Noninvasive genetic assessment provides evidence of extensive gene flow and possible high movement ability in the African golden wolf

Karssene, Yamna; Chammem, Mohsen; Nowak, Carsten; Smet, Koen De; Castro, Diana; Eddine, Ahmed; Lopes, Susana; Muñoz‐Fuentes, Violeta; Cocchiararo, Berardino; Klees, Dick; Leer, Peter van der; Nouira, Saïd; Godinho, Raquel

doi:10.1016/j.mambio.2018.05.002

Cited by 11 publications

(11 citation statements)

References 52 publications

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“…Faecal DNA-based sampling to identify individuals and estimate the population size was implemented in e.g. Asian elephants Elephas maximus (Gray et al 2014), mountain gorillas Gorilla beringei beringei (Roy et al 2014), Indian rhinoceros Rhinoceros unicornis (Das et al 2015), Cabrera's voles Microtus cabrerae (Proença-Ferreira et al 2019), African golden wolves Canis anthus (Karssene et al 2018), kit foxes Vulpes macrotis mutica (Wilbert et al 2015) or birds Apteryx spp., Otis tarda, Tetrao urogallus (Idaghdour et al 2003, Perez et al 2011, Rosner et al 2014, Ramon-Laca et al 2018, Vallant et al 2018. Faecal DNA has been also used for identifying insects like Bombus spp.…”

Section: Dna Samplingmentioning

confidence: 99%

Towards more compassionate wildlife research through the 3Rs principles: moving from invasive to non-invasive methods

Zemanova

2020

Wildlife Biology

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Section: Dna Samplingmentioning

confidence: 99%

Towards more compassionate wildlife research through the 3Rs principles: moving from invasive to non-invasive methods

Zemanova

2020

Wildlife Biology

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“…A set of 47 microsatellite loci was amplified in five multiplex reactions for tissue samples following [24] and [25] (electronic supplementary material, table S3). For scat samples, we genotyped a subset of 13 microsatellites previously optimized in three pools following [26]. Four PCR replicas of each marker were accomplished per non-invasive sample.…”

Section: (C) Microsatellites Genotypingmentioning

confidence: 99%

“…Mitochondrial diversity was assessed using sequences generated in this study (n = 22), and then together with 46 sequences from Algeria and Tunisia retrieved from previous works [14,26]. Diversity indices were assessed using DnaSP 5 [27].…”

Section: (D) Diversity and Genetic Structurementioning

confidence: 99%

Demographic expansion of an African opportunistic carnivore during the Neolithic revolution

et al. 2020

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The diffusion of Neolithic technology together with the Holocene Climatic Optimum fostered the spread of human settlements and pastoral activities in North Africa, resulting in profound and enduring consequences for the dynamics of species, communities and landscapes. Here, we investigate the demographic history of the African wolf ( Canis lupaster ), a recently recognized canid species, to understand if demographic trends of this generalist and opportunistic carnivore reflect the increase in food availability that emerged after the arrival of the Neolithic economy in North Africa. We screened nuclear and mitochondrial DNA in samples collected throughout Algeria and Tunisia, and implemented coalescent approaches to estimate the variation of effective population sizes from present to ancestral time. We have found consistent evidence supporting the hypothesis that the African wolf population experienced a meaningful expansion concurring with a period of rapid population expansion of domesticates linked to the advent of agricultural practices.

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“…Since then it has been used in a large number of studies that evaluated species abundance (e.g. Piaggio et al 2016), genetic diversity (Epps et al 2005), gene flow (Kormann et al 2012;Karssene et al 2018), population size (Solberg et al 2006;Zarzoso-Lacoste et al 2018), phylogeography (Morin et al 1993) or hybridization (Anile et al 2014;Velli et al 2015).…”

Section: Introductionmentioning

confidence: 99%

Poor implementation of non-invasive sampling in wildlife genetics studies

Zemanova¹

2019

ReEco

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The Earth’s biodiversity is currently experiencing immense pressure from habitat loss, overexploitation, global climate change, and invasive species, which escalate the global extinction crisis. Comprehensive knowledge of the extent and impact of biodiversity loss is therefore critical for determining species vulnerability and prioritizing conservation goals. An integral part of wildlife conservation research and management is nowadays genetic sampling. Animal DNA has been traditionally obtained invasively, from blood or other tissues, however public concerns over animal welfare require that animals are affected as little as possible during research. One of the ways to minimize the impact on wildlife animal welfare is to use non-invasive genetic sampling. Even though non-invasive genetic sampling techniques have been developed for many animal species, it is not clear how often they are being implemented. Here, I present an overview of recently published articles on genetics in amphibians, birds, carnivores, molluscs and rodents, for which I examined whether they used a lethal, invasive or non-invasive DNA sampling technique. Disappointingly, only 22% of the identified relevant studies implemented the available non-invasive genetic sampling method. I conclude highlighting the need for better implementation of non-invasive DNA collection methods in wildlife research through raising awareness, increasing financial support, and introducing more stringent criteria for obtaining research permits.

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Noninvasive genetic assessment provides evidence of extensive gene flow and possible high movement ability in the African golden wolf

Cited by 11 publications

References 52 publications

Towards more compassionate wildlife research through the 3Rs principles: moving from invasive to non-invasive methods

Towards more compassionate wildlife research through the 3Rs principles: moving from invasive to non-invasive methods

Demographic expansion of an African opportunistic carnivore during the Neolithic revolution

Poor implementation of non-invasive sampling in wildlife genetics studies

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