Non-viral Smad7 gene delivery and attenuation of postoperative peritoneal adhesion in an experimental model

Abstract

Ultrasound-microbubble-mediated gene transfection provides timely targeted gene delivery for the treatment of postoperative peritoneal adhesions.

Variation in stability of housekeeping genes in healthy and adhesion-related mesothelium

“…Our study demonstrates that the frequently used HKG, that is, GAPDH, is not suitable for normalization of adhesion tissue and therefore could account for the variation of results obtained by these studies (21)(22)(23). Our study shows that GAPDH expression is unstable across tissue types, a finding that is consistent with other studies (24).…”

Visceral mobilization can lyse and prevent peritoneal adhesions in a rat model

“…Prevention is a far more desirable goal (Gomel et al, 1996;Mettler, 2003;Scott-Coombes et al, 1993), and much effort has been expended on adhesion prevention using various chemical compounds and barriers (Corrales et al, 2008;Guo et al, 2009;Ilbay et al, 2004;Kutlay et al, 2004;Mettler et al, 2003;Oncel et al, 2004). Systematic reviews of these treatments have reported that none consistently prevent adhesion formation (Kumar et al, 2009;Metwally et al, 2006).…”

Mesenchymal Stem Cells Attenuate Peritoneal Injury through Secretion of TSG-6

“…No satisfactory solution has proved to ameliorate peritoneal fibrosis efficiently. Recent studies have shown that the recovery of surgically damaged mesothelium reduces the peritoneal adhesions and fibrosis, resulting in improved structural repair and function of peritoneum [6], [8]. Therefore, promoting the mesothelial recovery may be a novel therapeutic target to reduce peritoneal fibrosis.…”