Noninvasive Imaging of Aortic Atherosclerosis by Ultrasound Biomicroscopy in a Mouse Model

Zhang, Xiaoshan; Si, Huaijun; Wei, Wei; Duan, Shasha; Shi, Yilu; Yang, Ya

doi:10.7863/ultra.34.1.111

Cited by 11 publications

(9 citation statements)

References 12 publications

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“…Cine loop images were later analyzed using Vevo 2100 software. Intima-media thickness was calculated using previously validated approach(Zhang et al, 2015) i.e. from edge of echo toward the blood stream (intima) to other echo edge (adventitia) using ImageJ (National Institutes of Health).…”

Section: Star Methodsmentioning

confidence: 99%

Sympathetic Neuronal Activation Triggers Myeloid Progenitor Proliferation and Differentiation

et al. 2018

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There is a growing body of research on the neural control of immunity and inflammation. However, it is not known whether the nervous system can regulate the production of inflammatory myeloid cells from hematopoietic progenitor cells in disease conditions. Myeloid cell numbers in diabetic patients were strongly correlated with plasma concentrations of norepinephrine, suggesting the role of sympathetic neuronal activation in myeloid cell production. The spleens of diabetic patients and mice contained higher numbers of tyrosine hydroxylase (TH)-expressing leukocytes that produced catecholamines. Granulocyte macrophage progenitors (GMPs) expressed the β adrenergic receptor, a target of catecholamines. Ablation of splenic sympathetic neuronal signaling using surgical, chemical, and genetic approaches diminished GMP proliferation and myeloid cell development. Finally, mice lacking TH-producing leukocytes had reduced GMP proliferation, resulting in diminished myelopoiesis. Taken together, our study demonstrates that catecholamines produced by leukocytes and sympathetic nerve termini promote GMP proliferation and myeloid cell development.

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Section: Star Methodsmentioning

confidence: 99%

Sympathetic Neuronal Activation Triggers Myeloid Progenitor Proliferation and Differentiation

et al. 2018

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“…We suspected that the above results may be caused by abnormal lipid metabolism, which also suggested that alisol A may exert antiatherosclerotic effect by lowering plasma lipids. The non-invasive high-resolution small animal ultrasound system makes it possible to perform quantitative assessment in live mice, although the imaging plaque could be affected by the equipment and operator's experience (Zhang et al, 2015). The results of non-invasive small animal ultrasound examination in the mice three months after the drug administration demonstrated no statistical difference among the groups.…”

Section: Discussionmentioning

confidence: 98%

Alisol A Alleviates Arterial Plaque by Activating AMPK/SIRT1 Signaling Pathway in apoE-Deficient Mice

et al. 2020

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Alismatis Rhizoma (zexie), an herb used in traditional Chinese medicine, exhibits hypolipemic, anti-inflammation and anti-atherosclerotic activities. Alisol A is one of the main active ingredients in Alismatis Rhizoma extract. In this study, we investigate the role of alisol A in anti-atherosclerosis (AS). Our study demonstrated that alisol A can effectively inhibit the formation of arterial plaques and blocked the progression of AS in ApoE −/− mice fed with high-fat diet and significantly reduced the expression of inflammatory cytokins in aorta, including ICAM-1, IL-6, and MMP-9. In addition, we found that alisol A increased the expression of PPARa and PPARd proteins in HepG2 cells and in liver tissue from ApoE −/− mice. Alisol A activated the AMPK/SIRT1 signaling pathway and NF-kB inhibitor IkBa in HepG2 cells. Our results suggested that alisol A is a multi-targeted agent that exerts anti-atherosclerotic action by regulating lipid metabolism and inhibiting inflammatory cytokine production. Therefore, alisol could be a promising lead compound to develop drugs for the treatment of AS.

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“…Ultrasound bio-microscopy enables evaluation of plaque size and stenosis in vivo, noninvasively, and in real-time [ 17 , 23 – 25 ], which has been widely used to detect spontaneously formed plaques in the brachiocephalic [ 26 , 27 ], thoracic arteries [ 24 , 28 ], and the ascending aorta [ 23 ]. In this study, such technique is used to monitor the development of plaque of atherosclerosis and such technique will give the longitudinal study possible.…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-217 attenuates intima–media complex thickness of ascending aorta measured by ultrasound bio-microscopy and inhibits inflammation and lipid metabolism in atherosclerotic models of ApoE−/− mice

Liu

Song

et al. 2018

Lipids Health Dis

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BackgroundLittle investigation was done to test the efficiency of microRNA-217 (miR-217) on atherosclerosis in vivo.MethodsApoE−/− mice were used to construct atherosclerotic models and ultrasound bio-microscopy (UBM) was applied to detect the intima–media thickness (IMT) of the ascending aorta. The serum level of miR-217 and correlation with IMT was investigated. After miR-217 mimic administration, the IMT, inflammation, and lipid-associated molecules were assayed.ResultsThe serum level of miR-217 was reduced in ApoE−/− mice and showed a negative correlation with the IMT of the ascending aorta (r2 = 0.5899, p < 0.0001). miR-217 mimic administration attenuated IMT and down-regulated the level of serum triglyceride (TG), total cholesterol (TC), and low-density-lipoprotein cholesterol (LDL-C), while it could up-regulate high-density lipoprotein cholesterol (HDL-C). Inflammation relevant genes, such as F4/80, tumor necrosis factor (TNF)-α, interleukin (IL)-1, IL-6, and monocyte chemoattractant protein (MCP)-1, and lipid metabolism associated gene, such as LDL receptor, class A scavenger receptors (SR-A), scavenger receptor class B type I (SR-BI), CD36, ATP binding cassette subfamily A member 1 (ABCA1), and ATP binding cassette subfamily G member 1 (ABCG1) in the aorta were significantly down-regulated in miR-217 group when compared with atherosclerosis group.ConclusionmiR-217 could down-regulate IMT and modulate the inflammation and lipid metabolism process, which indicates that miR-217 could be a potential treatment option.

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Noninvasive Imaging of Aortic Atherosclerosis by Ultrasound Biomicroscopy in a Mouse Model

Abstract: Ultrasound biomicroscopy could be used for a noninvasive, accurate, and dynamic analysis of aortic atherosclerosis in LDL-R knockout mice.

Cited by 11 publications

References 12 publications

Sympathetic Neuronal Activation Triggers Myeloid Progenitor Proliferation and Differentiation

Sympathetic Neuronal Activation Triggers Myeloid Progenitor Proliferation and Differentiation

Alisol A Alleviates Arterial Plaque by Activating AMPK/SIRT1 Signaling Pathway in apoE-Deficient Mice

MicroRNA-217 attenuates intima–media complex thickness of ascending aorta measured by ultrasound bio-microscopy and inhibits inflammation and lipid metabolism in atherosclerotic models of ApoE−/− mice

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