Nondestructive mechanical characterization of developing biological tissues using inflation testing

Oomen, Pim J A; Kelle, Mathieu A.J. van; Oomens, C.W.J.; Bouten, Cvc Carlijn; Loerakker, Sandra

doi:10.1016/j.jmbbm.2017.07.009

Cited by 7 publications

(10 citation statements)

References 42 publications

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“…with k 1 and k 2 material parameters, (l i ) 2 ¼ C:(e i f e i f ) the squared elastic fibre stretch, and k8l the Macaulay brackets to enforce that the fibres only resist tension. The ability of this constitutive model to adequately describe the mechanical behaviour of TE constructs was previously demonstrated [37].…”

Section: Mechanical Properties and Mechanical State During Culture 251 Constitutive Modelmentioning

confidence: 90%

“…The sample profile as a function of pressure was tracked in the B-mode images (frame rate 25 Hz, resolution 21 px mm 21 ) using a custom MATLAB script [37]. First, the two attachment points of the constructs were selected after which for each frame the tissue apex was manually selected.…”

Section: Non-destructive Geometrical and Mechanical Characterization mentioning

confidence: 99%

“…From each nondestructive mechanical test, the construct's profile as a function of pressure was recorded at each time point. Using this information, the material properties j (here j ¼ [m, k 1 , k 2 ]) of the constructs at each time point were determined using a twostep inverse method that was previously developed [37]. In the first step, shell theory was used to obtain a rapid analytical estimate of the material properties.…”

Section: Estimation Of Materials Propertiesmentioning

confidence: 99%

“…To this end, a recently developed in vitro bioreactor [36] was used to culture two groups of TE cardiovascular constructs with different initial thicknesses (relevant for cardiovascular tissue engineering), under the same dynamic loading conditions. In order to investigate whether geometrical and/or mechanical equilibrium was established in the TE constructs, temporal changes in tissue geometry and mechanical state were quantified at several time points during development, by means of non-destructive ultrasound imaging and inverse analysis [37]. At the end of culture, the TE constructs' composition and structure were determined by biochemical assays, histology and in-plane collagen organization.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Initial scaffold thickness affects the emergence of a geometrical and mechanical equilibrium in engineered cardiovascular tissues

Kelle

Oomen

Broek

et al. 2018

J. R. Soc. Interface.

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In situ cardiovascular tissue-engineering can potentially address the shortcomings of the current replacement therapies, in particular, their inability to grow and remodel. In native tissues, it is widely accepted that physiological growth and remodelling occur to maintain a homeostatic mechanical state to conserve its function, regardless of changes in the mechanical environment. A similar homeostatic state should be reached for tissue-engineered (TE) prostheses to ensure proper functioning. For in situ tissue-engineering approaches obtaining such a state greatly relies on the initial scaffold design parameters. In this study, it is investigated if the simple scaffold design parameter initial thickness, influences the emergence of a mechanical and geometrical equilibrium state in in vitro TE constructs, which resemble thin cardiovascular tissues such as heart valves and arteries. Towards this end, two sample groups with different initial thicknesses of myofibroblast-seeded polycaprolactone-bisurea constructs were cultured for three weeks under dynamic loading conditions, while tracking geometrical and mechanical changes temporally using non-destructive ultrasound imaging. A mechanical equilibrium was reached in both groups, although at different magnitudes of the investigated mechanical quantities. Interestingly, a geometrically stable state was only established in the thicker constructs, while the thinner constructs’ length continuously increased. This demonstrates that reaching geometrical and mechanical stability in TE constructs is highly dependent on functional scaffold design.

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Section: Mechanical Properties and Mechanical State During Culture 251 Constitutive Modelmentioning

confidence: 90%

Section: Non-destructive Geometrical and Mechanical Characterization mentioning

confidence: 99%

Section: Estimation Of Materials Propertiesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Initial scaffold thickness affects the emergence of a geometrical and mechanical equilibrium in engineered cardiovascular tissues

Kelle

Oomen

Broek

et al. 2018

J. R. Soc. Interface.

Self Cite

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“…Subsequently, the membrane is inflated and deflated, which generates a bulging mechanism that stimulates the cultured tissue multiaxially. The mechanical properties of the free-standing membrane were characterized using the bulge test equation (Oomen et al, 2017;van Kelle et al, 2017).…”

Section: Multiaxial Stimulationmentioning

confidence: 99%

Reflecting on Mechanical Functionalities in Bioreactors for Tissue Engineering Purposes

Nadhif

Assyarify

Waafi

et al. 2020

IJTech

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Many articles have reported a correlation between the use of mechanical stimulation and an enhancement in cultivation of various tissues engineered in bioreactors. The enhancement includes improvements in cell growth, proliferation, and functionalities. The aim of this report is to review the mechanical functionalities of tissue engineering bioreactors in terms of the forms of stimulation, types of stress, actuators, supporting modules, and, most importantly, efficacy. The Google Scholar database was searched for relevant articles. Three forms of simulation were reported: uniaxial, biaxial, and multiaxial. The types of stress exerted by bioreactors include compression, tension, shear, and dynamic stresses, which are applied solely or mutually depending on the number of axes involved. Mechanical stimulation could be actuated by stepper motors, pistons, pneumatic pumps, diaphragm pumps, piezoelectric systems, or dielectric charges. Additional modules, such as incubators, flow perfusion systems, ultrasound sensors, movement controls, and electrodes, can also support the mechanical functions of bioreactors. The efficacy of a bioreactor could be determined by investigating the biomechanical and histological properties of the engineered tissues. To facilitate the development of mechanical functionalities for tissue engineering bioreactors in the future, a seven-step framework is proposed.

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Numerical investigation on circular and elliptical bulge tests for inverse soft tissue characterization

Gasparotti

Vignali

Quartieri

et al. 2023

Biomech Model Mechanobiol

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Nondestructive mechanical characterization of developing biological tissues using inflation testing

Cited by 7 publications

References 42 publications

Initial scaffold thickness affects the emergence of a geometrical and mechanical equilibrium in engineered cardiovascular tissues

Initial scaffold thickness affects the emergence of a geometrical and mechanical equilibrium in engineered cardiovascular tissues

Reflecting on Mechanical Functionalities in Bioreactors for Tissue Engineering Purposes

Numerical investigation on circular and elliptical bulge tests for inverse soft tissue characterization

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