Nonhomologous RNA Recombination in a Cell-Free System: Evidence for a Transesterification Mechanism Guided by Secondary Structure

Chetverin, Alexander B.; Chetverina, Helena V.; Demidenko, Alexander A.; Ugarov, Victor I.

doi:10.1016/s0092-8674(00)81890-5

Cited by 145 publications

(160 citation statements)

References 39 publications

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“…This result represent an unique example of the utilization of 2 ,5 -linkage, produced in the self-ligation reaction from 2 ,3 -cyclic phosphate. Thus, it is tempting to speculate that similar ligation reactions with formation of 2 ,5 -linkages could proceed in other molecular systems, containg RNA molecules, providing not only stabilization, but a creation of new RNA molecules, as it was proposed by Chetverin et al (1997).…”

Section: Catalysis Of Ligation Reaction By Divalent Metal Ionsmentioning

confidence: 86%

The nonenzymatic template-directed ligation of oligonucleotides

et al. 2006

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Abstract. The nonenzymatic template-directed ligation of oligonucleotides containing 2 ,3 -cyclic phosphate was investigated in the presence of divalent cations. Ligation of the oligonucleotides readily occurred in the presence of Mg 2+ , Mn 2+ , Co 2+ , Zn 2+ , Pb 2+ . Efficacy of the metal ion catalysts inversely correlated with pKa values of the metal-bound water molecules. The intermolecular transesterification reaction yielded at least 95% of 2 ,5 -phosphodiester bonds independently on the nature of the metal ion. Relatively high reaction yields (up to 15%) suggest, that RNA fragmentation to oligonucleotides with 2 ,3 -cyclic phosphates, followed by reactions of those oligonucleotides could provide a source of new RNA molecules under prebiotic conditions.

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Section: Catalysis Of Ligation Reaction By Divalent Metal Ionsmentioning

confidence: 86%

The nonenzymatic template-directed ligation of oligonucleotides

et al. 2006

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“…RNA provides, for the time being, the only plausible solution to self-generation, as allowed by self-polymerization and by a variety of self-catalytic processes. Pioneering reports of RNA recombination have appeared [7][8][9][10][11][12]. With the possible exception of the systems described in [13][14][15], the reactions reported so far are not simple and robust enough to be of likely prebiotic relevance.…”

Section: Introductionmentioning

confidence: 99%

Ribozyme Activity of RNA Nonenzymatically Polymerized from 3′,5′-Cyclic GMP

Pino

Costanzo

Giorgi

et al. 2013

Entropy

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3′,5′-Cyclic GMP spontaneously nonenzymatically polymerizes in a base-catalyzed reaction affording G oligonucleotides. When reacted with fully or partially sequence-complementary RNA (oligo C), the abiotically generated oligo G RNA displays a typical ribozyme activity consisting of terminal ligation accompanied by cleavage of an internal phosphate site of the donor oligonucleotide stem upon attack of the acceptor 3′ terminal OH. This reaction is dubbed Ligation following Intermolecular Cleavage (LIC). In a prebiotic perspective, the ability of oligo G polynucleotides to react with other sequences outlines a simple and possible evolutionary scenario based on the autocatalytic properties of RNA.

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“…In one study (Chetverin et al 1997), overlapping 5Ј-and 3Ј-fragments were prepared from the small RNA species that, when intact, were replicable by the Q␤ phage replicase. In a cell-free system containing Q␤ replicase and rNTPs, these fragments were converted into species replicable by the phage enzyme as a result of nonhomologous recombination, which was dependent on the presence of the 3Ј-OH group on the 5Ј-fragment.…”

Section: Introductionmentioning

confidence: 99%

Nonreplicative homologous RNA recombination: Promiscuous joining of RNA pieces?

Gmyl¹,

Korshenko²,

Belousov³

et al. 2003

RNA

104

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Biologically important joining of RNA pieces in cells, as exemplified by splicing and some classes of RNA editing, is posttranscriptional, whereas in RNA viruses it is generally believed to occur during viral RNA polymerase-dependent RNA synthesis. Here, we demonstrate the assembly of precise genome of an RNA virus (poliovirus) from its cotransfected fragments, which does not require specific RNA sequences, takes place before generation of the viral RNA polymerase, and occurs in different ways: Apparently unrestricted ligation of the terminal nucleotides, joining of any one of the two entire fragments with the relevant internal nucleotide of its partner, or internal crossovers within the overlapping sequence. Incorporation of the entire 5 or 3 partners into the recombinant RNA is activated by the presence of terminal 3-phosphate and 5-OH, respectively. Such postreplicative reactions, fundamentally differing from the known site-specific and structurally demanding cellular RNA rearrangements, might contribute to the origin and evolution of RNA viruses and could generate new RNA species during all stages of biological evolution.

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Nonhomologous RNA Recombination in a Cell-Free System: Evidence for a Transesterification Mechanism Guided by Secondary Structure

Cited by 145 publications

References 39 publications

The nonenzymatic template-directed ligation of oligonucleotides

The nonenzymatic template-directed ligation of oligonucleotides

Ribozyme Activity of RNA Nonenzymatically Polymerized from 3′,5′-Cyclic GMP

Nonreplicative homologous RNA recombination: Promiscuous joining of RNA pieces?

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