Nonadhesive populations in cultures of mesenchymal stromal cells from hematopoietic organs in mouse and rat

Bueverova, E. I.; Bragina, E. Yu.; Молчанова, Е. А.

doi:10.1134/s1062360408060027

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2012

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“…This procedure was repeated after 2 hours, and, 15-60 min later, the cultures were ana lyzed for the kinetics of protein synthesis. Some exper iments were performed with MSC from the rat bone marrow (Bueverova et al, 2008).…”

Section: Methodsmentioning

confidence: 99%

Mesenchymal stromal cells synchronize the rhythm of protein synthesis under the effect of an exogenous signal

et al. 2012

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A comparative study was performed of dense 5 hour cultures of rat hepatocytes and equal density cultures of mesenchymal stromal cells (MSC) isolated from human adipose tissue of rat bone marrow. The cells were grown on collagen coated class slides in serum free medium. Unlike in hepatocytes, no rhythm of protein synthesis was initially revealed in MSC, but such a rhythm manifested itself when the culture medium was supplemented with melatonin (2 nM, 5 min). The results of experiments with cytoplasmic calcium che lator BAPTA AM and protein kinase inhibitor H7 indicate that the mechanism of protein synthesis synchro nization in MSC consists in calcium dependent phosphorylation of cell proteins.

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Section: Methodsmentioning

confidence: 99%