Nondestructive multiplex detection of foodborne pathogens with background microflora and symbiosis using a paper chromogenic array and advanced neural network

Jia, Zhen; Luo, Yaguang; Wang, Dayang; Dinh, Quynh N.; Lin, Sophia; Sharma, Arnav; Block, Ethan M.; Yang, Manyun; Gu, Tingting; Pearlstein, Arne J.; Yu, Hengyong; Zhang, Boce

doi:10.1016/j.bios.2021.113209

Cited by 28 publications

(11 citation statements)

References 71 publications

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“…In recent years, with the rapid development in the field of materials, the definition between traditional paper and flexible films has become blurred, and some flexible films with flexible or porous structures have been defined as paper [ 96 ]. Some treatment and processing of the microstructure of the paper is usually necessary to create paper-based microfluidic devices, including wax printing, printing, photolithography, etching, chemical alteration of the fiber surface, and other methods [ 97 , 98 , 99 , 100 ].…”

Section: Different Materials and Preparation Methods For Microfluidic...mentioning

confidence: 99%

Porous Structural Microfluidic Device for Biomedical Diagnosis: A Review

et al. 2023

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Microfluidics has recently received more and more attention in applications such as biomedical, chemical and medicine. With the development of microelectronics technology as well as material science in recent years, microfluidic devices have made great progress. Porous structures as a discontinuous medium in which the special flow phenomena of fluids lead to their potential and special applications in microfluidics offer a unique way to develop completely new microfluidic chips. In this article, we firstly introduce the fabrication methods for porous structures of different materials. Then, the physical effects of microfluid flow in porous media and their related physical models are discussed. Finally, the state-of-the-art porous microfluidic chips and their applications in biomedicine are summarized, and we present the current problems and future directions in this field.

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Section: Different Materials and Preparation Methods For Microfluidic...mentioning

confidence: 99%

Porous Structural Microfluidic Device for Biomedical Diagnosis: A Review

et al. 2023

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“…This assay is cheap (<US$0.1), does not require enrichment, culturing, incubation or sample-preparation steps, and is thus highly attractive for POC applications. In addition, it also performs against background microflora 65 . This is important because VOCs can also be emitted by food products and non-pathogenic microorganisms.…”

Section: Paper-based Sensing Platformsmentioning

confidence: 99%

Paper-based sensors for bacteria detection

et al. 2023

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The detection of pathogenic bacteria is essential to prevent and treat infections and to provide food security. Current gold-standard detection techniques, such as culture-based assays and polymerase chain reaction, are time-consuming and require centralized laboratories. Therefore, efforts have focused on developing point-of-care devices that are fast, cheap, portable and do not require specialized training. Paper-based analytical devices meet these criteria and are particularly suitable to deployment in low-resource settings. In this Review, we highlight paper-based analytical devices with substantial point-of-care applicability for bacteria detection and discuss challenges and opportunities for future development.

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“…38 Colorimetric assays have been successfully developed for single species of interest, 39,40 as well as for identification of a range of bacterial pathogens. 41,42 Paper plates are portable, inexpensive, biodegradable, and do not require handling by trained personnel, yet are compatible with microplate readers allowing the measurement of absorbance as well as fluorescence. 32 The morphology of the paper presents a network of microfibers that provide efficient adsorption of the analyte with low loading volume.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Paper-based analytical devices (micro-PADS) and paper-based enzyme-linked immunosorbent assay (ELISA) are examples of the recently developed paper-based platforms as diagnostic tools. − Lab-on-paper has been employed for detection of blood type, food quality control, biomarkers for cancer, and environmental monitoring. − Paper-based sensing is now being used for the identification of foodborne and waterborne pathogens. , Much effort is directed toward the development of point-of-care devices using various paper-based biosensors, including those for bacterial diagnostics . Colorimetric assays have been successfully developed for single species of interest, , as well as for identification of a range of bacterial pathogens. , …”

Section: Introductionmentioning

confidence: 99%

Simpler Procedure and Improved Performance for Pathogenic Bacteria Analysis with a Paper-Based Ratiometric Fluorescent Sensor Array

et al. 2022

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Bacterial infections are the leading cause of morbidity and mortality in the world, particularly due to a delay in treatment and misidentification of the bacterial species causing the infection. Therefore, rapid and accurate identification of these pathogens has been of prime importance. The conventional diagnostic techniques include microbiological, biochemical, and genetic analyses, which are time-consuming, require large sample volumes, expensive equipment, reagents, and trained personnel. In response, we have now developed a paper-based ratiometric fluorescent sensor array. Environment-sensitive fluorescent dyes (3-hydroxyflavone derivatives) pre-adsorbed on paper microzone plates fabricated using photolithography, upon interaction with bacterial cell envelopes, generate unique fluorescence response patterns. The stability and reproducibility of the sensor array response were thoroughly investigated, and the analysis procedure was refined for optimal performance. Using neural networks for response pattern analysis, the sensor was able to identify 16 bacterial species and recognize their Gram status with an accuracy rate greater than 90%. The paper-based sensor was stable for up to 6 months after fabrication and required 30 times lower dye and sample volumes as compared to the analogous solution-based sensor. Therefore, this approach opens avenues to a state-of-the-art diagnostic tool that can be potentially translated into clinical applications in low-resource environments.

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Nondestructive multiplex detection of foodborne pathogens with background microflora and symbiosis using a paper chromogenic array and advanced neural network

Cited by 28 publications

References 71 publications

Porous Structural Microfluidic Device for Biomedical Diagnosis: A Review

Porous Structural Microfluidic Device for Biomedical Diagnosis: A Review

Paper-based sensors for bacteria detection

Simpler Procedure and Improved Performance for Pathogenic Bacteria Analysis with a Paper-Based Ratiometric Fluorescent Sensor Array

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