Noncoding RNAs in oral premalignant disorders and oral squamous cell carcinoma

Huang, Fei; Xin, Chuan; Lei, Kexin; Bai, Hetian; Li, Jing; Chen, Qianming

doi:10.1007/s13402-020-00521-9

Cited by 24 publications

(33 citation statements)

References 125 publications

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“…It is well known that microRNAs induce degradation of target mRNAs or inhibit protein translation via recognizing and binding their 3'-UTR, thereby participating in biological and cancerous processes [19]. A growing number of evidences have shown that microRNAs are involved in the proliferation, apoptosis, migration, metastasis and other events of tumor cells [19,20]. In addition, microRNAs are closely related to drug resistance in cancer cases [21].…”

Section: Ivyspringmentioning

confidence: 99%

Involvement of miR-619-5p in resistance to cisplatin by regulating ATXN3 in oral squamous cell carcinoma

An¹,

Chu²,

Yang³

et al. 2021

Int. J. Biol. Sci.

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MicroRNAs are major post-transcriptional regulators responsible for the development of human cancers, including OSCC. The specific role of miR-619-5p in OSCC, however, is rarely reported. Cisplatin is one of the mostly applied chemotherapy drugs of OSCC. Nevertheless, drug resistance of cisplatin following the initial chemotherapy largely restricts its clinical benefits, and the mechanism of cisplatin resistance is unclear. This study intends to explore the biological function of miR-619-5p in the development of cisplatin resistance in OSCC cell lines and a xenograft model, as well as the potential molecular mechanism. Our results showed that miR-619-5p was down-regulated in OSCC samples and cisplatin-resistant OSCC cells. Ectopically expressed miR-619-5p inhibited proliferative, migratory and invasive abilities of OSCC cisplatin-resistant cells. The putative target gene ATXN3 was predicted by bioinformatic analysis and confirmed by dual-luciferase reporter assay. Importantly, ATXN3 was responsible for the regulatory effects of miR-619-5p on biological behaviors of cisplatin-resistant OSCC cells. Moreover, miR-619-5p mimics and ATXN3-siRNA significantly enhanced ATXN3 knockdown in both HN6/CDDPR and CAL27/CDDPR cells and inhibited expression of PI3K and AKT. In vivo evidences demonstrated that intratumoral injection of miR-619-5p agomir remarkably slowed down the growth of OSCC in xenograft mice. Collectively, microRNA-619-5p was the vital regulator for regulating cisplatin resistance of OSCC, which may be served as a potential therapeutic target.

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Section: Ivyspringmentioning

confidence: 99%

Involvement of miR-619-5p in resistance to cisplatin by regulating ATXN3 in oral squamous cell carcinoma

An¹,

Chu²,

Yang³

et al. 2021

Int. J. Biol. Sci.

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“…According to the most recent report, head and neck squamous cell carcinoma (HNSCC) is a relatively lethal type of cancer, and HNSCC ranks among the top six in terms of incidence and mortality, seriously threatening public health and the quality of life of patients with HNSCC worldwide (1). Despite consecutive forms of treatment, namely, surgery, chemotherapy, and radiotherapy, and considerable advancement in the therapeutic schedule for HNSCC, the 5year survival rate of patients with HNSCC remains far from satisfactory, owing to end-stage diagnosis, rapid development, high recurrence rate, and induction of metastasis to distant sites (2). Mounting evidence has demonstrated that molecular markers hold a promising function not only because of their prognostic value but also because of their role as molecular targets.…”

Section: Introductionmentioning

confidence: 99%

Identification of Expression Patterns and Potential Prognostic Significance of m5C-Related Regulators in Head and Neck Squamous Cell Carcinoma

et al. 2021

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5-Methylcytosine (m5C) methylation is a major epigenetic technique of RNA modification and is dynamically mediated by m5C “writers,” “erasers,” and “readers.” m5C RNA modification and its regulators are implicated in the onset and development of many tumors, but their roles in head and neck squamous cell carcinoma (HNSCC) have not yet been completely elucidated. In this study, we examined expression patterns of core m5C regulators in the publicly available HNSCC cohort via bioinformatic methods. The differentially expressed m5C regulators could divide the HNSCC cohort into four subgroups with distinct prognostic characteristics. Furthermore, a three-gene expression signature model, comprised of NSUN5, DNMT1, and DNMT3A, was established to identify individuals with a high or low risk of HNSCC. To explore the underlying mechanism in the prognosis of HNSCC, screening of differentially expressed genes, followed by the analysis of functional and pathway enrichment, from individuals with high- or low-risk HNSCC was performed. The results revealed a critical role for m5C RNA modification in two aspects of HNSCC: (1) dynamic m5C modification contributes to the regulation of HNSCC progression and (2) expression patterns of NSUN5, DNMT1, and DNMT3A help to predict the prognosis of HNSCC.

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“…A majority of OSCC lesions develop from potentially malignant disorders, and are characterized by invasion, distant metastases, high recurrence rate, and undesirable prognosis. 2 The most common risk factors include smoking, alcohol, and betel nut consumption. 3 For the past few years, therapeutic methods of OSCC, including operative treatment, radiotherapy and chemotherapy, have made great progress.…”

Section: Introductionmentioning

confidence: 99%

Bioinformatics Analyses Indicate That Cathepsin G (CTSG) is a Potential Immune-Related Biomarker in Oral Squamous Cell Carcinoma (OSCC)

Huang¹,

Wu²,

Zheng³

et al. 2021

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Purpose Plenty of studies showed that the immune system was associated with cancer initiation and progression. This study aimed to explore the prognostic biomarkers from immune-related genes (IRGs) in oral squamous cell carcinoma (OSCC). Materials and Methods RNA-seq data were downloaded from The Cancer Genome Atlas (TCGA) and IRGs and transcription factors (TFs) were extracted. Then, the co-expression network between IRGs and TFs was constructed using the “WGCNA” package in R software. Furthermore, a gene expression signature according to IRGs was constructed to predict OSCC prognosis and its accuracy was validated by survival analysis. Subsequently, correlation analyses between risk-score and immune cells level and clinical parameters were performed. Finally, immune-related biomarkers were selected and further investigated using gain-of-function assays in vitro. Results A total of 32 normal cases and 317 OSCC cases were selected in our study. Differentially-expressed analysis indicated that there were 381 differentially-expressed IRGs and 62 TFs in OSCC. Among them, 25 TFs and 21 IRGs were enrolled in the co-expression network. Furthermore, we found that gene expression signature on the basis of 10 IRGs could predict the prognosis accurately and a high-risk score based on gene expression signature meant a high T classification, terminal clinical stage, and low immune cells level in OSCC. Finally, cathepsin G (CTSG) was identified as a potential immune-related biomarker and therapeutic target in OSCC. Conclusion In conclusion, IRGs were directly involved in the development and progression of OSCC. Furthermore, CTSG was identified as a potential independent biomarker and might be an immunotherapeutic target in OSCC treatment.

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Noncoding RNAs in oral premalignant disorders and oral squamous cell carcinoma

Cited by 24 publications

References 125 publications

Involvement of miR-619-5p in resistance to cisplatin by regulating ATXN3 in oral squamous cell carcinoma

Involvement of miR-619-5p in resistance to cisplatin by regulating ATXN3 in oral squamous cell carcinoma

Identification of Expression Patterns and Potential Prognostic Significance of m5C-Related Regulators in Head and Neck Squamous Cell Carcinoma

Bioinformatics Analyses Indicate That Cathepsin G (CTSG) is a Potential Immune-Related Biomarker in Oral Squamous Cell Carcinoma (OSCC)

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