Noninvasive estimation of chronological and photoinduced skin damage using Raman spectroscopy and principal component analysis

Abstract

In this work a relationship was found between histological properties of photoaged skin and noninvasive measurements based on Raman and principal components analysis (PCA). These relationships can be used to assess noninvasively the photoinduced damage and chronological characteristics of skin.

Evaluation and assessment of photoaging

“…A fourth study used Raman spectroscopy to correlate severity of clinical visual photoaging and histological properties of photoaged skin, noting changes in collagen and amount of solar elastosis [46].…”

In vivo confocal Raman spectroscopy for intrinsic aging and photoaging assessment

“…From the Raman spectroscopy studies that explore the dermis, few have focused on skin aging [7][8][9][10][11]. Gniadecka et al [8] used an ex vivo FT-Raman spectroscopy system to study intrinsic aging and photoaging.…”

“…Gonza'lez et al [9] first attempted to compare Raman spectroscopy findings and skin photoaging histology. The percentage of elastic fibres in photoaged skin correlated with the second component (PC2) of Principal Component Analysis (PCA) of the in vivo Raman spectra obtained from the same sites.…”

Raman characterization of human skin aging

“…[16][17][18] This is a very suitable technique for determining molecular and structural modifications occurring during biological process such as skin aging. 10,14,15,19,20 Gniadecka et al studied the water and protein structure in photo aged and chronically aged skin. They observed a decrease in amide III band intensity in the photo aged skin showing a change in the protein conformational structure.…”