Noncoding RNAs and pancreatic cancer

Peng, Jintao; Zhuang, Yanyan; Huang, Fengting; Zhang, Shineng

doi:10.3748/wjg.v22.i2.801

Cited by 53 publications

(51 citation statements)

References 148 publications

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“…LncRNAs are believed to regulate gene expression through many mechanisms which have already been reported to be involved in pancreatic cancer [43]. In our study, we predicted the targets of all the LncRNAs in our high throughput sequencing according to the starBase v2.0, AnnoLnc and NONCODE.…”

Section: Panc-1 Spheroid Cells Have Higher Tumorigenic Potential In Vivomentioning

confidence: 99%

Transcriptome Profiling of Panc-1 Spheroid Cells with Pancreatic Cancer Stem Cells Properties Cultured by a Novel 3D Semi-Solid System

Yang¹,

Zhang²,

Tang³

et al. 2018

Cell Physiol Biochem

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Background/Aims: Pancreatic cancer remains one of the deadliest human malignancies, the lethality of which may be attributed to the presence of pancreatic cancer stem cells (PCSCs), a small subpopulation of cells existing within pancreatic tumor with high carcinogenesis. Therefore, it is crucial to establish an efficient enrichment and culture system of PCSCs and identify the key genes involved in the regulation of PCSCs. The three-dimensional (3D) liquid suspension mammosphere culture system has been established for enrichment and culture of PCSCs in vitro as the cell spheres are likely to originate from individual cell clone, but it has been challenged because the cell spheroids could be a result of cell aggregation. Methods: We optimized the existing culture system by adding methylcellulose to create a 3D semi-solid system which prevented the non-specific aggregation. Then we identified the CSC properties of Panc-1 spheroid cells cultured by this system by detecting the genes associated with stemness and by evaluation of the tumorigenicity in vitro and in vivo through invasion, migration and xenograft experiments methods. Subsequently, we performed high-throughput sequencing (HTS) of the Panc-1 spheroid cells. Results: We confirmed the PCSCs properties and high malignancy of the Panc-1 spheroid cells enriched by our novel 3D semi-solid system both in vitro and in vivo. Hundreds of mRNA, microRNA (miRNA) and dozens of long non-coding RNA (LncRNA) were identified to be differentially regulated in PCSCs-like Panc-1 spheroid cells compared with their parental cells by HTS. Conclusions: Our results demonstrate an efficient enrichment and culture system for Panc-1 spheroid cells with the PCSCs properties. The differentially expressed genes and their targets identified by the HTS of the Panc-1 spheroid cells can serve as new potential biomarkers for pancreatic cancer diagnosis and targeted therapy.

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Section: Panc-1 Spheroid Cells Have Higher Tumorigenic Potential In Vivomentioning

confidence: 99%

Transcriptome Profiling of Panc-1 Spheroid Cells with Pancreatic Cancer Stem Cells Properties Cultured by a Novel 3D Semi-Solid System

Yang¹,

Zhang²,

Tang³

et al. 2018

Cell Physiol Biochem

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“…For miR-21 and miR-122, ASOs increase the levels of their targets (PTEN, BCL2, RECK and CDKN1B), reduce proliferation and increase apoptosis of pancreatic cancer cells. In addition, they sensitize PDAC cells to gemcitabine [20,35,39].…”

Section: Mirna and Pancreatic Diseases: Diagnosis Prognosis And Thermentioning

confidence: 99%

“…Oncogene miRNAs stimulate proliferation, angiogenesis and invasion by inhibiting tumor suppressor genes or other genes involved in cell differentiation and apoptosis, and tumor suppressor miRNAs prevent carcinogenesis by negatively inhibiting oncogene or other genes that control cell differentiation and apoptosis. Therefore, the deregulation of miRNA expression (overexpression or underexpression) in cancer leads to the inappropriate expression of its target genes and, consequently, results in initiation, progression and/or invasion of the disease, as well as patient response to chemotherapy (resistance or sensitization) [18][19][20][21].…”

Section: Diagnosismentioning

confidence: 99%

“…Therapeutic applications for miRNAs consist of two strategies: (1) miRNA replacement therapy against loss of function or (2) anti-miRNA therapy against gain of function [20,31,35]. The replacement therapy has the potential to replace malfunctioning tumor suppressor miRNAs and overcome carcinogenesis by reestablishing their normal levels.…”

Section: Mirna and Pancreatic Diseases: Diagnosis Prognosis And Thermentioning

confidence: 99%

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miRNA analysis in pancreatic cancer: the Dartmouth experience

Abreu

Tsongalis

2017

Clinical Chemistry and Laboratory Medicine (CCLM)

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Pancreatic cancer is considered one of the most lethal cancers being the fourth leading cause of cancer deaths in adults in the United States because of the lack of early signs and symptoms and the lack of early detection. Pancreatic ductal adenocarcinoma (PDAC) is the most common histological type among pancreatic cancers, representing 80%-90% of all solid tumors of the pancreas. The majority of PDAC develops from three precursor lesions: pancreatic intraepithelial neoplasia, intraductual papillary mucinous neoplasm and mucinous cystic neoplasm.

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“…miR-200a targets the Keap1 3′-untranslated region, leading to Keap1 mRNA degradation [17, 18]. So far, miRNAs have been studied in pancreatic cancers mainly in vitro, but mounting evidence suggests that they are involved in epithelial-to-mesenchymal transition, cancer stem cell renewal, chemoresistance, and survival in pancreatic cancers [19]. …”

Section: Introductionmentioning

confidence: 99%

Expression Levels of microRNAs miR-93 and miR-200a in Pancreatic Adenocarcinoma with Special Reference to Differentiation and Relapse-Free Survival

et al. 2018

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Objectives: Protein levels of the transcription factor nuclear factor erythroid-derived 2-like 2 (Nrf2) and its inhibitor Kelch-like ECH-associated protein 1 (Keap1) have been proposed as prognostic factors in pancreatic ductal adenocarcinomas (PDACs). These cellular redox-state-regulating enzymes are targeted by several microRNAs, including miR-93 and miR-200a. Methods: We assessed mRNA levels of Nrf2 and Keap1 and tissue expression of miR-93 and miR-200a in 51 patients with surgically treated PDAC. Expression levels were separately measured in malignant cells and adjacent benign cells. Results: Keap1 and Nrf2 mRNA expression levels in cancer cells were lower than in adjacent benign tissue (Wilcoxon’s test; p = 0.0015 and p = 0.000032, respectively). Conversely, miR-93 expression was higher in cancer cells than in adjacent benign tissue (p = 0.00082). Low levels of miR-93 and miR-200a in cancer cells were associated with poorer differentiation (p = 0.004 and p = 0.002, respectively). In univariate survival analysis, benign-tissue levels of miR-200a above the median predicted better relapse-free survival (RFS) (p = 0.045). Conclusions: High miR-93 and miR-200a levels in cancer cells of PDAC were associated with better differentiation, and miR-200a expression in benign tissue with excellent RFS. Keap1 and Nrf2 mRNA levels showed prominent down-regulation in cancerous versus benign tissue, but they were not associated with disease aggressiveness or outcome.

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Noncoding RNAs and pancreatic cancer

Cited by 53 publications

References 148 publications

Transcriptome Profiling of Panc-1 Spheroid Cells with Pancreatic Cancer Stem Cells Properties Cultured by a Novel 3D Semi-Solid System

Transcriptome Profiling of Panc-1 Spheroid Cells with Pancreatic Cancer Stem Cells Properties Cultured by a Novel 3D Semi-Solid System

miRNA analysis in pancreatic cancer: the Dartmouth experience

Expression Levels of microRNAs miR-93 and miR-200a in Pancreatic Adenocarcinoma with Special Reference to Differentiation and Relapse-Free Survival

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