Non-unions

Calori, GM

doi:10.11138/ccmbm/2017.14.1.186

Cited by 74 publications

(43 citation statements)

References 21 publications

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“…Alveolar bone resorption due to periodontal disease may cause loss of teeth, potentially resulting in systemic infection, eating disturbance, malnutrition, and dementia 1 , 2 . Autologous bone graft has been performed to treat a patient with severe bone loss, but the preparation of a sufficient amount of bone for transplantation may cause adverse events such as pain in the patients at the sacrificed donor site 3 . In this context, an efficient regenerative therapy to treat bone loss and bone resorption disorders is strongly needed.…”

Section: Introductionmentioning

confidence: 99%

Nanogel tectonic porous 3D scaffold for direct reprogramming fibroblasts into osteoblasts and bone regeneration

Satô

Yamamoto

Horiguchi

et al. 2018

Sci Rep

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Transplantation of engineered three-dimensional (3D) bone tissue may provide therapeutic benefits to patients with various bone diseases. To achieve this goal, appropriate 3D scaffolds and cells are required. In the present study, we devised a novel nanogel tectonic material for artificial 3D scaffold, namely the nanogel-cross-linked porous (NanoCliP)-freeze-dried (FD) gel, and estimated its potential as a 3D scaffold for bone tissue engineering. As the osteoblasts, directly converted osteoblasts (dOBs) were used, because a large number of highly functional osteoblasts could be induced from fibroblasts that can be collected from patients with a minimally invasive procedure. The NanoCliP-FD gel was highly porous, and fibronectin coating of the gel allowed efficient adhesion of the dOBs, so that the cells occupied the almost entire surface of the walls of the pores after culturing for 7 days. The dOBs massively produced calcified bone matrix, and the culture could be continued for at least 28 days. The NanoCliP-FD gel with dOBs remarkably promoted bone regeneration in vivo after having been grafted to bone defect lesions that were artificially created in mice. The present findings suggest that the combination of the NanoCliP-FD gel and dOBs may provide a feasible therapeutic modality for bone diseases.

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Section: Introductionmentioning

confidence: 99%

Nanogel tectonic porous 3D scaffold for direct reprogramming fibroblasts into osteoblasts and bone regeneration

Satô

Yamamoto

Horiguchi

et al. 2018

Sci Rep

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“…Functional hydrogels are one possible biomaterial choice that were recently developed to promote bone tissue regeneration. Hydrogel scaffolds, in particular, have been demonstrated to boost cell adhesion [ 5 , 6 ].…”

Section: Introductionmentioning

confidence: 99%

In Vivo Regeneration of Large Bone Defects by Cross-Linked Porous Hydrogel: A Pilot Study in Mice Combining Micro Tomography, Histological Analyses, Raman Spectroscopy and Synchrotron Infrared Imaging

et al. 2020

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The transplantation of engineered three-dimensional (3D) bone graft substitutes is a viable approach to the regeneration of severe bone defects. For large bone defects, an appropriate 3D scaffold may be necessary to support and stimulate bone regeneration, even when a sufficient number of cells and cell cytokines are available. In this study, we evaluated the in vivo performance of a nanogel tectonic 3D scaffold specifically developed for bone tissue engineering, referred to as nanogel cross-linked porous-freeze-dry (NanoCliP-FD) gel. Samples were characterized by a combination of micro-computed tomography scanning, Raman spectroscopy, histological analyses, and synchrotron radiation–based Fourier transform infrared spectroscopy. NanoCliP-FD gel is a modified version of a previously developed nanogel cross-linked porous (NanoCliP) gel and was designed to achieve highly improved functionality in bone mineralization. Spectroscopic imaging of the bone tissue grown in vivo upon application of NanoCliP-FD gel enables an evaluation of bone quality and can be employed to judge the feasibility of NanoCliP-FD gel scaffolding as a therapeutic modality for bone diseases associated with large bone defects.

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“…The process of bone healing occurs appropriately in the majority of cases through the formation of callus . However, some fractures fail to heal which can result in delayed union or persistent non‐union and prolonged disability . There are various causes of failed bone repair including inadequate blood supply, inappropriate angiogenesis, insufficient immobilization and infection .…”

Section: Introductionmentioning

confidence: 99%

EPC‐derived exosomes promote osteoclastogenesis through LncRNA‐MALAT1

Cui

Sun

et al. 2019

J Cellular Molecular Medi

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Bone repair involves bone resorption through osteoclastogenesis and the stimulation of neovascularization and osteogenesis by endothelial progenitor cells ( EPC s). However, the role of EPC s in osteoclastogenesis is unclear. In this study, we assess the effects of EPC ‐derived exosomes on the migration and osteoclastic differentiation of primary mouse bone marrow‐derived macrophages ( BMM s) in vitro using immunofluorescence, western blotting, RT ‐ PCR and Transwell assays. We also evaluated the effects of EPC ‐derived exosomes on the homing and osteoclastic differentiation of transplanted BMM s in a mouse bone fracture model in vivo. We found that EPC s cultured with BMM s secreted exosomes into the medium and, compared with EPC s, exosomes had a higher expression level of Lnc RNA ‐ MALAT 1. We confirmed that Lnc RNA ‐ MALAT 1 directly binds to miR‐124 to negatively control miR‐124 activity. Moreover, overexpression of miR‐124 could reverse the migration and osteoclastic differentiation of BMM s induced by EPC ‐derived exosomes. A dual‐luciferase reporter assay indicated that the integrin ITGB 1 is the target of miR‐124. Mice treated with EPC ‐derived exosome‐ BMM co‐transplantations exhibited increased neovascularization at the fracture site and enhanced fracture healing compared with those treated with BMM s alone. Overall, our results suggest that EPC ‐derived exosomes can promote bone repair by enhancing recruitment and differentiation of osteoclast precursors through Lnc RNA ‐ MALAT 1.

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Non-unions

Cited by 74 publications

References 21 publications

Nanogel tectonic porous 3D scaffold for direct reprogramming fibroblasts into osteoblasts and bone regeneration

Nanogel tectonic porous 3D scaffold for direct reprogramming fibroblasts into osteoblasts and bone regeneration

In Vivo Regeneration of Large Bone Defects by Cross-Linked Porous Hydrogel: A Pilot Study in Mice Combining Micro Tomography, Histological Analyses, Raman Spectroscopy and Synchrotron Infrared Imaging

EPC‐derived exosomes promote osteoclastogenesis through LncRNA‐MALAT1

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