was abolished by cervical vagal section, l-NAME (an NO synthesis inhibitor) and 5-hydroxydecanoate

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Section: Discussionsupporting

confidence: 93%

“…From this point of view, several studies have evaluated the intracellular signalling pathway involved in the cardioprotection afforded by rIPC (Wolfrum et al 2002;Li et al 2010;Breivik et al 2011). However, those authors have not considered the timing sequence of signalling activation.…”

Section: Introductionmentioning

confidence: 99%

Myocardial triggers involved in activation of remote ischaemic preconditioning

Donato

Goyeneche

Garcés

et al. 2016

Experimental Physiology

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show abstract

“…Some studies recently suggested that NDLIP reduced myocardial ischemic injury [19], and we hypothesized that NDLIP had protective effects toward cerebral tissue during ischemia-reperfusion injury.…”

Section: Discussionmentioning

confidence: 98%

“…After 5 min, the aerocyst was deflated and the left hind limb was reperfused for 5 min. This inflation/deflation cycle was performed three times/d for 3 d [19].…”

Section: Induction Of Ndlipmentioning

confidence: 99%

“…We previously showed that in rats, noninvasive delayed limb ischemic preconditioning (NDLIP) induced by three cycles of 5-min occlusion and 5-min reperfusion of the left hind limb every day for 3 d confers the same cardioprotective effect as local IPC of the heart [19]. The aim of this project was to verify the hypotheses that NDLIP had neuroprotective effects during I/R injury.…”

Section: Introductionmentioning

confidence: 97%

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