Noncoding RNA of Glutamine Synthetase I Modulates Antibiotic Production in<i>Streptomyces coelicolor</i>A3(2)

D'Alia, Davide; Nieselt, Kay; Steigele, Stephan; Müller, Jean Moritz; Verburg, Ilse; Takano, Eriko

doi:10.1128/jb.01374-09

Cited by 40 publications

(46 citation statements)

References 24 publications

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“…Combinations of bioinformatic predictions and experimental approaches have facilitated the identification of many sRNAs in S. coelicolor and other species (52,(269)(270)(271), and what little is known about their functions points to a boost in the perceived importance of sRNA-mediated regulation of antibiotic production in the near future. Thus, the overexpression of scr5239, an sRNA recently identified in S. coelicolor, decreased ACT production, whereas depletion of scr5239 increased production (272), and overexpression of cnc2198.1as significantly decreased RED production (273). The mechanisms of these effects remain to be understood.…”

Section: Srnasmentioning

confidence: 99%

Molecular Regulation of Antibiotic Biosynthesis in Streptomyces

Liu

Chater

Chandra

et al. 2013

Microbiol Mol Biol Rev

596

536

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SUMMARY Streptomycetes are the most abundant source of antibiotics. Typically, each species produces several antibiotics, with the profile being species specific. Streptomyces coelicolor , the model species, produces at least five different antibiotics. We review the regulation of antibiotic biosynthesis in S. coelicolor and other, nonmodel streptomycetes in the light of recent studies. The biosynthesis of each antibiotic is specified by a large gene cluster, usually including regulatory genes (cluster-situated regulators [CSRs]). These are the main point of connection with a plethora of generally conserved regulatory systems that monitor the organism's physiology, developmental state, population density, and environment to determine the onset and level of production of each antibiotic. Some CSRs may also be sensitive to the levels of different kinds of ligands, including products of the pathway itself, products of other antibiotic pathways in the same organism, and specialized regulatory small molecules such as gamma-butyrolactones. These interactions can result in self-reinforcing feed-forward circuitry and complex cross talk between pathways. The physiological signals and regulatory mechanisms may be of practical importance for the activation of the many cryptic secondary metabolic gene cluster pathways revealed by recent sequencing of numerous Streptomyces genomes.

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Section: Srnasmentioning

confidence: 99%

Molecular Regulation of Antibiotic Biosynthesis in Streptomyces

Liu

Chater

Chandra

et al. 2013

Microbiol Mol Biol Rev

596

536

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“…There are several examples of genes with more than a single asRNA (15,27,60,76), and together with regulatory proteins and other ncRNAs, several input signals can be integrated to adjust the expression of the target gene to the actual need.…”

Section: Other Aspects Of Asrna-based Regulationmentioning

confidence: 99%

cis-Antisense RNA, Another Level of Gene Regulation in Bacteria

Georg

Hess

2011

Microbiol Mol Biol Rev

361

348

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SUMMARY A substantial amount of antisense transcription is a hallmark of gene expression in eukaryotes. However, antisense transcription was first demonstrated in bacteria almost 50 years ago. The transcriptomes of bacteria as different as Helicobacter pylori , Bacillus subtilis , Escherichia coli , Synechocystis sp. strain PCC6803, Mycoplasma pneumoniae , Sinorhizobium meliloti , Geobacter sulfurreducens , Vibrio cholerae , Chlamydia trachomatis , Pseudomonas syringae , and Staphylococcus aureus have now been reported to contain antisense RNA (asRNA) transcripts for a high percentage of genes. Bacterial asRNAs share functional similarities with trans -acting regulatory RNAs, but in addition, they use their own distinct mechanisms. Among their confirmed functional roles are transcription termination, codegradation, control of translation, transcriptional interference, and enhanced stability of their respective target transcripts. Here, we review recent publications indicating that asRNAs occur as frequently in simple unicellular bacteria as they do in higher organisms, and we provide a comprehensive overview of the experimentally confirmed characteristics of asRNA actions and intimately linked quantitative aspects. Emerging functional data suggest that asRNAs in bacteria mediate a plethora of effects and are involved in far more processes than were previously anticipated. Thus, the functional impact of asRNAs should be considered when developing new strategies against pathogenic bacteria and when optimizing bacterial strains for biotechnology.

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“…Cell division trigger factor was also excluded in this study because of a possible requirement for cell growth. 10,11 Changes in growth and antibiotic production by disruption of the identified genes To further analyze the link between the identified proteins and the SAM effect, the corresponding genes (SCO5477, SCO5113, SCO4647, SCO4885 and SCO1793) were disrupted by inserting a fragment of …”

Section: Identification Of Proteins Involved In Sam Signalingmentioning

confidence: 99%

Phosphoprotein affinity purification identifies proteins involved in S-adenosyl-L-methionine-induced enhancement of antibiotic production in Streptomyces coelicolor

et al. 2010

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Streptomycetes are the major natural source of clinical antibiotics. The enhanced secondary metabolite production of many streptomycetes by S-adenosylmethionine (SAM) in previous studies suggested the existence of a common SAM regulatory effect. We screened nine proteins using the phosphoprotein purification column from Streptomyces coelicolor. Among them, genes (SCO5477, SCO5113, SCO4647, SCO4885 and SCO1793) for five proteins were disrupted by insertion mutation. The undecylprodigiosin and actinorhodin productions were changed in all mutations. The SAM-induced enhancement of actinorhodin production was abolished by all mutations except SCO4885 mutation, which reduced the production of actinorhodin and undecylprodigiosin with SAM treatment. This study demonstrates that phosphoprotein affinity purification can be used as a screening method to identify the proteins involved SAM signaling. Keywords: actinorhodin; protein phosphorylation; S-adenosylmethionine; Streptomyces coelicolor; undecylprodigiosin INTRODUCTIONStreptomyces is the major genus that produces useful antibiotics of natural origin. Secondary metabolite production in Streptomyces is usually strain specific; thus, it is difficult to develop a universal method to promote the production of secondary metabolites in most Streptomyces strains. 1,2 We observed that increased concentration of S-adenosylmethionine (SAM) by overexpression of SAM synthetase gene stimulated actinorhodin production in Streptomyces lividans, 3,4 and exogenous SAM treatment in culture media enhanced streptomycin production in S. griseus, 5 as well as other secondary metabolite production in many Streptomyces strains. 6 On the basis of these results, we sought to identify SAM-response proteins, which might be targets, to improve secondary metabolite production in many Streptomyces spp.SAM-enhanced secondary metabolite production in all tested actinomycetes suggested the existence of a common signal transduction pathway. To identify cellular proteins that regulate the SAM response, we hypothesized that changes in protein phosphorylation constitute part of the signal transduction of SAM responses in S. coelicolor, a model strain for actinomycetes. As a new approach to

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Noncoding RNA of Glutamine Synthetase I Modulates Antibiotic Production inStreptomyces coelicolorA3(2)

Cited by 40 publications

References 24 publications

Molecular Regulation of Antibiotic Biosynthesis in Streptomyces

Molecular Regulation of Antibiotic Biosynthesis in Streptomyces

cis-Antisense RNA, Another Level of Gene Regulation in Bacteria

Phosphoprotein affinity purification identifies proteins involved in S-adenosyl-L-methionine-induced enhancement of antibiotic production in Streptomyces coelicolor

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