Non-Uniform Distribution Pattern for Differentially Expressed Genes of Transgenic Rice Huahui 1 at Different Developmental Stages and Environments

Zhi, Liu; Zhao, Jie; Li, Yunhe; Zhang, Wenwei; Jian, Guiliang; Peng, Yufa; Qi, Fangjun

doi:10.1371/journal.pone.0037078

Cited by 12 publications

(9 citation statements)

References 25 publications

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“…The transcriptomic comparisons result indicated that the expressed levels of all these six genes can be regulated by biotic or abiotic stresses in previous studies. The result of study (GSE33204) indicated that Os02g0527300 (OsHsfA3) and Os03g0335200 (OsWRKY44) showed upregulation, and showed downregulation in 30-day-old leaves of rice after 6H high temperature treatment [39]. However, these three genes did not show alternated expression level in WT induced by heat stress in our study, which might be attributed to the different genotype, development stage, tissue or treatment condition.…”

Section: Atplc9 Confers Heat Tolerance To Monocotyledons As Well As Dcontrasting

confidence: 55%

Heterologous expression of heat stress-responsive AtPLC9 confers heat tolerance in transgenic rice

Liu¹,

Liu²,

Wang³

et al. 2020

BMC Plant Biol

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Background As global warming becomes increasingly severe, it is urgent that we enhance the heat tolerance of crops. We previously reported that Arabidopsis thaliana PHOSPHOINOSITIDE-SPECIFIC PHOSPHOLIPASE C9 (AtPLC9) promotes heat tolerance. Results In this study, we ectopically expressed AtPLC9 in rice to examine its potential to improve heat tolerance in this important crop. Whereas AtPLC9 did not improve rice tolerance to salt, drought or cold, transgenic rice did exhibit greater heat tolerance than the wild type. High-throughput RNA-seq revealed extensive and dynamic transcriptome reprofiling in transgenic plants after heat stress. Moreover, the expression of some transcription factors and calcium ion-related genes showed specific upregulation in transgenic rice after heat stress, which might contribute to the enhanced heat tolerance. Conclusions This study provides preliminary guidance for using AtPLC9 to improve heat tolerance in cereal crops and, more broadly, highlights that heterologous transformation can assist with molecular breeding.

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Section: Atplc9 Confers Heat Tolerance To Monocotyledons As Well As Dcontrasting

confidence: 55%

Heterologous expression of heat stress-responsive AtPLC9 confers heat tolerance in transgenic rice

Liu¹,

Liu²,

Wang³

et al. 2020

BMC Plant Biol

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“…T2A‐1 expresses a synthetic cry2A gene and T1C‐19b expresses a synthetic cry1C gene, with expression of both driven by the maize ubiquitin promoter (Wang et al ., ; Li et al ., ; Li et al ., ). Huahui 1 expresses a fused cry1Ab/Ac gene under the control of rice actinI promoter (Liu et al ., ). Minghui 63 is an elite indica restorer line for cytoplasmic male sterility in China, and served as non‐transgenic control in this study.…”

Section: Methodsmentioning

confidence: 97%

Comparison of three transgenic Bt rice lines for insecticidal protein expression and resistance against a target pest, Chilo suppressalis (Lepidoptera: Crambidae)

Wang

et al. 2015

Insect Science

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Two transgenic rice lines (T2A-1 and T1C-19b) expressing cry2A and cry1C genes, respectively, were developed in China, targeting lepidopteran pests including Chilo suppressalis (Walker) (Lepidoptera: Crambidae). The seasonal expression of Cry proteins in different tissues of the rice lines and their resistance to C. suppressalis were assessed in comparison to a Bt rice line expressing a cry1Ab/Ac fusion gene, Huahui 1, which has been granted a biosafety certificate. In general, levels of Cry proteins were T2A-1 > Huahui 1 > T1C-19b among rice lines, and leaf > stem > root among rice tissues. The expression patterns of Cry protein in the rice line plants were similar: higher level at early stages than at later stages with an exception that high Cry1C level in T1C-19b stems at the maturing stage. The bioassay results revealed that the three transgenic rice lines exhibited significantly high resistance against C. suppressalis larvae throughout the rice growing season. According to Cry protein levels in rice tissues, the raw and corrected mortalities of C. suppressalis caused by each Bt rice line were the highest in the seedling and declined through the jointing stage with an exception for T1C-19b providing an excellent performance at the maturing stage. By comparison, T1C-19b exhibited more stable and greater resistance to C. suppressalis larvae than T2A-1, being close to Huahui 1. The results suggest cry1C is an ideal Bt gene for plant transformation for lepidopteran pest control, and T1C-19b is a promising Bt rice line for commercial use for tolerating lepidopteran rice pests.

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“…Previous studies have shown that comparisons of differences in agronomic traits ( Xia et al, 2011 ; Wang et al, 2012a ; Li et al, 2013 ; Jiang et al, 2016 ), nutritional composition ( Wang et al, 2012b ; Jian et al, 2014 ; Jiang et al, 2018 ; Takeda et al, 2019 ), environmental adaptability ( Jiang et al, 2018 ; Wang et al, 2019 ; Park et al, 2019 ), gene transcription, protein expression, and metabolite levels ( Liu et al, 2012 ; Liu Y. et al, 2015 ; Fu et al, 2019 ; Peng et al, 2019 ) among transgenic crops and their parents are important means and strategies for confirming and excluding the unintended effects of transgenic crops, in addition to facilitating any safety evaluations. However, the molecular mechanisms of underlying the unintended effects observed in transgenic crops are unclear.…”

Section: Discussionmentioning

confidence: 99%

Exogenous Cry1Ab/c Protein Recruits Different Endogenous Proteins for Its Function in Plant Growth and Development

Liu

2020

Front. Bioeng. Biotechnol.

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Current risk assessments of transgenic crops do not take into consideration whether exogenous proteins interact with endogenous proteins and thereby induce unintended effects in the crops. Therefore, the unintended effects through protein interactions in insect-resistant transgenic rice merit investigation. Here, a yeast two-hybrid assay was used to evaluate interactions between Bacillus thuringiensis (Bt) protein-derived Cry1Ab/c insect resistance rice Huahui-1 and the endogenous proteins of its parental rice Minghui-63. The authenticity of the strongest interactions of Cry1Ab/c and 14 endogenous proteins involved in photosynthesis and stress resistance, which may be primarily responsible for the significant phenotypic differences between transgenic Huahui-1 and parental Minghui-63, were then analyzed and validated by subcellular colocalization, bimolecular fluorescence complementation and co-immunoprecipitation. As the exogenous full-length Cry1Ab/c protein was found to have self-activating activity, we cleaved it-into three segments based on its three domains, and these were screened for interaction with host proteins using the yeast two-hybrid assay. Sixty endogenous proteins related to the regulation of photosynthesis, stress tolerance, and substance metabolism were found to interact with the Cry1Ab/c protein. The results of bimolecular fluorescence complementation and co-immunoprecipitation verified the interactions between the full-length Cry1Ab/c protein and 12 endogenous proteins involved in photosynthesis 23KD, G, PSBP, Rubisco, Trx, THF1 and stress resistance CAMTAs, DAHP, E3s, HKMTs, KIN13A, FREE1. We used a combination of yeast two-hybrid, bimolecular fluorescence complementation, and co-immunoprecipitation to identify Cry1Ab/c interacting with rice proteins that seem to be associated with the observed unintended effects on photosynthesis and stress resistance between Huahui-1 and Minghui-63 rice plants, and analyze the possible interaction mechanisms by comparing differences in cell localization and interaction sites between these interactions. The results herein provide a molecular analytical system to qualify and

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Non-Uniform Distribution Pattern for Differentially Expressed Genes of Transgenic Rice Huahui 1 at Different Developmental Stages and Environments

Cited by 12 publications

References 25 publications

Heterologous expression of heat stress-responsive AtPLC9 confers heat tolerance in transgenic rice

Heterologous expression of heat stress-responsive AtPLC9 confers heat tolerance in transgenic rice

Comparison of three transgenic Bt rice lines for insecticidal protein expression and resistance against a target pest, Chilo suppressalis (Lepidoptera: Crambidae)

Exogenous Cry1Ab/c Protein Recruits Different Endogenous Proteins for Its Function in Plant Growth and Development

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