Noninvasive detection and prediction of bladder cancer by fluorescence in situ hybridization analysis of exfoliated urothelial cells in voided urine

Ishiwata, Sho; Takahashi, Satoru; Homma, Yukio; Tanaka, Yoshinori; Kameyama, Shuji; Hosaka, Yoko; Kitamura, Tadaichi

doi:10.1016/s0090-4295(00)01074-8

Cited by 34 publications

(17 citation statements)

References 17 publications

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“…However, to our knowledge, none has demonstrated sufficient accuracy. 4,9,10 Similar to most malignancies, bladder cancer is a genetic disease involving many genetic changes. 7,11,12 Thus, methods to detect abnormal nuclear DNA content or chromosomal alterations appear to be promising, highly sensitive and specific diagnostic tools.…”

Section: Resultsmentioning

confidence: 99%

“…3,10,13 Alteration of a variety of chromosomes, including chromosomes 9, 17, 7, 11, 1, and others, have been reported as well. 9,14 Fluorescence in situ hybridization (FISH) can be used to identify cells in the urine that have chromosomal abnormalities consistent with a diagnosis of urothelial carcinoma. The UroVysion multicolor-FISH test (Vysis, Abbott Laboratories, Des Plaines, Ill), containing probes to the centromeres of chromosomes 3, 7, and 17 and to the 9p21 locus, was approved by the U.S. Food and Drug Administration (FDA) for use in detecting recurrent bladder cancer.…”

Section: Resultsmentioning

confidence: 99%

“…4,5,9,10 However, regardless of its high sensitivity, FISH depends on the presence of adequate numbers of exfoliated abnormal cells in the urine specimen. Therefore, insufficient urine volume, low tumor burden, or tumor cells that do not exfoliate will preclude the fulfillment of positivity criteria.…”

Section: Discussionmentioning

confidence: 99%

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Combined morphologic and fluorescence in situ hybridization analysis of voided urine samples for the detection and follow-up of bladder cancer in patients with benign urine cytology

Daniely

Rona

Kaplan³

et al. 2007

Cancer

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To meet the needs for polymer particulates for the flooding of oil fields, a composite crosslinked copolymer of hydrophilic monomers [acrylamide (AM) and acrylic acid (AA)] and a hydrophobic monomer [methyl methacrylate (MMA)] was synthesized. In this study, we first added a rare‐earth thermal stabilizer and inorganic montmorillonite (MMT) clay to the copolymer; this showed that the composite crosslinked copolymer exhibited good water‐swelling performance at 200°C, with gel static grade of 5, a toughness index of 1.1170, and a salinity limit of 250,000 mg/L. It also had better injection abilities. The AM/AA/MMA ratio was 4 : 1 : 0.03 w/w, the crosslinker additive content was 0.03 wt %, the thermal stabilizer additive content was 5 wt %, and the clay (MMT) additive content was 13 wt %. © 2006 Wiley Periodicals, Inc. J Appl Polym Sci 100: 4429–4433, 2006

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Combined morphologic and fluorescence in situ hybridization analysis of voided urine samples for the detection and follow-up of bladder cancer in patients with benign urine cytology

Daniely

Rona

Kaplan³

et al. 2007

Cancer

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“…TCC of the renal pelvis and ureter are relatively rare. The reported incidence in patients with previous urinary bladder TCC is only 2-5% [19] . The majority of these tumours manifest as haematuria and flank pain, symptoms which would require evaluation by radiological imaging.…”

Section: Discussionmentioning

confidence: 99%

Urine Cytology in the Evaluation of Urological Malignancy Revisited: Is It Still Necessary?

Falebita¹,

Lee

Sweeney³

2010

Urol Int

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Objective: We aim to determine if urine cytology was still necessary as a routine part of the evaluation for the presence of urological malignancy and to evaluate its cost effectiveness. Methods: Urine cytology reports over a 6-year period (2000–2005) were retrieved from our institution’s pathology department database. Patients with urine cytology positive for malignant cells were identified. We retrospectively reviewed the charts of these patients for age, sex, flexible cystoscopy and radiological imaging results. The cost of urine cytology was retrieved from the pathology department. Results: There were a total of 2,568 urine cytological examinations. Of these, 25 were positive for malignant cells. There were 19 male (76%) and 6 female (24%) patients with a mean age of 72 years (range: 49–97). In 21 patients with positive cytology, a bladder tumor was identified at flexible cystoscopy and/or imaging studies. For a positive cytology yield of 1%, EUR 210,000 was spent. Conclusions: Routine urine cytology was not cost effective and did not add to the diagnostic yield beyond cystoscopy and diagnostic imaging. It may be omitted in the initial evaluation of urological malignancy.

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“…Like other solid tumors, bladder cancer cells are characterized by a high frequency of chromosome abnormalities and bladder cancer cells in particular readily exfoliate into urine. Recent studies (Junker et al, 1999;Ishiwata et al, 2001) have demonstrated that the IFISH analysis for specific abnormalities may be useful to screen urine or bladder washings for early tumor detection or recurrence. This approach may be particularly useful because routine cytology analysis shows low sensitivity for bladder cancer.…”

Section: Applicationsmentioning

confidence: 99%

Interphase FISH as a new tool in tumor pathology

Tibiletti¹

2007

Cytogenet Genome Res

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Interphase FISH (IFISH) analysis is an intriguing molecular cytogenetic approach to study chromosome abnormalities in cancer. IFISH is a high sensitivity technique because of its ability to identify aberrations on a cell-to-cell level. The possibility to perform IFISH on different types of nuclei obtained both from fresh and archived samples, makes this technique an advantageous method to identify specific chromosome aberrations in cancer and correlate them to prognosis and therapy. The aim of this review is to outline the technical aspects, the sensitivity and specificity and the current strategies for employment of IFISH in tumor pathology, and to discuss the enormous range of novel applications.

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Noninvasive detection and prediction of bladder cancer by fluorescence in situ hybridization analysis of exfoliated urothelial cells in voided urine

Cited by 34 publications

References 17 publications

Combined morphologic and fluorescence in situ hybridization analysis of voided urine samples for the detection and follow-up of bladder cancer in patients with benign urine cytology

Combined morphologic and fluorescence in situ hybridization analysis of voided urine samples for the detection and follow-up of bladder cancer in patients with benign urine cytology

Urine Cytology in the Evaluation of Urological Malignancy Revisited: Is It Still Necessary?

Interphase FISH as a new tool in tumor pathology

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