Nonenzyme Cascaded Amplification Biosensor Based on Effective Aggregation Luminescence Caused by Disintegration of Silver Nanoparticles

Abstract

Sensitive and portable quantification of biomarkers has particular significance in the monitoring and treatment of clinical diseases. Conventional immunoassays were accustomed to introducing or incorporating enzymes for signal amplification, which commonly suffered from poor stability and inferior tolerance. Herein, we constructed a novel nonenzyme amplification methodology based on fluorogenic Ag + -tetrazolate aggregation coupled with silver corrosion sensitization for biomarker determination. A significant cascade enhancement strategy was achieved by the valid aggregation luminescence caused by the potent disintegration of silver nanoparticles. Furthermore, efficient magnetic separation was also combined and performed for the rapidity and simplicity of operation. As the target, the detection limit of prostate-specific antigen was 15.66 pg/mL in our designed biosensor. Besides, a good linear relationship was obtained. The designed biosensor demonstrated good specificity and was successfully applied to clinical serum sample detection.

A novel highly sensitive compilation-detachment fluorescence sensing strategy based on RNA-cleavage DNAzyme for MDA-MB-231 breast cancer biomarker determination

“…What makes them even more advantageous is their combination with signaling devices using various biological molecules such as DNA, aptamers, and antibodies. [46][47][48][49] As mentioned earlier, most of the reported RNA-cleaving DNA enzymes have been used to detect toxic metal ions. However, in recent years, several RNA-cleaving DNA enzymes that can be used to detect some biological molecules, such as bacteria, have been selected by an in vitro selection procedure.…”

“…What makes them even more advantageous is their combination with signaling devices using various biological molecules such as DNA, aptamers, and antibodies. 46–49…”

High-Throughput Approach for Facile Access to Hetero-Dinuclear Synergistic Metal Complex for H2O2 Activation and Its Implications

“…H 2 O 2 , one of the most important, efficient, and environmentally friendly oxidants, is widely used in chemical synthesis, − bleaching, wastewater treatment, , and biosensing. , However, despite its high formal oxidation potential, it reacts very slowly with some substrates. To effectively utilize its oxidizing power, activation is required to improve its reactivity toward a substrate through the formation of reactive intermediates, such as metal-(hydro)­peroxo or high-valent metal-oxo species, or the generation of reactive oxygen species (ROS) whose reactivity with substrates is remarkably higher than that of H 2 O 2 itself. , An effective strategy for H 2 O 2 activation is using metal complexes that are designed to mimic the characteristics of active sites of the H 2 O 2 -related enzymes, such as peroxidase, catalyzing the oxidation of various compounds in the presence of H 2 O 2 .…”

Programmable molecular circuit discriminates multidrug-resistant bacteria

“…Molecular recognition elements that can anchor the bacterial surface protein with high specificity and affinity in a short time provide an innovative tool for signal conversion and break the bottleneck of lysis steps [ [19] , [20] , [21] ]. Among those molecular recognition elements ranging from antibodies to cellular wall-binding domain proteins, aptamers with easy reprogramming potential and thermal stability have attracted increasing attention [ [22] , [23] , [24] , [25] ].…”