Nonalcoholic Fatty Gallbladder Disease: The Influence of Diet in Lean and Obese Mice

Goldblatt, Matthew I.; Swartz-Basile, Deborah A.; Al-Azzawi, Hayder H.; Tran, Khoi Q.; Nakeeb, Attila; Pitt, Henry A.

doi:10.1016/j.gassur.2005.07.009

Cited by 27 publications

(22 citation statements)

References 34 publications

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“…Pancreatic lipid content was determined by thin-layer and gas chromatography as previously described [2] . Briefly, lipids were extracted by the method of Folch-Lees.…”

Section: Lipid Analysis By Thin-layer and Gas Chromatographymentioning

confidence: 99%

“…Individual lipid classes were separated by thin-layer chromatography using Silica Gel 60 A plates and visualized by rhodamine 6G. In addition, total cholesterol was analyzed by the method of Goldblatt et al [2] . An aliquot of the Folch extract was saponified with 1 N KOH in 90% methanol.…”

Section: Lipid Analysis By Thin-layer and Gas Chromatographymentioning

confidence: 99%

“…The burgeoning obesity epidemic in the United States has focused a spotlight on the role of adipose tissue in the multiple pathologic effects of obesity [1][2][3][4] . Adipose tissue secretes a number of proteins collectively referred to as adipokines that are important modulators of metabolism, inflammation and energy intake [5] .…”

Section: Introductionmentioning

confidence: 99%

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Nuclear Magnetic Resonance Spectroscopy-Based Metabolomics of the Fatty Pancreas: Implicating Fat in Pancreatic Pathology

et al. 2009

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Background: Obesity is a worldwide epidemic and a significant risk factor for pancreatic diseases including pancreatitis and pancreatic cancer; the mechanisms underlying this association are unknown. Metabolomics is a powerful new analytical approach for describing the metabolome (compliment of small molecules) of cells, tissue or biofluids at any given time. Our aim was to analyze pancreatic fat content in lean and congenitally obese mice using both metabolomic analysis and conventional chromatography. Methods: The pancreatic fat content of 12 lean (C57BL/6J), 12 obese leptin-deficient (Lep^ob) and 12 obese hyperleptinemic (Lep^db) mice was evaluated by metabolomic analysis, thin-layer and gas chromatography. Results: Pancreata of congenitally obese mice had significantly more total pancreatic fat, triglycerides and free fatty acids, but significantly less phospholipids and cholesterol than those of lean mice. Metabolomic analysis showed excellent correlation with thin-layer and gas chromatography in measuring total fat, triglycerides and phospholipids. Conclusions: Differences in pancreatic fat content and character may have important implications when considering the local pancreatic proinflammatory milieu in obesity. Metabolomic analysis is a valid, powerful tool with which to further define the mechanisms by which fat impacts pancreatic disease.

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“…Pancreatic lipid content was determined by thin-layer and gas chromatography as previously described [2] . Briefly, lipids were extracted by the method of Folch-Lees.…”

Section: Lipid Analysis By Thin-layer and Gas Chromatographymentioning

confidence: 99%

Section: Lipid Analysis By Thin-layer and Gas Chromatographymentioning

confidence: 99%

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Nuclear Magnetic Resonance Spectroscopy-Based Metabolomics of the Fatty Pancreas: Implicating Fat in Pancreatic Pathology

et al. 2009

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“…Obesity related fatty infiltration of the gallbladder leading to both structural and functional damage has been shown in animal studies. 21,22 One of the reasons of a possible relation between fatty GD and GD is the lipotoxicity caused by ectopic lipid accumulation in non-adipose tissues. The increase in cholesterol/phospholipid ratio in the muscle cells of the gallbladder due to this lipotoxicity decreases muscle contractility directly, and also decreases the humoral response to neurotransmitters like acetylcholine, cholecystokinin, and neuropeptide Y.…”

mentioning

confidence: 99%

“…The increase in cholesterol/phospholipid ratio in the muscle cells of the gallbladder due to this lipotoxicity decreases muscle contractility directly, and also decreases the humoral response to neurotransmitters like acetylcholine, cholecystokinin, and neuropeptide Y. [22][23][24][25][26] An important animal study on this subject has shown that ezetimibe, a cholesterol lowering agent limiting intestinal absorption of dietary and gallbladder originated cholesterol, both decreases lipid quantity of gallbladder wall and ameliorates gallbladder functions. 27 It also showed a positive effect on fatty GD by decreasing crystallization.…”

mentioning

confidence: 99%

Impaired Gallbladder Motility and Increased Gallbladder Wall Thickness in Patients with Nonalcoholic Fatty Liver Disease

Çolak

Bozbey

Erim

et al. 2016

J Neurogastroenterol Motil

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Background/AimsNonalcoholic fatty liver disease (NAFLD) is currently the most common chronic liver disease worldwide. Along with the increase in the incidence of NAFLD and associated obesity, an increase in gallbladder disease (GD) has been noted. This has led to the identification of a new disease entity called fatty GD. There is a gap in the literature on the dynamics of gallbladder function in patients with NAFLD. MethodsAn observational case-control study, a total of 50 patients with biopsy proven NAFLD without gallbladder stone/sludge and 38 healthy comparison subjects were enrolled. Fasting, postprandial gallbladder volumes (PGV), gallbladder ejection fraction (GEF), and fasting gallbladder wall thickness (FGWT) were measured by real-time 2-dimensional ultrasonography. ResultsFasting gallbladder wall thickness, fasting gallbladder volumes and PGV were significantly higher in patients with NAFLD than control subjects (P < 0.001, P = 0.006, and P < 0.001, respectively). Gallbladder ejection fraction was significantly lower in the NAFLD group than the controls (P = 0.008). The presence of NAFLD was an independent predictor for GEF, PGV, and FGWT. Also, steatosis grade was an independent predictor for GEF, and GEF was significantly lower in the nonalcoholic steatohepatitis (NASH) subgroup than the controls. ConclusionsGallbladder dysfunction and increase in gallbladder wall thickness exists in asymptomatic (without stone/sludge and related symptoms) patients with NAFLD and are useful in identifying fatty GD. Measurement of these variables in NAFLD patients may be useful in identifying those at higher risk for GD.

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Acute Biliary Tract Diseases

Augustin

2014

Acute Abdomen During Pregnancy

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Nonalcoholic Fatty Gallbladder Disease: The Influence of Diet in Lean and Obese Mice

Cited by 27 publications

References 34 publications

Nuclear Magnetic Resonance Spectroscopy-Based Metabolomics of the Fatty Pancreas: Implicating Fat in Pancreatic Pathology

Nuclear Magnetic Resonance Spectroscopy-Based Metabolomics of the Fatty Pancreas: Implicating Fat in Pancreatic Pathology

Impaired Gallbladder Motility and Increased Gallbladder Wall Thickness in Patients with Nonalcoholic Fatty Liver Disease

Acute Biliary Tract Diseases

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