Nongonococcal Urethritis Associated With Human Strains of "T" Mycoplasmas

Shepard, Maurice C.

doi:10.1001/jama.1970.03170080023005

Cited by 49 publications

(3 citation statements)

References 25 publications

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“…Tetracycline or erythromycin controlled infection. 221 Mycoplasma fermentans often is present in joints afflicted by rheumatoid arthritis. The microbic membrane inhibited leucocytic migration in twenty-nine of forty-three patients, but not in osteoarthritis or in normal persons.…”

Section: Miscellaneous Viral Studiesmentioning

confidence: 99%

Infectious diseases: annual review of significant publications

Reimann

1971

Postgraduate Medical Journal

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Section: Miscellaneous Viral Studiesmentioning

confidence: 99%

Infectious diseases: annual review of significant publications

Reimann

1971

Postgraduate Medical Journal

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“…The reported involvement of Ureaplasma urealyticum (T-strain mycoplasma) in nonspecific urethritis (11), reproductive failure (6), and infertility (5) has led to increased demand for laboratory identification of these organisms. However, few laboratories can perform these specialized identification techniques, and specimens must frequently be transported some distance for testing.…”

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confidence: 99%

Evaluation of preservation methods and solid media suitable for recovery of Ureaplasma urealyticum from transported urine specimens

Hipp¹,

Rockwood²,

Gaafar³

et al. 1981

J Clin Microbiol

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Urine specimens to be tested for Ureaplasma urealyticum (T-strain mycoplasma) must often be transported to a central laboratory for identification. To examine survival of these organisms over 2 days of transport, fresh urine samples were tested for the presence of ureaplasmas and then divided into four groups: (i) fresh, (ii) frozen, (iii) preserved with 1% (wt/vol) boric acid at room temperature, and (iv) preserved with 10% dimethyl sulfoxide and frozen. The samples were cultured on both A7 and New York City solid media, and the estimates of survival were compared to that of fresh urine. Less than 10% survival of Ureaplasma was observed in 11 of 14 specimens stored frozen for 2 days without preservation; six specimens lost all organisms. Specimens containing either dimethyl sulfoxide or boric acid showed higher survival rates, although neither method consistently approached the full recovery of the T-strains found with the fresh urine. Ureaplasmas from fresh specimens grew well on both New York City and A7 media; however, the New York City medium proved superior for those preserved with boric acid and for urine samples containing few ureaplasmas. These results indicate that preservation of samples does increase the yield of U. urealyticum from urine samples delayed in transit.

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“…The T-strain mycoplasmas are distinguished from the classical mycoplasmas by their tiny colonies, ability to hydrolyze urea, lower pH optimum for growth, and different susceptibility to several drugs (14). T-strains are common inhabitants of the human genital tract and oral cavity and have also been isolated from other animals (13,15).…”

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confidence: 99%