Nonequilibrium Self-Assembly of a Filament Coupled to ATP/GTP Hydrolysis

Padinhateeri, Ranjith; Lacoste, David; Mallick, Kirone; Joanny, Jean‐François

doi:10.1016/j.bpj.2008.12.3920

Cited by 55 publications

(124 citation statements)

References 45 publications

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“…This has been explained phenomenologically by microtubules being stabilized by a cap of tubulin-GTP, which when lost, triggers de-polymerization catastrophe [30]. Several models have been generated to account for these instabilities [31][32][33][34][35][36][37]. Furthermore, the term "structural plasticity" has been introduced to describe additional changes in filament/polymer structure without change(s) in the chemical state of its bound nucleotide [38].…”

Section: Microtubules and Microtubule-motor Systems In Vitromentioning

confidence: 99%

Cytoskeletal Symmetry Breaking and Chirality: From Reconstituted Systems to Animal Development

Pohl

2015

Symmetry

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Animal development relies on repeated symmetry breaking, e.g., during axial specification, gastrulation, nervous system lateralization, lumen formation, or organ coiling. It is crucial that asymmetry increases during these processes, since this will generate higher morphological and functional specialization. On one hand, cue-dependent symmetry breaking is used during these processes which is the consequence of developmental signaling. On the other hand, cells isolated from developing animals also undergo symmetry breaking in the absence of signaling cues. These spontaneously arising asymmetries are not well understood. However, an ever growing body of evidence suggests that these asymmetries can originate from spontaneous symmetry breaking and self-organization of molecular assemblies into polarized entities on mesoscopic scales. Recent discoveries will be highlighted and it will be discussed how actomyosin and microtubule networks serve as common biomechanical systems with inherent abilities to drive spontaneous symmetry breaking.

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Section: Microtubules and Microtubule-motor Systems In Vitromentioning

confidence: 99%

Cytoskeletal Symmetry Breaking and Chirality: From Reconstituted Systems to Animal Development

Pohl

2015

Symmetry

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“…When the cap is lost or broken by hydrolysis, a catastrophe follows. Numerous models based on this idea have been proposed, which are either discrete (7,8) or continuous (9), with more detailed descriptions also incorporating the mechanical stability of the lattice (10,11).…”

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confidence: 99%

A theory of microtubule catastrophes and their regulation

Brun

Rupp

Ward

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

104

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Dynamic instability, in which abrupt transitions occur between growing and shrinking states, is an intrinsic property of microtubules that is regulated by both mechanics and specialized proteins. We discuss a model of dynamic instability based on the popular idea that growth is maintained by a cap at the tip of the fiber. The loss of this cap is thought to trigger the transition from growth to shrinkage, called a catastrophe. The model includes longitudinal interactions between the terminal tubulins of each protofilament and ''gating rescues'' between neighboring protofilaments. These interactions allow individual protofilaments to transiently shorten during a phase of overall microtubule growth. The model reproduces the reported dependency of the catastrophe rate on tubulin concentration, the time between tubulin dilution and catastrophe, and the induction of microtubule catastrophes by walking depolymerases. The model also reproduces the comet tail distribution that is characteristic of proteins that bind to the tips of growing microtubules.cell biology ͉ cytoskeleton ͉ kinesin ͉ modeling

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“…[6][7][8][9][10][11][14][15][16][17]21,[24][25][26]29,30,32,34 Earlier theoretical models tried to view the dynamics of cytoskeleton filaments in a very phenomenological way. 28 In a more advanced approach, the effects of the structure and interactions between biopolymer subunits on the growth dynamics and force generation in microtubules and actin filaments have been investigated.…”

Section: ■ Introductionmentioning

confidence: 99%

The Role of Multifilament Structures and Lateral Interactions in Dynamics of Cytoskeleton Proteins and Assemblies

Kolomeisky

2015

J. Phys. Chem. B

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Microtubules and actin filaments are biopolymer molecules that are major components of cytoskeleton networks in biological cells. They play important roles in supporting fundamental cellular processes such as cell division, signaling, locomotion, and intracellular transport. In cells, cytoskeleton proteins function under nonequilibrium conditions that are powered by hydrolysis of adenosine triphosphate (ATP) or guanosine triphosphate (GTP) molecules attached to them. Although these biopolymers are critically important for all cellular processes, the mechanisms that govern their complex dynamics and force generation remain not well explained. One of the most difficult fundamental issues is to understand how different components of cytoskeleton proteins interact together. We develop an approximate theoretical approach for analyzing complex processes in cytoskeleton proteins that takes into account the multifilament structure, lateral interactions between parallel protofilaments, and the most relevant biochemical transitions during the biopolymer growth. It allows us to fully evaluate collective dynamic properties of cytoskeleton filaments as well as the effect of external forces on them. It is found that for the case of strong lateral interactions the stall force of the multifilament protein is a linear function of the number of protofilaments. However, for weak lateral interactions, deviations from the linearity are observed. We also show that stall forces, mean velocities, and dispersions are increasing functions of the lateral interactions. Physical−chemical explanations of these phenomena are presented. Our theoretical predictions are supported by extensive Monte Carlo computer simulations.

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Nonequilibrium Self-Assembly of a Filament Coupled to ATP/GTP Hydrolysis

Cited by 55 publications

References 45 publications

Cytoskeletal Symmetry Breaking and Chirality: From Reconstituted Systems to Animal Development

Cytoskeletal Symmetry Breaking and Chirality: From Reconstituted Systems to Animal Development

A theory of microtubule catastrophes and their regulation

The Role of Multifilament Structures and Lateral Interactions in Dynamics of Cytoskeleton Proteins and Assemblies

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