Noncritical phase-matched lithium triborate optical parametric oscillator for high resolution coherent anti-Stokes Raman scattering spectroscopy and microscopy

Jurna, M.; Korterik, Jeroen P.; Offerhaus, Herman L.; Otto, Cornelis

doi:10.1063/1.2420773

Cited by 43 publications

(28 citation statements)

References 15 publications

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“…The laser source (Coherent; Paladin) produces photons of 532 and 1,064 nm. The optical parametric oscillator (APE Berlin) converts photons to longer wavelengths (20). In this setting, the 532-nm photons are converted to 770 nm, serving to visualize the IRDye 800CW bound to the antibody.…”

Section: Ex Vivo Tissue Analysismentioning

confidence: 99%

Intraoperative Near-Infrared Fluorescence Tumor Imaging with Vascular Endothelial Growth Factor and Human Epidermal Growth Factor Receptor 2 Targeting Antibodies

Scheltinga¹,

Dam²,

Nagengast³

et al. 2011

J Nucl Med

177

141

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Fluorescence imaging is currently attracting much interest as a method for intraoperative tumor detection, but most current tracers lack tumor specificity. Therefore, this technique can be further improved by tumor-specific detection. With tumortargeted antibodies bound to a radioactive label, tumor-specific SPECT or PET is feasible in the clinical setting. The aim of the present study was to apply antibody-based tumor detection to intraoperative optical imaging, using preclinical in vivo mouse models. Methods: Anti-vascular endothelial growth factor (VEGF) antibody bevacizumab and anti-human epidermal growth factor receptor (HER) 2 antibody trastuzumab were labeled with the near-infrared (NIR) fluorescence dye IRDye 800CW. Tumor uptake of the fluorescent tracers and their 89 Zr-labeled radioactive counterparts for PET was determined in human xenograft-bearing athymic mice during 1 wk after tracer injection, followed by ex vivo biodistribution and pathologic examination. Intraoperative imaging of fluorescent VEGF-or HER2-positive tumor lesions was performed in subcutaneous tumors and in intraperitoneal dissemination tumor models. Results: Tumorto-background ratios, with fluorescent imaging, were 1.93 6 0.40 for bevacizumab and 2.92 6 0.29 for trastuzumab on day 6 after tracer injection. Real-time intraoperative imaging detected tumor lesions at even the submillimeter level in intraperitoneal dissemination tumor models. These results were supported by standard histology, immunohistochemistry, and fluorescence microscopy analyses. Conclusion: NIR fluorescence-labeled antibodies targeting VEGF or HER2 can be used for highly specific and sensitive detection of tumor lesions in vivo. These preclinical findings encourage future clinical studies with NIR fluorescence-labeled tumor-specific antibodies for intraoperative-guided surgery in cancer patients.

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Section: Ex Vivo Tissue Analysismentioning

confidence: 99%

Intraoperative Near-Infrared Fluorescence Tumor Imaging with Vascular Endothelial Growth Factor and Human Epidermal Growth Factor Receptor 2 Targeting Antibodies

Scheltinga¹,

Dam²,

Nagengast³

et al. 2011

J Nucl Med

177

141

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“…Narrowband CARS provides high resolution detection of molecular transitions [4] and images can be obtained at video rate [5]. The chemical selectivity of CARS microscopy relies on the Raman activity of molecules [6] which gives the CARS signal a unique spectroscopic signature.…”

Section: Introductionmentioning

confidence: 99%

Background free CARS imaging by phase sensitive heterodyne CARS

Jurna¹,

Korterik²,

Otto³

et al. 2008

Opt. Express

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Abstract:In this article we show that heterodyne CARS, based on a controlled and stable phase-preserving chain, can be used to measure amplitude and phase information of molecular vibration modes. The technique is validated by a comparison of the imaginary part of the heterodyne CARS spectrum to the spontaneous Raman spectrum of polyethylene. The detection of the phase allows for rejection of the non-resonant background from the data. The resulting improvement of the signal to noise ratio is shown by measurements on a sample containing lipid.

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“…While power scaling in OPOs depends on the available pump power and optimization of the output coupling [6], rapid tuning relies on the properties of the nonlinear material itself [7]. Several near-infrared OPOs based on birefringent nonlinear crystals such as LiB3O5 (LBO) [2] and KTiOPO4 (KTP) [8,9], and quasi-phase-matched nonlinear materials such as periodically-poled KTP (PPKTP) [3], MgO:sPPLT [10], and MgO:PPLN [11] have been previously demonstrated.…”

mentioning

confidence: 99%

“…Rapid and continuously tunable, high-power, picosecond optical parametric oscillators (OPOs) in the near-infrared wavelength range are highly desirable for the advancement of microscopic techniques such as Coherent Anti-Stokes Raman Scattering (CARS), enabling several applications in biology and medicine [1][2][3]. Such OPOs are typically pumped in the green, which in turn rely on second harmonic generation (SHG) of mode-locked Nd-based solid-state or Yb-based fiber lasers [4].…”

mentioning

confidence: 99%

Fiber-laser-based, green-pumped, picosecond optical parametric oscillator using fan-out grating PPKTP

2015

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We report a stable, Yb-fiber-laser-based, green-pumped, picosecond optical parametric oscillator (OPO) for the near-infrared based on the nonlinear crystal, PPKTP, using fan-out grating design, and operating near room temperature. The OPO is continuously tunable across 726-955 nm in the signal and 1201-1998 nm in the idler, resulting in a total signal plus idler wavelength coverage of 1026 nm by grating tuning at a fixed temperature. The device generates up to 580 mW of average power in the signal at 765 nm and 300 mW in the idler at 1338 nm with an overall extraction efficiency of up to 52% and a pump depletion >76%. The extracted signal at 765 nm and idler at 1746 nm exhibit excellent passive power stability better than 0.5% and 0.8% rms, respectively, over 1 hour, in good beam quality with TEM00 mode profile. The output signal pulses have Gaussian temporal duration of 13.2 ps with a FWHM spectral bandwidth of 3.4 nm at 79.5 MHz repetition rate. Power scaling limitations of the OPO due to the material properties of PPKTP are studied. © 2015 Optical Society of America OCIS codes : (190.4360) Nonlinear optics, devices; (190.7110) Ultrafast nonlinear optics; (190.4400) Nonlinear optics, materials; (190.4970 Rapid and continuously tunable, high-power, picosecond optical parametric oscillators (OPOs) in the near-infrared wavelength range are highly desirable for the advancement of microscopic techniques such as Coherent Anti-Stokes Raman Scattering (CARS), enabling several applications in biology and medicine [1][2][3]. Such OPOs are typically pumped in the green, which in turn rely on second harmonic generation (SHG) of mode-locked Nd-based solid-state or Yb-based fiber lasers [4]. Although picosecond green sources can provide high average powers, the ability to power scale green-pumped picosecond OPOs together with rapid tuning has been challenging [5]. While power scaling in OPOs depends on the available pump power and optimization of the output coupling However, in all these reports wavelength tuning has been achieved by varying the temperature of the nonlinear crystal, which is a relatively slow process.Here we report a stable, picosecond OPO based on fan-out design in PPKTP, enabling rapid grating tuning by translation of the nonlinear crystal while operating close to room temperature. The OPO, which is synchronously pumped by a mode-locked frequency-doubled Yb-fiber laser in the green, can provide continuous tuning over 726-955 nm in the signal wavelength range together with corresponding idler tuning across 1201-1998 nm. A maximum average power of 580 mW at 765 nm in the signal and 300 mW at 1338 nm in the idler, an overall extraction efficiency of up to 52%, and pump depletion >76% have been obtained. To the best of our knowledge, this is the first report of a picosecond OPO based on PPKTP using a fan-out grating design. The schematic of the experimental setup is shown in Fig. 1. The primary pump source is a mode-locked Yb-fiber laser (Fianium, FP1060-20) delivering ~20 ps pulses at 79.5 MHz repet...

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Noncritical phase-matched lithium triborate optical parametric oscillator for high resolution coherent anti-Stokes Raman scattering spectroscopy and microscopy

Cited by 43 publications

References 15 publications

Intraoperative Near-Infrared Fluorescence Tumor Imaging with Vascular Endothelial Growth Factor and Human Epidermal Growth Factor Receptor 2 Targeting Antibodies

Intraoperative Near-Infrared Fluorescence Tumor Imaging with Vascular Endothelial Growth Factor and Human Epidermal Growth Factor Receptor 2 Targeting Antibodies

Background free CARS imaging by phase sensitive heterodyne CARS

Fiber-laser-based, green-pumped, picosecond optical parametric oscillator using fan-out grating PPKTP

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