Noninvasive evaluation of in vivo free radical reactions catalyzed by iron using in vivo ESR spectroscopy

Phumala, Noppawan; Ide, Tatsuo; Utsumi, Hideo

doi:10.1016/s0891-5849(98)00314-1

Cited by 72 publications

(46 citation statements)

References 47 publications

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“…We concluded that intracellular iron mainly contributed the enhanced rate of signal decay in iron overloaded mice. 15,30,31) DF is a membrane impermeable iron chelator and it does not effectively chelated iron from intracellular pool. Therefore, the significant effects of DF were not observed in this experiment.…”

Section: Discussionmentioning

confidence: 99%

“…Thus, the rate of ESR signal decay of nitroxyl spin probes have been used to monitor free radical production and redox status in various animal models, including tumor inoculated in mouse footpads, 8) indomethacin-induced gastric ulcers, 9) rheumatoid arthritis, 10) hypertension, 11) cerebral ischemia-reperfusion, 12) lung injury caused by diesel exhaust particles, 13) and radiationinduced oxidative injury. 14) Phumala et al 15) have demonstrated in an acute iron overload murine model that free radical production is dependent on the availability of "chelatable iron" present transiently following a single injection of ferric citrate (0.2 µmol/g body weight). Moreover, the efficacy of an iron chelator, deferoxamine, to inhibit the enhancement of ESR signal decay is related also to the level of "chelatable iron" in the blood compartment.…”

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confidence: 99%

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Hepatic Reduction of Carbamoyl-PROXYL in Ferric Nitrilotriacetate Induced Iron Overloaded Mice: An <i>in Vivo</i> ESR Study

Morales

Yamaguchi

Murakami

et al. 2012

Biological & Pharmaceutical Bulletin

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Reduction of a nitroxyl radical, carbamoyl-PROXYL in association of free radical production and hepatic glutathione (GSH) was investigated in iron overloaded mice using an in vivo L-band electron spin resonance (ESR) spectrometer. Significant increases in hepatic iron, lipid peroxidation and decrease in hepatic GSH were observed in mice intraperitoneally (i.p.) administrated with ferric nitrilotriacetate (Fe(III)-NTA, a total 45 µmol/mouse over a period of 3 weeks). Free radical production in iron overloaded mice was evidenced by significantly enhanced rate constant of ESR signal decay of carbamoyl-PROXYL, which was slightly reduced by treatment with iron chelator, deferoxamine. Moreover, the rate constant of ESR signal decay was negatively correlated with hepatic GSH level (r 0.586, p<0.001). On the other hand, hepatic GSH-depletion (>80%) in mice through daily i.p. injection and drinking water supplementation of L-buthionine-[S,R]-sulfoximine (BSO) significantly retarded ESR signal decay, while there were no changes in serum aspartate aminotransferase and liver thiobarbituric acid-reactive substances levels. In conclusion, GSH plays two distinguish roles on ESR signal decay of carbamoyl-PROXYL, as an antioxidant and as a reducing agent, dependently on its concentration. Therefore, it should be taken into account in the interpretation of free radical production in each specific experimental setting.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Hepatic Reduction of Carbamoyl-PROXYL in Ferric Nitrilotriacetate Induced Iron Overloaded Mice: An <i>in Vivo</i> ESR Study

Morales

Yamaguchi

Murakami

et al. 2012

Biological & Pharmaceutical Bulletin

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“…Iron impurities in fossil fuels convert on combustion to magnetic iron oxides, i.e., magnetite, hematite or a mixture of both. Due to its combustion origin, magnetic PM is not only dangerous in itself (Phumala et al, 1999;Garcon et al, 2000;, but is also associated with other hazardous pollutants which are injected into the atmosphere during combustion (Morris et al, 1995;Muxworthy et al, 2001). PM in vehicle exhaust is dominated by particles < 10 μm, i.e., PM 10 (Kim et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

Magnetic signature of daily sampled urban atmospheric particles

Muxworthy

Matzka

Dávila

et al. 2003

Atmospheric Environment

103

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The magnetic signature of two sets of daily-sampled particulate matter (PM) collected in Munich, Germany, were examined and compared to variations in other pollution data and meteorological data using principal component analysis. The magnetic signature arising from the magnetic minerals in the PM, was examined using a fast and highly sensitive magnetic remanence measurement. The longest data set studied was 160 days, significantly longer than that of similar magnetic PM studies improving the statistical robustness. It was found that the variations in the mass dependent magnetic parameters displayed a complicated relationship governed by both the meteorological conditions and the PM loading rate, whereas mineralogy/grain-size dependent magnetic parameters displayed little variation. A six-fold increase in the number of vehicles passing the sampling locations only doubled the magnetic remanence of the samples, suggesting that the measured magnetic signature is in addition strongly influenced by dispersion rates. At both localities the saturation isothermal remanent magnetisation (SIRM) was found to be strongly correlated with the PM mass, and it is suggested that measuring SIRM as a proxy for PM monitoring is a viable alternative to magnetic susceptibility when the samples are magnetically too weak. The signal was found to be dominated by magnetite-like grains less than 100 nm in diameter which is thought to be derived primarily from vehicles. Such small grains are known to be particularly dangerous to humans. There was also evidence to suggest from magnetic stability parameters that the magnetite-like grains were covered with an oxidised rim. The concentration of magnetic particulate matter was in the range of 0.3-0.5% by mass.

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“…The in vivo EPR signal decay rates of the spin probes are enhanced by reactive oxygen species (Utsumi et al, 1993;Sano et al, 1998;Phumala et al, 1999;Han et al, 2001), such as hydroxyl radical and superoxide, which reduce nitroxyl radical in presence of H atom donor, such as NAD(P)H or glutathione (Samuni et al, 1988;Krishna et al, 1992;Samuni et al, 2002;Takeshita et al, 2002). In contrast, the decay rates are decreased due to reoxidation of the hydroxylamine to the nitroxyl radical under the oxidative atmosphere such as exposure to hyperoxia or generation of hydrogen peroxide (Quaresima et al, 1993;Matsumoto et al, 2000).…”

mentioning

confidence: 99%

Novel Pharmacokinetic Measurement Using Electron Paramagnetic Resonance Spectroscopy and Simulation of in Vivo Decay of Various Nitroxyl Spin Probes in Mouse Blood

Matsumoto

Krishna²,

Mitchell³

2004

J Pharmacol Exp Ther

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A novel approach to measure the time course of paramagnetic spin probe concentration in the circulating blood of a living mouse using X-band (9.4 GHz) electron paramagnetic resonance spectrometer is described. Using this technique, the pharmacokinetics of several nitroxyl spin probes was examined. The decay profiles were also independently simulated using pharmacokinetic properties as well as redox-mediated factors responsible in converting the nitroxyl radicals to the corresponding hydroxylamines. Finally, suitability of nitroxyl radicals as the probes of in vivo redox status and for radioprotection was described. The studies indicate that the six-member piperidine nitroxyls are suitable for estimating redox status in the circulation, whereas the five-member pyrrolidine nitroxyl radicals are suited for tissue redox status determination. For selective protection against radiation of normal tissues rather than cancer/tumor, efficient reoxidation of the hydroxylamine in normal tissue is preferable. Simulation results showed that for carbamoyl-PROXYL, only administration of the radical form might give radioprotection and not the hydroxylamine. However, the hydroxylamine form of TEMPOL, i.e., TEMPOL-H, may give similar radioprotection as the radical form due to efficient reoxidation in vivo.Nitroxyl radicals have been widely used as spin probes for low-frequency in vivo EPR experiments to estimate the biological redox status in living experimental animals (Berliner and Wan, 1989;Ilangovan et al., 2002;Kuppusamy et al., 2002;Yamada et al., 2002;Kasazaki et al., 2003). When a nitroxyl spin probe is administered to a living animal, in vivo EPR signal intensities of the probe show characteristic signal intensity profiles as a function of time in the animal, depending on the organ investigated. Generally, in vivo EPR signal decay rates are obtained based on a suitable region of its decay curve to estimate redox status in the animal .Several nitroxyl radicals have been used in studies that exhibit EPR signal decay profiles, depending on the spin probe used. The decay constant of a spin probe depends on the route of administration (i.e., intravenous, intraperitoneal) and methodology of the analysis (i.e., one-compartment model, two-compartment model) (Kocherginsky and Swartz, 1995). Moreover, species, strains, and gender of the experimental animal was found to affect the EPR signal decay profiles (Kocherginsky and Swartz, 1995;Matsumoto et al., 2000).The following general observations can be summarized from earlier studies. The in vivo EPR signal decay rates of the spin probes are enhanced by reactive oxygen species (Utsumi et al., 1993;Sano et al., 1998;Phumala et al., 1999;Han et al., 2001), such as hydroxyl radical and superoxide, which reduce nitroxyl radical in presence of H atom donor, such as NAD(P)H or glutathione (Samuni et al., 1988;Krishna et al., 1992;Samuni et al., 2002;Takeshita et al., 2002). In contrast, the decay rates are decreased due to reoxidation of the hydroxylamine to the nitroxyl radical un...

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Noninvasive evaluation of in vivo free radical reactions catalyzed by iron using in vivo ESR spectroscopy

Cited by 72 publications

References 47 publications

Hepatic Reduction of Carbamoyl-PROXYL in Ferric Nitrilotriacetate Induced Iron Overloaded Mice: An <i>in Vivo</i> ESR Study

Hepatic Reduction of Carbamoyl-PROXYL in Ferric Nitrilotriacetate Induced Iron Overloaded Mice: An <i>in Vivo</i> ESR Study

Magnetic signature of daily sampled urban atmospheric particles

Novel Pharmacokinetic Measurement Using Electron Paramagnetic Resonance Spectroscopy and Simulation of in Vivo Decay of Various Nitroxyl Spin Probes in Mouse Blood

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