Nondestructive evaluation of osteogenic differentiation in tissue‐engineered constructs

Hong, Liu; Peptan, Ioana A.; Xu, Huihui; Magin, Richard L.

doi:10.1002/jor.20140

Cited by 7 publications

(5 citation statements)

References 40 publications

(43 reference statements)

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“…At day 7, cells were collected and analyzed for osteogenic differentiation markers and steroid-associated growth factors and receptors using quantitative real-time PCR. To investigate the osteogenic potentials of MSCs seeded in 3-dimensional scaffolds, we created tissue-engineered constructs by seeding passage-1 MSCs on gelatin sponges as described in our previous studies [18]. The sterile gelatin sponge (Gelfoam @ , Pharmacia & Upjohn, Kalamazoo, MI) was trimmed into a cube (4×4×4 mm 3 ) and pre-wetted with DMEM medium.…”

Section: Methodsmentioning

confidence: 99%

Steroid regulation of proliferation and osteogenic differentiation of bone marrow stromal cells: A gender difference

Hong

Sultana

Paulius

et al. 2009

The Journal of Steroid Biochemistry and Molecular Biology

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Bone marrow mesenchymal stem cells (MSCs) are considered a potential cell source for stem cell-based bone tissue engineering. However, noticeable limitations of insufficient supply and reduction of differentiation potential impact the feasibility of their clinical application. This study investigated the in vitro function of steroids and gender differences on the proliferation and differentiation of rat MSCs. Bone marrow MSCs of age-matched rats were exposed to proliferation and osteogenic differentiation media supplements with various concentrations of 17β-estradiol (E2) and dexamethasone. Cell proliferation was measured by MTS assay; osteogenic markers and steroid-associated growth factors and receptors were evaluated by ELISA and real-time PCR. The results revealed that supplements of E2 and dexamethasone increase MSC proliferation in a biphasic manner. The optimal dose and interaction of steroids required to improve MSC proliferation effectively varied depending on the gender of donors. Supplementation of E2 effectively improves osteogenic differentiation markers including ALP, osteocalcin and calcium levels for MSCs isolated from both male and female donors. The mRNA of TGF-β1 and BMP-7 are also up-regulated. However, effective doses to maximally improve osteogenic potentials and growth factors for MSCs are different between male and female donors. The relationship between steroid receptors, osteogenic markers and cytokines are also varied by genders. The outcomes of the present study strongly indicate that steroids potentially function as an effective modulator to improve the capacity of MSCs in bone regeneration. It provides crucial information for improving and optimizing MSCs for future clinical application of bone regeneration.

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Section: Methodsmentioning

confidence: 99%

Steroid regulation of proliferation and osteogenic differentiation of bone marrow stromal cells: A gender difference

Hong

Sultana

Paulius

et al. 2009

The Journal of Steroid Biochemistry and Molecular Biology

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“…233 MRI has shown to be a promising imaging modality for the assessment of musculoskeletal tissue-engineered constructs. 7,[234][235][236][237][238][239] In a study of Washburn et al, for instance, an inverse relationship between MR relaxation times and mineral concentration was found after culturing osteoblasts on poly-(ethyl methacrylate) scaffolds. 240 Chesnick et al…”

Section: Magnetic Resonance Imagingmentioning

confidence: 99%

Methods of Monitoring Cell Fate and Tissue Growth in Three-Dimensional Scaffold-Based Strategies forIn VitroTissue Engineering

Leferink

Blitterswijk

Moroni

2016

Tissue Engineering Part B: Reviews

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In the field of tissue engineering, there is a need for methods that allow assessing the performance of tissue-engineered constructs noninvasively in vitro and in vivo. To date, histological analysis is the golden standard to retrieve information on tissue growth, cellular distribution, and cell fate on tissue-engineered constructs after in vitro cell culture or on explanted specimens after in vivo applications. Yet, many advances have been made to optimize imaging techniques for monitoring tissue-engineered constructs with a sub-mm or μm resolution. Many imaging modalities have first been developed for clinical applications, in which a high penetration depth has been often more important than lateral resolution. In this study, we have reviewed the current state of the art in several imaging approaches that have shown to be promising in monitoring cell fate and tissue growth upon in vitro culture. Depending on the aimed tissue type and scaffold properties, some imaging methods are more applicable than others. Optical methods are mostly suited for transparent materials such as hydrogels, whereas magnetic resonance-based methods are mostly applied to obtain contrast between hard and soft tissues regardless of their transparency. Overall, this review shows that the field of imaging in scaffold-based tissue engineering is developing at a fast pace and has the potential to overcome the limitations of destructive endpoint analysis.

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“…For the purpose of industrialization, higher expectations for standardization are now required for automation technology; platforms need to improve both quality of results and cost effectiveness (Ratcliffe ; Smith ). Recently, advances in novel imaging technologies, used to support image‐based cell evaluation, have improved performance to the point where quantitative evaluation of detailed cellular events is now possible (Becker and Madany, ; Erdmann et al, ; Hong et al, ; Kino‐oka et al, ; Li et al, ; Platt et al, ; Poirier‐Quinot et al, ; Seiler et al, ; Unadkat et al, ). Non‐destructive imaging methods have shown particular compatibility with cell therapy, which requires intact cells for therapy during and after their evaluations.…”

Section: Introductionmentioning

confidence: 99%

Characterization of time‐course morphological features for efficient prediction of osteogenic potential in human mesenchymal stem cells

Matsuoka

Takeuchi

Agata

et al. 2014

Biotech & Bioengineering

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Human bone marrow mesenchymal stem cells (hBMSCs) represents one of the most frequently applied cell sources for clinical bone regeneration. To achieve the greatest therapeutic effect, it is crucial to evaluate the osteogenic differentiation potential of the stem cells during their culture before the implantation. However, the practical evaluation of stem cell osteogenicity has been limited to invasive biological marker analysis that only enables assaying a single end-point. To innovate around invasive quality assessments in clinical cell therapy, we previously explored and demonstrated the positive predictive value of using time-course images taken during differentiation culture for hBMSC bone differentiation potential. This initial method establishes proof of concept for a morphology-based cell evaluation approach, but reveals a practical limitation when considering the need to handle large amounts of image data. In this report, we aimed to scale-down our proposed method into a more practical, efficient modeling scheme that can be more broadly implemented by physicians on the frontiers of clinical cell therapy. We investigated which morphological features are critical during the osteogenic differentiation period to assure the performance of prediction models with reduced burden on image acquisition. To our knowledge, this is the first detailed characterization that describes both the critical observation period and the critical number of time-points needed for morphological features to adequately model osteogenic potential. Our results revealed three important observations: (i) the morphological features from the first 3 days of differentiation are sufficiently informative to predict bone differentiation potential, both activities of alkaline phosphatase and calcium deposition, after 3 weeks of continuous culture; (ii) intervals of 48 h are sufficient for measuring critical morphological features; and (iii) morphological features are most accurately predictive when early morphological features from the first 3 days of differentiation are combined with later features (after 10 days of differentiation). Biotechnol. Bioeng. 2014;111: 1430–1439.

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Nondestructive evaluation of osteogenic differentiation in tissue‐engineered constructs

Cited by 7 publications

References 40 publications

Steroid regulation of proliferation and osteogenic differentiation of bone marrow stromal cells: A gender difference

Steroid regulation of proliferation and osteogenic differentiation of bone marrow stromal cells: A gender difference

Methods of Monitoring Cell Fate and Tissue Growth in Three-Dimensional Scaffold-Based Strategies forIn VitroTissue Engineering

Characterization of time‐course morphological features for efficient prediction of osteogenic potential in human mesenchymal stem cells

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