Noninvasive evaluation of skeletal muscle mitochondrial capacity with near-infrared spectroscopy: correcting for blood volume changes

Ryan, Terence E.; Erickson, Melissa L.; Brizendine, Jared T.; Young, Hui-Ju; McCully, Kevin K.

doi:10.1152/japplphysiol.00319.2012

Cited by 170 publications

(288 citation statements)

References 38 publications

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“…6 Future studies should measure TSI trend during dynamic exercise of those with ATT between 7 and 15 mm and determine whether the influence of ATT can be removed by applying an ischemic calibration as has been shown previously for muscle oxygen consumption measurements previously. 55 …”

Section: Real World Applicationsmentioning

confidence: 99%

Performance comparison of the MOXY and PortaMon near-infrared spectroscopy muscle oximeters at rest and during exercise

2018

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Abstract. The purpose of the study was to compare muscle oxygenation as measured by two portable, wireless near-infrared spectroscopy (NIRS) devices under resting and dynamic conditions. A recently developed low-cost NIRS device (MOXY) was compared against an established PortaMon system that makes use of the spatially resolved spectroscopy algorithm. The influence of increasing external pressure on tissue oxygen saturation index (TSI) indicated that both devices are stable between 2 and 20 mmHg. However, above this pressure, MOXY reports declining TSI values. Analysis of adipose tissue thickness (ATT) and TSI shows a significant, nonlinear difference between devices at rest. The devices report similar TSI (%) values at a low ATT (<7 mm) (PortaMon minus MOXY difference is þ1.1 AE 2.8%) with the major subsequent change between the devices occurring between 7 and 10 mm; at ATT values >10 mm the difference remains constant (−14.7 AE 2.8%). The most likely explanation for this difference is the small source-detector separation (2.5 cm) in the MOXY resulting in lower tissue penetration into muscle in subjects with higher ATT. Interday test-retest reliability of resting TSI was evaluated on five separate occasions, with the PortaMon reporting a lower coefficient of variation (1.8% to 2.5% versus 5.7% to 6.2%). In studies on male subjects with low ATT, decreases in the TSI were strongly correlated during isometric exercise, arterial occlusion, and incremental arm crank exercise. However, the MOXY reports a greater dynamic range, particularly during ischemia induced by isometric contraction or occlusion (Δ74.3% versus Δ43.7%; hyperemia MAX-occlusion MIN). This study shows that in this subject group both MOXY and PortaMon produce physiologically credible TSI measures during rest and exercise. However, the absolute values obtained during exercise are generally not comparable between devices unless corrected by physiological calibration following an arterial occlusion. © The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Section: Real World Applicationsmentioning

confidence: 99%

Performance comparison of the MOXY and PortaMon near-infrared spectroscopy muscle oximeters at rest and during exercise

2018

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“…Another electrical stimulation of 30 s occurred and the recovery assessment of the protocol commenced. The recovery consisted of 24 short blood pressure cuff occlusions also at 230 mmHg and was repeated twice [3] (see Figure 2 for a diagram of the protocol). For the calf, the medium blood pressure cuff (SC12D cuff, D.E.…”

Section: Visitmentioning

confidence: 99%

“…A more recent application of NIRS is for the assessment of muscle oxygen consumption and uses muscle field stimulation to increase muscle metabolic activity and arterial occlusions to create recovery slopes for each occlusion [2]. The arterial occlusion slopes are fit to an exponential curve and a time constant is calculated as a measure of mitochondrial capacity [3]. Depending on the mitochondrial time constant, higher mitochondria capacity correlates to a lower time constant, while lower mitochondrial capacity relates to a higher time constant.…”

Section: Introductionmentioning

confidence: 99%

Reliability and Validity of Near-Infrared Spectroscopy Mitochondrial Capacity Measurement in Skeletal Muscle

Mantia

Neidert

Kluess

2018

JFMK

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Establish reliability of near-infrared spectroscopy (NIRS) mitochondrial capacity measurement and assess validity using heating and cooling interventions. We recruited 13 participants for four visits. Two visits consisted of a series of blood pressure occlusions proximal to the NIRS probe and electrical stimulation to the thigh, calf and forearm for reliability. Visits 3 and 4 required heating or cooling of the three muscles, using the protocol above. The between day reliability coefficient of variation (CV) in the forearm was 3% and time constant (TC) average was 41.9 ± 9.2 seconds (s). The within day CV in the forearm = 4.5%, the calf = 5.5% and the thigh = 4.6% (n = 13). Within day reliability for the cold condition in the forearm = 5.9%, calf = 4.3% and thigh = 4.4% and within day for the hot condition in the forearm = 6.4%, calf = 5.3% and thigh = 4.6%. The cold condition increased TC in the forearm, but decreased TC with heat (p < 0.05). The thigh decreased mitochondrial capacity with heat (p < 0.05), but cold was no different from control. These results suggest NIRS mitochondrial capacity has good within and between day reliability and temperature changes the mitochondrial capacity time constant.

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“…Not only can NIRS measure the adequacy of tissue oxygen delivery, it can also be used to assess mitochondrial oxygen consumption and redox status (25). In an animal model of severe hemorrhagic shock, Rhee et al noted that despite adequate resuscitation, as measured by the normalization of blood pressure, cardiac output and tissue oxygen delivery, mitochondrial cytochrome a,a3 oxidation remained significantly decreased in splanchnic and skeletal muscle beds (26).…”

Section: Discussionmentioning

confidence: 99%

Can Peripheral Blood Mononuclear Cells Be Used as a Proxy for Mitochondrial Dysfunction in Vital Organs During Hemorrhagic Shock and Resuscitation?

Karamercan¹,

Weiss²,

Villarroel

et al. 2013

Shock

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INTRODUCTION Although mitochondrial dysfunction is thought to contribute to the development of post-traumatic organ failure, current techniques to assess mitochondrial function in tissues are invasive and clinically impractical. We hypothesized that mitochondrial function in peripheral blood mononuclear cells (PBMCs) would reflect cellular respiration in other organs during hemorrhagic shock and resuscitation (HS&R). METHODS Using a fixed pressure HS model, Long Evan’s rats were bled to a mean arterial pressure (MAP) of 40 mmHg. When blood pressure could no longer be sustained without intermittent fluid infusion (Decompensated HS), Lactated Ringer’s (LR) was incrementally infused to maintain the MAP at 40 mmHg until 40% of the shed blood volume was returned (Severe HS). Animals were then resuscitated with 4X total shed volume in LR over 60 minutes (Resuscitation). Control animals underwent the same surgical procedures, but were not hemorrhaged. Animals were randomized to Control (n=6), Decompensated HS (n=6), Severe HS (n=6) or Resuscitation (n=6) groups. Kidney, liver, and heart tissues as well as PBMC’s were harvested from animals in each group to measure mitochondrial oxygen consumption using high resolution respirometry. Flow cytometry was used to assess mitochondrial membrane potential (Ψm) in PBMCs. One-way ANOVA and Pearson correlations were performed. RESULTS Mitochondrial oxygen consumption decreased in all tissues, including PBMC’s, following Decompensated HS, Severe HS, and Resuscitation. However, the degree of impairment varied significantly across tissues during HS&R. Of the tissues investigated, PBMC mitochondrial oxygen consumption and Ψm provided the closest correlation to kidney mitochondrial function during HS (complex I: r =0.65; complex II: r=0.65; complex IV: r=0.52; p<0.05). This association, however, disappeared with resuscitation. A weaker association between PBMC and heart mitochondrial function was observed but no association was noted between PBMC and liver mitochondrial function. CONCLUSION All tissues including PBMC’s demonstrated significant mitochondrial dysfunction following HS&R. Although PBMC and kidney mitochondrial function correlated well during hemorrhagic shock, the variability in mitochondrial response across tissues over the spectrum of hemorrhagic shock and resuscitation limits the usefulness of using PBMC’s as a proxy for tissue-specific cellular respiration.

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Noninvasive evaluation of skeletal muscle mitochondrial capacity with near-infrared spectroscopy: correcting for blood volume changes

Cited by 170 publications

References 38 publications

Performance comparison of the MOXY and PortaMon near-infrared spectroscopy muscle oximeters at rest and during exercise

Performance comparison of the MOXY and PortaMon near-infrared spectroscopy muscle oximeters at rest and during exercise

Reliability and Validity of Near-Infrared Spectroscopy Mitochondrial Capacity Measurement in Skeletal Muscle

Can Peripheral Blood Mononuclear Cells Be Used as a Proxy for Mitochondrial Dysfunction in Vital Organs During Hemorrhagic Shock and Resuscitation?

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