Noncoding RNAs: Potential players in the self‐renewal of mammalian spermatogonial stem cells

Bie, Beibei; Wang, Ya; Li, Liang; Fang, Huanle; Liu, Libing; Sun, Jin

doi:10.1002/mrd.23041

Cited by 10 publications

(7 citation statements)

References 97 publications

(135 reference statements)

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“…Long ncRNAs (lncRNAs) are important regulatory factors lacking functional Open Reading Frames (ORFs) and localized in both the nucleus and cytoplasm [ 62 ]. Yet, despite the evidence that lncRNAs are important players in diverse cellular processes, the in vivo functional characterization of lncRNA are still experiencing several difficulties [ 63 ]. At this point, in the testis, it is known that some lncRNAs play a critical role in SSC self-renewal, in both humans and mice [ 64 , 65 ], but much is still undisclosed.…”

Section: Epigenetic Signature Of Male Germlinementioning

confidence: 99%

Environmental Impact on Male (In)Fertility via Epigenetic Route

Cescon

Chianese

Tavares

2020

JCM

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In the last 40 years, male reproductive health—which is very sensitive to both environmental exposure and metabolic status—has deteriorated and the poor sperm quality observed has been suggested to affect offspring development and its health in adult life. In this scenario, evidence now suggests that epigenetics shapes endocrine functions, linking genetics and environment. During fertilization, spermatozoa share with the oocyte their epigenome, along with their haploid genome, in order to orchestrate embryo development. The epigenetic signature of spermatozoa is the result of a dynamic modulation of the epigenetic marks occurring, firstly, in the testis—during germ cell progression—then, along the epididymis, where spermatozoa still receive molecules, conveyed by epididymosomes. Paternal lifestyle, including nutrition and exposure to hazardous substances, alters the phenotype of the next generations, through the remodeling of a sperm epigenetic blueprint that dynamically reacts to a wide range of environmental and lifestyle stressors. With that in mind, this review will summarize and discuss insights into germline epigenetic plasticity caused by environmental stimuli and diet and how spermatozoa may be carriers of induced epimutations across generations through a mechanism known as paternal transgenerational epigenetic inheritance.

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Section: Epigenetic Signature Of Male Germlinementioning

confidence: 99%

Environmental Impact on Male (In)Fertility via Epigenetic Route

Cescon

Chianese

Tavares

2020

JCM

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“…Although ncRNAs do not contain an open reading frame (OFR) and cannot transcribe protein, they are involved in the regulation of gene expression [4,5], biological development and metabolic diseases [6,7]. Studies have also shown that ncRNAs act as epigenetic regulators [8] and riboregulators [9], which play vital roles in the early stages and post-transcriptional genetic control of spermatogenesis and oogenesis. These indicate that they can be used as biomarkers for reproductive processes and diseases [10][11][12].…”

Section: Introductionmentioning

confidence: 99%

Long Noncoding RNAs: Recent Insights into Their Role in Male Infertility and Their Potential as Biomarkers and Therapeutic Targets

Zhao

Heng

Sahlu

et al. 2021

IJMS

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Long noncoding RNAs (lncRNAs) are composed of nucleotides located in the nucleus and cytoplasm; these are transcribed by RNA polymerase II and are greater than 200 nt in length. LncRNAs fulfill important functions in a variety of biological processes, including genome imprinting, cell differentiation, apoptosis, stem cell pluripotency, X chromosome inactivation and nuclear transport. As high throughput sequencing technology develops, a substantial number of lncRNAs have been found to be related to a variety of biological processes, such as development of the testes, maintaining the self-renewal and differentiation of spermatogonial stem cells, and regulating spermatocyte meiosis. These indicate that lncRNAs can be used as biomarkers and potential therapeutic targets for male infertility. However, only a few comprehensive reviews have described the role of lncRNAs in male reproduction. In this paper, we summarize recent findings relating to the role of lncRNAs in spermatogenesis, their potential as biomarkers for male infertility and the relationship between reproductive arrest and transgenerational effects. Finally, we suggest specific targets for the treatment of male infertility from the perspective of lncRNAs.

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“…They include microRNAs (miRNAs, 20–25 nt), small interfering RNAs (siRNAs), PIWI-interacting RNAs (piRNAs, 26–31 nt) and circular RNAs (cricRNA). These sncRNAs are essential for several functions such as the regulation of gene expression and genome protection (Ref in Morris and Mattick, 2014 ) as well as during mammalian spermatogenesis ( Yadav and Kotaja, 2014 ; Bie et al, 2018 ; Quan and Li, 2018 ). The second group, lncRNAs, contains transcripts longer than 200 nt without a significant open reading frame.…”

Section: Introductionmentioning

confidence: 99%

Structural and Functional Characterization of a Testicular Long Non-coding RNA (4930463O16Rik) Identified in the Meiotic Arrest of the Mouse Topaz1–/– Testes

Chadourne

Poumerol

Jouneau

et al. 2021

Front. Cell Dev. Biol.

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Spermatogenesis involves coordinated processes, including meiosis, to produce functional gametes. We previously reported Topaz1 as a germ cell-specific gene highly conserved in vertebrates. Topaz1 knockout males are sterile with testes that lack haploid germ cells because of meiotic arrest after prophase I. To better characterize Topaz1–/– testes, we used RNA-sequencing analyses at two different developmental stages (P16 and P18). The absence of TOPAZ1 disturbed the expression of genes involved in microtubule and/or cilium mobility, biological processes required for spermatogenesis. Moreover, a quarter of P18 dysregulated genes are long non-coding RNAs (lncRNAs), and three of them are testis-specific and located in spermatocytes, their expression starting between P11 and P15. The suppression of one of them, 4939463O16Rik, did not alter fertility although sperm parameters were disturbed and sperm concentration fell. The transcriptome of P18-4939463O16Rik–/– testes was altered and the molecular pathways affected included microtubule-based processes, the regulation of cilium movement and spermatogenesis. The absence of TOPAZ1 protein or 4930463O16Rik produced the same enrichment clusters in mutant testes despite a contrasted phenotype on male fertility. In conclusion, although Topaz1 is essential for the meiosis in male germ cells and regulate the expression of numerous lncRNAs, these studies have identified a Topaz1 regulated lncRNA (4930463O16Rik) that is key for both sperm production and motility.

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Noncoding RNAs: Potential players in the self‐renewal of mammalian spermatogonial stem cells

Cited by 10 publications

References 97 publications

Environmental Impact on Male (In)Fertility via Epigenetic Route

Environmental Impact on Male (In)Fertility via Epigenetic Route

Long Noncoding RNAs: Recent Insights into Their Role in Male Infertility and Their Potential as Biomarkers and Therapeutic Targets

Structural and Functional Characterization of a Testicular Long Non-coding RNA (4930463O16Rik) Identified in the Meiotic Arrest of the Mouse Topaz1–/– Testes

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