Noncytotoxic Mn-doped ZnSe/ZnS quantum dots for biomedical applications

Plumley, John B.; Akins, Brian A.; Alas, Gema J.; Fetrow, Madalyn E.; Nguyen, Jane; Jain, Priyanka; Yang, S.Q.; Brandt, Yekaterina I.; Smolyakov, Gennady A.; Ornatowski, Wojciech; Milligan, Erin D.; Osiński, Marek

doi:10.1117/12.2043293

Cited by 3 publications

(5 citation statements)

References 28 publications

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“…To compare the biological activity of L‐Cys and D‐Cys ZnS:Mn QDs, we studied their effect on A549 cell viability. It was anticipated that the cytotoxicity of the ZnS:Mn QDs would be significantly lower compared to that of cadmium‐based QDs . Nonetheless, ZnS‐based QDs still exhibited cytotoxicity, which was dose‐dependent.…”

Section: Resultsmentioning

confidence: 99%

“…It was anticipated that the cytotoxicity of the ZnS:Mn QDs would be significantly lower compared to that of cadmiumbased QDs. [33][34][35][37][38][39] Nonetheless, ZnS-based QDs still exhibited cytotoxicity, which was dose-dependent. A wide range of NPs, even consisting of nontoxic elements, revealed nanocytotoxicity.…”

Section: Cell Viability Assaymentioning

confidence: 99%

“…Heavy‐metal‐free QDs, based on a ZnS core doped with manganese ions (ZnS:Mn), are a unique and relatively new type of colloidal nanomaterials that possess a number of advantages in comparison with traditional Cd‐based QDs . First, ZnS:Mn QDs do not display heavy‐metal‐associated toxicity within living cells and organisms . Second, the PL maximum of ZnS:Mn is located at 585 nm, at the spectral range in which tissue autofluorescence is negligible, meaning the QDs are ideal labels for bioimaging .…”

Section: Introductionmentioning

confidence: 99%

“…36 First, ZnS:Mn QDs do not display heavy-metal-associated toxicity within living cells and organisms. 35,[37][38][39] Second, the PL maximum of ZnS:Mn is located at 585 nm, at the spectral range in which tissue autofluorescence is negligible, meaning the QDs are ideal labels for bioimaging. 39,40 In addition, ZnS:Mn QDs can be excited by three-photon excitation using a 920 nm light source within the near-infrared window of biological tissue.…”

Section: Introductionmentioning

confidence: 99%

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Enantioselective cytotoxicity of ZnS:Mn quantum dots in A549 cells

et al. 2017

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Chirality strongly influences many biological properties of materials, such as cell accumulation, enzymatic activity, and toxicity. In the past decade, it has been shown that quantum dots (QDs), fluorescent semiconductor nanoparticles with unique optical properties, can demonstrate optical activity due to chiral ligands bound on their surface. Optically active QDs could find potential applications in biomedical research, therapy, and diagnostics. Consequently, it is very important to investigate the interaction of QDs capped with chiral ligands with living cells. The aim of our study was to investigate the influence of the induced chirality of Mn-doped ZnS QDs on the viability of A549 cells. These QDs were stabilized with D- and L-cysteine using a ligand exchange technique. The optical properties of QDs were studied using UV-Vis, photoluminescence (PL), and circular dichroism (CD) spectroscopy. The cytotoxicity of QDs was investigated by high content screening analysis. It was found that QDs stabilized by opposite ligand enantiomers, had identical PL and UV-Vis spectra and mirror-imaged CD spectra, but displayed different cytotoxicity: QDs capped with D-cysteine had greater cytotoxicity than L-cysteine capped QDs.

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Section: Resultsmentioning

confidence: 99%

Section: Cell Viability Assaymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Enantioselective cytotoxicity of ZnS:Mn quantum dots in A549 cells

et al. 2017

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“…Toxicity resulting from composition can be addressed through the use of cadmiumfree QDs [20,21]. Several alternative compositions are now available, such as InP- [22,23] and Mn-doped ZnSe-or ZnS-based compositions [24][25][26][27][28][29][30][31]. Mn-doped QDs are particularly interesting because they emit photoluminescence from their doped centres rather than delocalised surface states, increasing photostability against surface chemical-or photooxidation [32].…”

Section: Introductionmentioning

confidence: 99%

Effect of Micelle Encapsulation on Toxicity of CdSe/ZnS and Mn-Doped ZnSe Quantum Dots

et al. 2021

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The optical properties of quantum dots (QD) make them excellent candidates for bioimaging, biosensing, and therapeutic applications. However, conventional QDs are comprised of heavy metals (e.g., cadmium) that pose toxicity challenges in biological systems. Synthesising QDs without heavy metals or introducing thick surface coatings, e.g., by encapsulation in micelles, can reduce toxicity. Here, we examined the toxicity of micelle encapsulated tetrapod-shaped Mn-doped ZnSe QDs, comparing them to 3-mercaptopropionic acid (MPA)-capped Mn-doped ZnSe QDs prepared by ligand exchange and commercial CdSe/ZnS QD systems that were either capped with MPA or encapsulated in micelles. HepG2 cell treatment with MPA-coated CdSe/ZnS QDs resulted in a dose-dependent reduction of viability (MTT assay, treatment at 0–25 μg/mL). Surprisingly, no reactive oxygen species (ROS) or apoptotic signaling was observed, despite evidence of apoptotic behavior in flow cytometry. CdSe/ZnS QD micelles showed minimal toxicity at doses up to 25 μg/mL, suggesting that thicker protective polymer layers reduce cytotoxicity. Despite their shape, neither MPA- nor micelle-coated Mn-doped ZnSe QDs displayed a statistically significant toxicity response over the doses investigated, suggesting these materials as good candidates for bioimaging applications.

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Theoretical Investigation on Structural and Magnetic Properties of Mn-doped and C co-doped Zn12Se12 Nanoclusters

Chen

Fang

Liu

2021

CNANO

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Background: Mn doped ZnSe low dimensional materials are attractive for different biological labels, gene silencing and dilute-magnetic device. ZnSe clusters are one of the basic building blocks of quantum dots and even clusterassembled nanodevices, Stable structures of undoped ZnSe clusters were established by previous pioneering work, and the Mn doped ZnSe clusters had been investigated, but the stable cluster in ferromagnetic state haven't been found yet. Objective: Our work mainly concentrates on Mn doped clusters (Mn2Zn10Se12) and C codoped clusters (Mn2Zn10CSe11) structure, magnetic properties through theoretical calculations. Methods: First principle density functional theory calculation with Dmol3 are used to execute all calculations. Results: Mn atoms prefer to substitute nearest neighbor Zn atom sites in rhombi part, and C atom prefers to occupy Se atom sites with shortest Mn-C bond length in Zn12Se12 nanocluster doping. Mn doped clusters (Mn2Zn10Se12) were in antiferromagnetic states and the most stable C codoped clusters (Mn2Zn10CSe11) were in ferromagnetic states. Magnetic behavior localized at the 3d orbitals of transitional metal Mn, 4p orbital of atom Se and 2p orbital of C atom. Mn2Zn10Se12 clusters were in antiferromagnetic states as the p-d hybridization introduced Mn-Mn superexchange mechanism. For the ferromagnetism of Mn2Zn10Se12 nanocluster, hole mediated double exchange mechanism introduced by C atom p-d hole state hybridization has been suggested. Conclusion: The codoping of C atom can stabilize the ferromagnetism of clusters through hole mediated double exchange mechanism, which may be meaningful for the exploring materials for cluster-assembled spin-electronic devices.

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Noncytotoxic Mn-doped ZnSe/ZnS quantum dots for biomedical applications

Cited by 3 publications

References 28 publications

Enantioselective cytotoxicity of ZnS:Mn quantum dots in A549 cells

Enantioselective cytotoxicity of ZnS:Mn quantum dots in A549 cells

Effect of Micelle Encapsulation on Toxicity of CdSe/ZnS and Mn-Doped ZnSe Quantum Dots

Theoretical Investigation on Structural and Magnetic Properties of Mn-doped and C co-doped Zn12Se12 Nanoclusters

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