Noncoding RNAs in human intervertebral disc degeneration: An integrated microarray study

Liu, Xu; Che, Lu; Xie, Yan-Ke; Hu, Qing-Jie; Ma, Chi-Jiao; Pei, Yanjun; Wu, Zhigang; Liu, Zhiheng; Fan, Liying; Wang, Hai-Qiang

doi:10.1016/j.gdata.2015.05.027

Cited by 38 publications

(32 citation statements)

References 3 publications

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“…Like miRNAs, lncRNAs are also thought to be major contributors to normal cellular physiological processes and their expression patterns are tissue‐ and cell‐specific . Although studies on lcnRNAs are still in their infancy, they have emerged as critical players in the onset and development of OA and IDD . In this review, we overview the biogenesis, classification and functions of lncRNAs, and focus on their emerging pathological implications and therapeutic potential in IDD and OA.…”

Section: Introductionmentioning

confidence: 99%

lncRNAs: novel players in intervertebral disc degeneration and osteoarthritis

et al. 2016

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The term long non-coding RNA (lncRNA) refers to a group of RNAs with length more than 200 nucleotides, limited protein-coding potential, and having widespread biological functions, including regulation of transcriptional patterns and protein activity, formation of endogenous small interfering RNAs (siRNAs) and natural microRNA (miRNA) sponges. Intervertebral disc degeneration (IDD) and osteoarthritis (OA) are the most common chronic, prevalent and age-related degenerative musculoskeletal disorders. Numbers of lncRNAs are differentially expressed in human degenerative nucleus pulposus tissue and OA cartilage. Moreover, some lncRNAs have been shown to be involved in multiple pathological processes during OA, including extracellular matrix (ECM) degradation, inflammatory responses, apoptosis and angiogenesis. In this review, we summarize current knowledge concerning lncRNAs, from their biogenesis, classification and biological functions to molecular mechanisms and therapeutic potential in IDD and OA. are the most common chronic, prevalent and age-related degenerative musculoskeletal disorders, leading to an enormous socioeconomic burden worldwide. IDD and OA are two major causes of disability and chronic pain, and their incidence has been increasing not only among older persons but also within younger adults in the past decades. It is estimated that approximately 80% adults will suffer chronic low back pain caused by IDD during their lifetime.1 Over 50% of patients with symptomatic OA are younger than 65 years old.

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Section: Introductionmentioning

confidence: 99%

lncRNAs: novel players in intervertebral disc degeneration and osteoarthritis

et al. 2016

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“…GSE63492 was based on an Agilent GPL19449 platform which entitled: Exiqon miRCURY LNA microRNA Array, 7th generation REV-hsa, mmu & rno (miRBase v18.0), submitted by Liu et al (21). The GSE63492 dataset contained 10 samples in total, 5 of which were samples from patients with IDD and the other 5 were tissues from individuals with normal discs.…”

Section: Introductionmentioning

confidence: 99%

MicroRNA-125b-1-3p mediates intervertebral disc degeneration in rats by targeting teashirt zinc finger homeobox 3

et al. 2018

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Abstract. The present study aimed to investigate the association between Teashirt zinc finger homeobox 3 (TSHZ3) and the nucleus pulposus (NP) of intervertebral discs in rats. TSHZ3 was identified from the differentially expressed micro (mi) RNAs in the expression profile of GSE63492 by identifying the overlapped target genes of microRNA (miR)-125b-1-3p across different databases. TSHZ3 small interfering RNA (siRNA) and an miR-125b-1-3p inhibitor were used for gene silencing and gene silencing efficiency was assessed by reverse transcription-polymerase chain reaction. Western blotting was performed to detect the cell cycle proteins cyclin D1 and B1 and the proteins associated with DNA damage in NP. The results revealed that in normal NPs, the expression of TSHZ3 increased following the inhibition of miR-125b-1-3p and in DNA damaged NPs, the expression of TSHZ3 was associated with the degree of DNA damage. The present study demonstrated that TSHZ3, as a target gene of miR-125b-1-3p, may serve a protective role in intervertebral disc degeneration and that this protective function may be inhibited by high levels of miR-125b-1-3p.

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“…184 Notably, expression profiling studies have identified a number of dysregulated miRNAs in human degenerated IVDs when compared to control specimens. [185][186][187][188] Another study reported higher levels of miR-100 in human lumbar degenerated discs compared to traumatized, non-degenerated human specimens and that altered expression levels correlated with FGFR1. 189 A polymorphism in pre-miR-125a (Rs12976445) has been shown to result in a significant reduction in the amount of mature miR-125a by compromising its processing, thereby leading to less efficient inhibition of target genes.…”

Section: Mirnas Associated With Skeletal Disease and Other Orthopaedimentioning

confidence: 99%

MicroRNAs in orthopaedic research: Disease associations, potential therapeutic applications, and perspectives

McAlinden

2017

Journal Orthopaedic Research

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MicroRNAs (miRNAs) are small non-coding RNAs that function to control many cellular processes by their ability to suppress expression of specific target genes. Tens to hundreds of target genes may be affected by one miRNA, thereby resulting in modulation of multiple pathways in any given cell type. Therefore, altered expression of miRNAs (i.e., during tissue development or in scenarios of disease or cellular stress) can have a profound impact on processes regulating cell differentiation, metabolism, proliferation, or apoptosis, for example. Over the past 5-10 years, thousands of reports have been published on miRNAs in cartilage and bone biology or disease, thus highlighting the significance of these non-coding RNAs in regulating skeletal development and homeostasis. For the purpose of this review, we will focus on miRNAs or miRNA families that have demonstrated function in vivo within the context of cartilage, bone or other orthopaedicrelated tissues (excluding muscle). Specifically, we will discuss studies that have utilized miRNA transgenic mouse models or in vivo approaches to target a miRNA with the aim of altering conditions such as osteoarthritis, osteoporosis and bone fractures in rodents. We will not discuss miRNAs in the context skeletal cancers since this topic is worthy of a review of its own. Overall, we aim to provide a comprehensive description of where the field currently stands with respect to the therapeutic potential of specific miRNAs to treat orthopaedic conditions and current technologies to target and modify miRNA function in vivo. KeywordsMicroRNAs (miRNAs); cartilage; bone; skeletal development; osteoarthritis MicroRNA (miRNAs) are small non-coding RNA molecules (typically 19-24 nucleotides in length) that function in RNA silencing and post-transcriptional regulation (suppression) of gene expression. 1 While the majority of miRNAs are located within the cell, some miRNAs, commonly known as circulating miRNA or extracellular miRNA, have also been detected outside the cell in the extracellular matrix, in various biological fluids, or in cell culture. HHS Public Access Author Manuscript Author ManuscriptAuthor ManuscriptAuthor Manuscript miRNAs were first discovered over 20 years ago in the nematode Caenorhabditis elegans with the identification of the developmental regulator lin-4. 3 Since then, thousands of miRNAs have been identified and investigated, with a wide distribution in animals, plants, and viruses. 4 MicroRNAs are ubiquitously expressed in different organisms, and many of them are phylogenetically conserved. 5 To date, over 28,000 miR-NAs from various species are listed in the miRBase website (http://www.mirbase.org). Specifically, 2,588 mature miRNAs have been identified in humans and 1,915 mature miRNAs have been reported in mice.With respect to miRNA biosynthesis, transcription of miRNA genes that are located either intergenically or intragenically is mediated primarily by RNA polymerase II in eukaryotes, although RNA polymerase III has also been shown to transcrib...

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Noncoding RNAs in human intervertebral disc degeneration: An integrated microarray study

Cited by 38 publications

References 3 publications

lncRNAs: novel players in intervertebral disc degeneration and osteoarthritis

lncRNAs: novel players in intervertebral disc degeneration and osteoarthritis

MicroRNA-125b-1-3p mediates intervertebral disc degeneration in rats by targeting teashirt zinc finger homeobox 3

MicroRNAs in orthopaedic research: Disease associations, potential therapeutic applications, and perspectives

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