Noncoding Flavivirus RNA Displays RNA Interference Suppressor Activity in Insect and Mammalian Cells

Schnettler, Esther; Sterken, Mark G.; Leung, Jason Y.; Metz, Stefan; Geertsema, Corrine; Goldbach, Rob; Vlak, Just M.; Kohl, Alaine; Khromykh, Alexander A.; Pijlman, Gorben P.

doi:10.1128/jvi.01104-12

Cited by 255 publications

(276 citation statements)

References 75 publications

(133 reference statements)

Supporting

Mentioning

267

Contrasting

Unclassified

Order By: Relevance

“…In fact, evidence suggests that there are significant differences between the different arbovirus families which need to be taken into consideration. Some (or possibly all) flaviviruses have the ability to suppress RNAi [59,101]; thus it is not surprising that other innate immunity pathways such as JAK/STAT and Toll are involved in controlling flavivirus infection in mosquitoes [92,115,130]. In contrast, alphaviruses are not known to actively suppress RNAi and use only decoy mechanisms to evade the RNAi response [111].…”

Section: Discussionmentioning

confidence: 99%

“…The only arboviruses known to encode suppressors of RNAi are flaviviruses. WNV encodes a subgenomic RNA in the 3 0 untranslated region of its genome (subgenomic flaviviral RNA) which acts as an RNAi suppressor in both mammalian and insect cells [101]. The NS4B protein of another flavivirus, DENV-2, has also been shown to act as a suppressor of mammalian and insect RNAi [59].…”

Section: Mirnasmentioning

confidence: 99%

See 1 more Smart Citation

Antiviral responses of arthropod vectors: an update on recent advances

et al. 2014

View full text Add to dashboard Cite

Arthropod vectors, such as mosquitoes, ticks, biting midges and sand flies, transmit many viruses that can cause outbreaks of disease in humans and animals around the world. Arthropod vector species are invading new areas due to globalisation and environmental changes, and contact between exotic animal species, humans and arthropod vectors is increasing, bringing with it the regular emergence of new arboviruses. For future strategies to control arbovirus transmission, it is important to improve our understanding of virus-vector interactions. In the last decade knowledge of arthropod antiviral immunity has increased rapidly. RNAi has been proposed as the most important antiviral response in mosquitoes and it is likely to be the most important antiviral response in all arthropods. However, other newly-discovered antiviral strategies such as melanisation and the link between RNAi and the JAK/STAT pathway via the cytokine Vago have been characterised in the last few years. This review aims to summarise the most important and most recent advances made in arthropod antiviral immunity.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Mirnasmentioning

confidence: 99%

Antiviral responses of arthropod vectors: an update on recent advances

et al. 2014

View full text Add to dashboard Cite

show abstract

“…The mechanism of this reduction is unknown but competitive inhibition as well as induction of the RNA interference (RNAi) response are possible explanations. 8,[42][43][44] Regardless of the mechanism, infection with ISFs is another example of how a mosquito's microbiome can affect its vector competence for certain arboviruses. The effect of the endosymbiotic bacterium Wolbachia on the vector competence of Aedes aegypti for dengue virus and several other arboviruses has been well documented.…”

Section: Discussionmentioning

confidence: 99%

Mercadeo Virus: A Novel Mosquito-Specific Flavivirus from Panama

Carrera¹,

Guzmán²,

Beltran³

et al. 2015

The American Journal of Tropical Medicine and Hygiene

View full text Add to dashboard Cite

Abstract. Viruses in the genus Flavivirus (family Flaviviridae) include many arthropod-borne viruses of public health and veterinary importance. However, during the past two decades an explosion of novel insect-specific flaviviruses (ISFs), some closely related to vertebrate pathogens, have been discovered. Although many flavivirus pathogens of vertebrates have been isolated from naturally infected mosquitoes in Panama, ISFs have not previously been reported from the country. This report describes the isolation and characterization of a novel ISF, tentatively named Mercadeo virus (MECDV), obtained from Culex spp. mosquitoes collected in Panama. Two MECDV isolates were sequenced and cluster phylogenetically with cell-fusing agent virus (CFAV) and Nakiwogo virus (NAKV) to form a distinct lineage within the insect-specific group of flaviviruses.

show abstract

“…31 This sfRNA has also been shown to modulate host responses to infection via subversion of the interferon response, 32 inhibition of cellular exoribonuclease, 33 and suppression of the antiviral RNA interference response. 34 However, the specific binding partners for sfRNA have not been elucidated. 30 Multiple amino acid sequence alignments of the core protein of members of the Flaviviridae combined with structural analysis demonstrate conservation within the N-terminal region.…”

Section: Other Members Of the Flaviviridae May Be Capable Of Inducingmentioning

confidence: 99%

The Impact of Prior Flavivirus Infections on the Development of Type 2 Diabetes Among the Indigenous Australians

Sorenson

Owens

Caltabiano

et al. 2016

The American Journal of Tropical Medicine and Hygiene

View full text Add to dashboard Cite

Abstract. It is estimated that 5% of Australians over the age of 18 have diabetes, with the number of new cases increasing every year. Type 2 diabetes (T2D) also represents a significant disease burden in the Australian indigenous population, where prevalence is three times greater than that of non-indigenous Australians. Prevalence of T2D has been found to be higher in rural and remote indigenous Australian populations compared with urban indigenous Australian populations. Several studies have also found that body mass index and waist circumference are not appropriate for the prediction of T2D risk in indigenous Australians. Regional and remote areas of Australia are endemic for a variety of mosquito-borne flaviviruses. Studies that have investigated seroprevalence of flaviviruses in remote aboriginal communities have found high proportions of seroconversion. The family Flaviviridae comprises several genera of viruses with nonsegmented single-stranded positive sense RNA genomes, and includes the flaviviruses and hepaciviruses. Hepatitis C virus (HCV) has been shown to be associated with insulin resistance and subsequent development of T2D. Flaviviruses and HCV possess conserved proteins and subgenomic RNA structures that may play similar roles in the development of insulin resistance. Although dietary and lifestyle factors are associated with increased risk of developing T2D, the impact of infectious diseases such as arboviruses has not been assessed. Flaviviruses circulating in indigenous Australian communities may play a significant role in inducing glucose intolerance and exacerbating T2D. DIABETES MELLITUS TYPE 2 IN INDIGENOUS AUSTRALIANSChronic disease is the epidemic of the new millennium, surpassing infectious disease and injury as the dominant health concern currently facing humanity. It is estimated that, by 2020, 75% of deaths in Australia will be due to chronic diseases, such as diabetes.1 A systematic review of type 2 diabetes (T2D) studies surveying both rural and urban indigenous Australian populations found higher prevalence of T2D in the former compared with the latter.2 This result differs from prevalence surveys conducted with indigenous peoples in North America and the Pacific.2 The onset of T2D in nonindigenous Australians generally occurs in the mid-forties, with prevalence peaking in the age of 75 years and older cohort.2 For indigenous Australians, prevalence is highest in the 35-to 55-year old group. 2 The prevalence of T2D within the indigenous Australian community is three times higher than non-indigenous Australians.3 Development of T2D is predominantly associated with metabolic changes due to obesity, and the increase in T2D in indigenous Australians is primarily attributed to changes in nutrition. However, urbanliving indigenous Australians have similar access to a nontraditional diet and it would be expected they would have similar prevalence of T2D. In addition, several studies on the outcomes of educational and dietary intervention initiatives in remote communities have demonstra...

show abstract

Noncoding Flavivirus RNA Displays RNA Interference Suppressor Activity in Insect and Mammalian Cells

Cited by 255 publications

References 75 publications

Antiviral responses of arthropod vectors: an update on recent advances

Antiviral responses of arthropod vectors: an update on recent advances

Mercadeo Virus: A Novel Mosquito-Specific Flavivirus from Panama

The Impact of Prior Flavivirus Infections on the Development of Type 2 Diabetes Among the Indigenous Australians

Contact Info

Product

Resources

About