Noninvasive High-Speed Photoacoustic Tomography of Cerebral Hemodynamics in Awake-Moving Rats

Tang, Jianbo; Xi, Lei; Zhou, Junli; Huang, Hua; Zhang, Tao; Carney, Paul R.; Jiang, Huabei

doi:10.1038/jcbfm.2015.138

Cited by 59 publications

(48 citation statements)

References 52 publications

(68 reference statements)

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“…Alternatively, non invasive PAI could be achieved by using an orally ingested contrast agent along with an external imaging probe (likely to consist of a pulsed excitation laser combined with a commer cially available ultrasound transducer similar to the devices reported in REF. 100 and REF. 101).…”

Section: Microbiota Changesmentioning

confidence: 92%

The potential role of optical biopsy in the study and diagnosis of environmental enteric dysfunction

Thompson

Hughes

Anastasova

et al. 2017

Nat Rev Gastroenterol Hepatol

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| Environmental enteric dysfunction (EED) is a disease of the small intestine affecting children and adults in low and middle income countries. Arising as a consequence of repeated infections, gut inflammation results in impaired intestinal absorptive and barrier function, leading to poor nutrient uptake and ultimately to stunting and other developmental limitations. Progress towards new biomarkers and interventions for EED is hampered by the practical and ethical difficulties of cross-validation with the gold standard of biopsy and histology. Optical biopsy techniques -which can provide minimally invasive or noninvasive alternatives to biopsy -could offer other routes to validation and could potentially be used as point-of-care tests among the general population. This Consensus Statement identifies and reviews the most promising candidate optical biopsy technologies for applications in EED, critically assesses them against criteria identified for successful deployment in developing world settings, and proposes further lines of enquiry. Importantly, many of the techniques discussed could also be adapted to monitor the impaired intestinal barrier in other settings such as IBD, autoimmune enteropathies, coeliac disease, graft-versus-host disease, small intestinal transplantation or critical care. Members of the board and experts met to discuss the horizon scan in London, UK, in November 2015 and San Francisco, USA, in February 2016. Literature searches were performed using Web of Science, as well as through the advice of the advisory board and experts. The results of the horizon scan are presented in this Consensus Statement, which entails a review of EED, a discussion of technologies deemed relevant to EED (based on literature searches and the opinions of the advisory board and experts), and a series of recommendations for the future application of the rele vant technologies to the study and diagnosis of EED. In the Recommendations section, each technology has been assessed according to its suitability for application to EED and this assessment includes an evaluation of the cost, invasiveness and appropriateness for developing countries of each device and/or technique. This assessment was performed in a qualitative manner based on currently available information (for example, the present cost and size of the devices) and the opinions of the authors as to feasible future develop ment. Technique suitability for use in developing countries was determined in a qualitative manner after consideration of the need for advanced infrastructure and high levels of training for either system use or data analysis. Probable deployment time scales have also been assigned to each technique based on the current states of development and the degree of valid ation work that will be required before application to EED. These timescales have been tentatively defined as short-term (suitable for deployment immediately or within ~1 year), mediumterm (suit able for deployment within ~1-3 years) and long-term (suitable for deployme...

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Section: Microbiota Changesmentioning

confidence: 92%

The potential role of optical biopsy in the study and diagnosis of environmental enteric dysfunction

Thompson

Hughes

Anastasova

et al. 2017

Nat Rev Gastroenterol Hepatol

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“…In addition, PAT has been increasingly used to study small-animal models of brain diseases, including stroke 80 , epilepsy 81 , and edema 60 . Recently, light-weight head-mounted PACT systems have been developed, an important step towards functional PA imaging of brains in free-moving animals 82 .…”

Section: Selected Applicationsmentioning

confidence: 99%

A practical guide to photoacoustic tomography in the life sciences

2016

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The life sciences can benefit greatly from imaging technologies that connect microscopic discoveries with macroscopic observations. Photoacoustic tomography (PAT), a highly sensitive modality for imaging rich optical absorption contrast over a wide range of spatial scales at high speed, is uniquely positioned for this need. In PAT, endogenous contrast reveals tissue’s anatomical, functional, metabolic, and histologic properties, and exogenous contrast provides molecular and cellular specificity. The spatial scale of PAT covers organelles, cells, tissues, organs, and small-animal organisms. Consequently, PAT is complementary to other imaging modalities in contrast mechanism, penetration, spatial resolution, and temporal resolution. We review the fundamentals of PAT and provide practical guidelines to the broad life science community for matching PAT systems with research needs. We also summarize the most promising biomedical applications of PAT, discuss related challenges, and envision its potential to lead to further breakthroughs.

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“…19 This model can generate focal seizures lasting up to hundreds of seconds with interictal periods of minutes for a duration of several hours. 15 In contrast to generalized models of epilepsy, which lead to a more widespread (global) activation in the entire brain, 13 the 4-AP model allows better characterization of the onset and spread of localized epileptic seizures. In our study, 0.6 μL of 25 mM 4-AP dissolved in artificial cerebrospinal fluid (ACSF) were injected intracranially by a robot injection system (Neurostar, Tuebingen, Germany) using a 15 to 25 μm diameter glass microcapillary.…”

Section: Epilepsy Modelmentioning

confidence: 99%

“…11 To this end, optoacoustic microscopy and tomography have been successfully employed to monitor brain hemodynamic responses due to electrical stimulation 9,12 and superficial seizure activity in mice. [13][14][15] Real-time optoacoustic imaging of two-dimensional (2-D) cross-sections through the whole mouse brain is further possible with systems based on arrays of cylindrically focused transducers. 16,17 However, efficient real-time volumetric optoacoustic visualization of epileptic seizures has not yet been achieved due to insufficient spatiotemporal resolution in three-dimensions (3-D) and lack of fast whole-brain imaging capacity.…”

Section: Introductionmentioning

confidence: 99%

Correlation between volumetric oxygenation responses and electrophysiology identifies deep thalamocortical activity during epileptic seizures

et al. 2016

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Abstract. Visualization of whole brain activity during epileptic seizures is essential for both fundamental research into the disease mechanisms and the development of efficient treatment strategies. It has been previously discussed that pathological synchronization originating from cortical areas may reinforce backpropagating signaling from the thalamic neurons, leading to massive seizures through enhancement of high frequency neural activity in the thalamocortical loop. However, the study of deep brain neural activity is challenging with the existing functional neuroimaging methods due to lack of adequate spatiotemporal resolution or otherwise insufficient penetration into subcortical areas. To investigate the role of thalamocortical activity during epileptic seizures, we developed a new functional neuroimaging framework based on spatiotemporal correlation of volumetric optoacoustic hemodynamic responses with the concurrent electroencephalogram recordings and anatomical brain landmarks. The method is shown to be capable of accurate three-dimensional mapping of the onset, spread, and termination of the epileptiform events in a 4-aminopyridine acute model of focal epilepsy. Our study is the first to demonstrate entirely noninvasive real-time visualization of synchronized epileptic foci in the whole mouse brain, including the neocortex and subcortical structures, thus opening new vistas in systematic studies toward the understanding of brain signaling and the origins of neurological disorders.

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Noninvasive High-Speed Photoacoustic Tomography of Cerebral Hemodynamics in Awake-Moving Rats

Cited by 59 publications

References 52 publications

The potential role of optical biopsy in the study and diagnosis of environmental enteric dysfunction

The potential role of optical biopsy in the study and diagnosis of environmental enteric dysfunction

A practical guide to photoacoustic tomography in the life sciences

Correlation between volumetric oxygenation responses and electrophysiology identifies deep thalamocortical activity during epileptic seizures

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