Noninvasive analysis of skin proteins in healthy Chinese subjects using an Orbitrap Fusion Tribrid mass spectrometer

Abstract

Objective The objective of this study was to perform noninvasive analysis of skin proteins in a healthy Chinese population using label‐free nanoflow liquid chromatography‐mass spectrometry (nLC‐MS). Materials and Methods Five consecutive tape strippings were obtained from the volar forearm skin of healthy Chinese subjects. Proteins were extracted, and trypsin‐digested peptides were analyzed by a nanochromatography instrument coupled to an Orbitrap Fusion Tribrid mass spectrometer. Data‐dependent acquisition allowed protein identification, which was performed by using Proteome Discoverer software (v2.2). Results In this study, we identified 80 common proteins that were expressed in the skin of healthy Chinese volunteers and divided these proteins into 16 categories, including keratins, cornified envelope proteins, and enzymes associated with substance metabolism. These proteins were closely associated with multiple functions of the skin barrier. Conclusion This study provides a noninvasive method to analyze healthy human epidermal proteins, which are closely associated with the skin barrier. In addition, this study provides a reference for further studies on the application of proteomic technologies to investigate the role of human epidermal proteins in health and disease.

Comparative Proteomics Combined with Morphophysiological Analysis Revealed Chilling Response Patterns in Two Contrasting Maize Genotypes

“…Digested peptides were fractionated using an Ultimate 3000 system (Thermo Fisher Scientific, Waltham, MA, USA) [57]. Peptide fractions were analysed by online nanospray LC-MS/MS on an Orbitrap Fusion coupled to an EASY-nano-LC system (Thermo Scientific, Waltham, MA, USA) [58]. Proteome Discoverer software 2.0 (Thermo Fisher, Shanghai, China) [59] was used to process the raw MS/MS data obtained.…”

Towards Precision Dermatology: Emerging Role of Proteomic Analysis of the Skin

“…As mentioned, its complexity results in difficulties in separating and homogenizing the tissue for mass spectrometric analyses, and the dominance of structural proteins further complicates the process. Consequently, until recently, prior studies have only identified a relatively small fraction of the expressed skin proteome [21,22,33,[35][36][37][38][39]. For example, Mikesh et al [33] analyzed the composition in three skin layers from two anatomical regions, resulting in characterization of about 200 proteins and providing insight into varying protein composition depending on skin layer and skin region.…”

Application of Topical Sandalore® Increases Epidermal Dermcidin Synthesis in Organ-Cultured Human Skin ex vivo

“…Indeed, AD patients with reduced content of DCD suffer from recurrent Staphylococcus aureus infections [21]. Although proteolytically cleaved forms of DCD have been characterized as major AMPs in sweat [22], the uncleaved form is also reportedly detectable in the upper epidermis of healthy individuals [23]. DCD has specific antimicrobial activity against pathogenic species of Staphylococcus, Escherichia, Pseudomonas, Candida, and Listeria spp.…”