Noncytopathic bovine viral diarrhea virus in a system for in vitro production of bovine embryos

Zurovac, O.; Stringfellow, D.A.; Brock, Kenny V.; Riddell, M.G.; Wright, James C.

doi:10.1016/0093-691x(94)90500-i

Cited by 31 publications

(4 citation statements)

References 11 publications

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“…There is also the potential for contaminated reproductive fluids and tissues to be collected at an abattoir for use in in vitro embryo production (Bielanski et al 1993). Our study showed, as has been shown previously (Zurovac et al 1994;Stringfellow et al 1997), that the presence of UTC from PI animals in in vitro production will contaminate fertilization and culture drops leading to contamination of embryos. However, the purpose of this study was to determine whether or not embryos derived from in vitro fertilization of oocytes from PI cattle would contain infectious virus.…”

Section: Discussionsupporting

confidence: 81%

“…These studies suggested that oocytes from BVDV-infected animals can be used for in vitro embryo production, and resultant embryos be free of virus. However, these conclusions were not supported when BVDV was artificially introduced into an in vitro production system (Bielanski and Jordan 1996;Stringfellow et al 2000) or when BVDV infected uterine tubal cells (UTC) were used for co-culture (Zurovac et al 1994;Stringfellow et al 1997).…”

Section: Introductionmentioning

confidence: 99%

“…However, these conclusions were not supported when BVDV was artificially introduced into an in vitro production system (Bielanski and Jordan 1996; Stringfellow et al. 2000) or when BVDV infected uterine tubal cells (UTC) were used for co‐culture (Zurovac et al. 1994; Stringfellow et al.…”

Section: Introductionmentioning

confidence: 99%

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Amplification of Bovine Viral Diarrhoea Virus Introduced into an In Vitro Embryo Production System Via Oocytes from Persistently Infected Cattle

Marley

Givens

Galik

et al. 2009

Reprod Domestic Animals

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The purpose of this study was to determine whether or not embryos derived from in vitro fertilization of oocytes from persistently infected (PI) cattle would contain infectious virus.Three in vitro embryo production treatment groups were assessed: 1) oocytes and uterine tubal cells (UTC) free of bovine viral diarrhoea virus (BVDV) (negative control), 2)oocytes free of BVDV fertilized and cultured in media containing UTC obtained from PI heifers, and 3) oocytes from PI heifers fertilized and cultured in media containing UTC free of BVDV. The developmental media, UTC and embryos (individual or groups of five) were assayed for virus.Virus was not isolated from any samples in treatment group 1.As shown in previous studies, a proportion of embryo samples were positive for BVDV in treatment group 2. In treatment group 3, the virus associated with the oocytes contaminated the developmental media and infected susceptible co-culture cells used during fertilization and culture. In addition, 65% (11/17) of the degenerated ova from treatment group 3 had infectious virus associated with them. While none of the ova developed into transferable embryos, the study did confirm that use of oocytes from PI cows could lead to amplification of BVDV and cross contamination during in vitro embryo production.

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Section: Discussionsupporting

confidence: 81%

Section: Introductionmentioning

confidence: 99%

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Amplification of Bovine Viral Diarrhoea Virus Introduced into an In Vitro Embryo Production System Via Oocytes from Persistently Infected Cattle

Marley

Givens

Galik

et al. 2009

Reprod Domestic Animals

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“…Moreover, conflicting results were obtained with oocytes originating from infected donors. Some studies confirmed the insufficient effects of washing on IVF embryos infected with BHV-1 [9], BVDV [66], whereas no evidence of pathogen after washing was observed with BVDV [8]. The discrepancies between these results raise doubts on the transposition of in vitro contamination hardjo in the pores, matrix and channels of ZP and in the embryonic cells, indicating the ability of these pathogenic agents to attach the ZP or penetrate into the embryos.…”

Section: Risks Of Disease Transmission Via Contaminated Ivf Embryosmentioning

confidence: 70%

Risks of transmissible diseases in relation to embryo transfer

et al. 2001

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Effect of bovine herpesvirus-1 or bovine viral diarrhea virus on development of in vitro-produced bovine embryos

et al. 1999

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Noncytopathic bovine viral diarrhea virus in a system for in vitro production of bovine embryos

Cited by 31 publications

References 11 publications

Amplification of Bovine Viral Diarrhoea Virus Introduced into an In Vitro Embryo Production System Via Oocytes from Persistently Infected Cattle

Amplification of Bovine Viral Diarrhoea Virus Introduced into an In Vitro Embryo Production System Via Oocytes from Persistently Infected Cattle

Risks of transmissible diseases in relation to embryo transfer

Effect of bovine herpesvirus-1 or bovine viral diarrhea virus on development of in vitro-produced bovine embryos

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