Noncultured epidermal suspension transplantation for the treatment of stable vitiligo in children and adolescents

Sahni, Kanika; Parsad, Davinder; Kanwar, Amrinder J.

doi:10.1111/j.1365-2230.2011.04065.x

Cited by 26 publications

(43 citation statements)

References 19 publications

(37 reference statements)

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“…[16] In another study, 79% of vitiligo patients gained >90% repigmentation at the end of 1 year post transplantation. [17] On the other hand some groups reported fair results. Back et al .…”

Section: Discussionmentioning

confidence: 99%

“…[1218] Different studies have used different volume of cell suspension in addition to different media to resuspend the cells. For example, a group used phosphate buffer saline[17] while another used melanocyte media for making cell suspension. [18] The other difference is the route of transplantation.…”

Section: Discussionmentioning

confidence: 99%

“…[11] Since that time, a couple of articles have been published using non cultured keratinocyte melanocyte suspension for treatment of skin depigmentation in diseases such as vitiligo and post burn leukoderma. [34812–19] Some articles are in favour of good results with this method[141719] ; some of them claim that good results are just observed in a portion of patients,[81213] while others have disappointing outcomes. [3]…”

Section: Introductionmentioning

confidence: 99%

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Comparison of intradermal injection of autologous epidermal cell suspension vs. spraying of these cells on dermabraded surface of skin of patients with post-burn hypopigmentation

et al. 2013

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Introduction:One of the most important complications after burning is hypo/depigmentation. This study was designed to compare two methods of cell spray and intradermal injection of epidermal cell suspension for treatment of burn induced hypopigmentation.Material and Methods:In this study, 28 patients with post burn hypo/depigmentation were selected and divided in 2 groups. A small skin biopsy was taken from normal skin of patients in operation room and epidermal cell suspension was prepared using NaBr 4N and trypsin. In the first group, the epidermal cell suspension was sprayed on the wound surface and then the area was dressed with amniotic membrane and gauze. In the second group, the cell suspension was injected in intradermal manner in the hypopigmented area. The patients were followed up and to evaluate the effect of the cells, photos were taken from the area before operation and also at follow-up. Clinical evaluation was done by the surgeon and a clinical score between “0” to “4” was used to demonstrate the clinical status from poor to excellent pigmentation. Skin biopsies were taken from depigmented area before and after interventions. Melanocytes were stained using anti S100 antibody and were counted in ×400 magnification fields.Results:Eighteen patients were in cell spray and 10 were in cell injection groups. Mean change of pigmentation in two group showed that there was no statistical significant differences in pigmentation between two groups, (P value = 0.52) although a limited improvement in pigmentation status was observed in both groups. Regarding melanocyte numbers per field, there was not a significant difference between two groups and also before and after interventions, but melanocyte number increased after treatment in both groups.Conclusion:We did not find noticeable differences between cell spray and intradermal injection methods. Although both methods showed a limited effect on pigmentation of depigmented skin, the clinical results were not satisfactorily for both patients and clinicians.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Comparison of intradermal injection of autologous epidermal cell suspension vs. spraying of these cells on dermabraded surface of skin of patients with post-burn hypopigmentation

et al. 2013

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“…Cellular grafting techniques have advantages over tissue grafts such as punch grafts or suction blister epidermal grafts, including greater uniformity of pigmentation, lower risk of skin-texture abnormalities such as cobblestoning, and the ability to cover larger areas of vitiligo with smaller graft sizes. Noncultured epidermal suspension transplantation in stable vitiligo was first described by Gauthier and Surleve-Bazeille [25], and a recent paper reports the use of this technique in children and adolescents [26]. However, the donor skin size to recipient skin size ratio is between 1 : 3 and 1 : 10.…”

Section: Discussionmentioning

confidence: 99%

Amniotic Membrane as a Scaffold for Melanocyte Transplantation in Patients with Stable Vitiligo

Redondo

Azcárate

Marques

et al. 2011

Dermatology Research and Practice

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Vitiligo is an acquired skin disease that significantly impacts the quality of life of patients. Medical treatment of vitiligo includes the use of melanocyte transplant, but the results are variable. We have treated 4 patients with either focal or generalized stable vitiligo using a graft of autologous melanocytes' culture on a denuded amniotic membrane (AM). A culture biopsy was obtained in every patient and grown in melanocytes' media for 10-14 days after which cells were transferred to a denuded AM and transplanted into the achromic lesions. Patients were followed for up to 6 months using clinical assessment of achromic lesions. Treated areas ranged between 4 cm 2 and 210.6 cm 2 . Response to treatment was excellent in all patients with 90-95% repigmentation success rate. Our results demonstrate that transplantation of autologous melanocytes cultured on AM is a new, simple, and effective treatment for stable vitiligo.

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“…Sahni et al . [48] used the similar technique and observed 75-90% repigmentation after one year of procedure.…”

Section: Therapiesmentioning

confidence: 99%

Childhood vitiligo: Treatment paradigms

Kanwar

Kumaran

2012

Indian J Dermatol

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Childhood vitiligo differs from the adults by showing a higher incidence in females, segmental vitiligo being more common and less frequent association with other systemic autoimmune and endocrine disorders. Childhood vitiligo is often associated with a marked psychosocial and long lasting effect on the self-esteem of the affected children and their parents, hence an adequate treatment is very essential. Treatment of vitiligo is indeed a tough challenge for the dermatologists’ more so in the background of childhood vitiligo. Although multiple therapeutic modalities are available in the therapeutic armamentarium, not all can be used in children. This brief report updates regarding various therapies available in the treatment of childhood vitiligo.

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Noncultured epidermal suspension transplantation for the treatment of stable vitiligo in children and adolescents

Cited by 26 publications

References 19 publications

Comparison of intradermal injection of autologous epidermal cell suspension vs. spraying of these cells on dermabraded surface of skin of patients with post-burn hypopigmentation

Comparison of intradermal injection of autologous epidermal cell suspension vs. spraying of these cells on dermabraded surface of skin of patients with post-burn hypopigmentation

Amniotic Membrane as a Scaffold for Melanocyte Transplantation in Patients with Stable Vitiligo

Childhood vitiligo: Treatment paradigms

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