“…All samples were investigated with Yfiler 1 Plus according to the manufacturer's protocol with modifications in the reaction volume (12.5 ml), the volume of input DNA (0.5 ml) and the number of PCRcycles (25)(26)(27)(28)(29). Prior to electrophoresis, 1 ml of the amplified products and 0.5 ml of GeneScan TM 600 LIZ 1 Size Standard v. 2.0 were added to 9.5 ml of deionized Hi-Di TM formamide and denatured for 3 min at 95 C. The samples were electrophoresed on an Applied Biosystems 1 3500 Â L Genetic Analyzer (Thermo Fisher Scientific, Waltham, MA, USA) according to the recommendations of the manufacturer except for a slight modification of the injection time (12 s).…”