Non-tuberculous mycobacteria have diverse effects on BCG efficacy against Mycobacterium tuberculosis

Poyntz, Hazel C.; Stylianou, Elena; Griffiths, Kristin; Marsay, Leanne; McShane, Helen

doi:10.1016/j.tube.2013.12.006

Cited by 68 publications

(65 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The BCG vaccine, which is to protect against tuberculosis (TB), offers different levels of protection among adult human populations (18)(19)(20)(21). The variations have been partly attributed to increased exposure to NTM species in some geographical regions (20,21), reducing protection against pulmonary TB in adults (22).…”

mentioning

confidence: 99%

Isolation of Nontuberculous Mycobacteria from the Environment of Ghanian Communities Where Buruli Ulcer Is Endemic

Aboagye

Ampah

Nakobu

et al. 2016

Appl Environ Microbiol

View full text Add to dashboard Cite

This study aimed to isolate nontuberculous mycobacterial species from environmental samples obtained from some selected communities in Ghana. To optimize decontamination, spiked environmental samples were used to evaluate four decontamination solutions and supplemented media, after which the best decontamination solution and media were used for the actual analysis. The isolates obtained were identified on the basis of specific genetic sequences, including heat shock protein 65, IS2404, IS2606, rpoB, and the ketoreductase gene, as needed. Among the methods evaluated, decontamination with 1 M NaOH followed by 5% oxalic acid gave the highest rate of recovery of mycobacteria (50.0%) and the lowest rate of contamination (15.6%). The cultivation medium that supported the highest rate of recovery of mycobacteria was polymyxin B-amphotericin B-nalidixic acidtrimethoprim-azlocillin-supplemented medium (34.4%), followed by isoniazid-supplemented medium (28.1%). Among the 139 samples cultivated in the main analysis, 58 (41.7%) yielded mycobacterial growth, 70 (50.4%) had no growth, and 11 (7.9%) had all inoculated tubes contaminated. A total of 25 different mycobacterial species were identified. Fifteen species (60%) were slowly growing (e.g., Mycobacterium ulcerans, Mycobacterium avium, Mycobacterium mantenii, and Mycobacterium malmoense), and 10 (40%) were rapidly growing (e.g., Mycobacterium chelonae, Mycobacterium fortuitum, and Mycobacterium abscessus). The occurrence of mycobacterial species in the various environmental samples analyzed was as follows: soil, 16 species (43.2%); vegetation, 14 species (38.0%); water, 3 species (8.0%); moss, 2 species (5.4%); snail, 1 species (2.7%); fungi, 1 species (2.7%). This study is the first to report on the isolation of M. ulcerans and other medically relevant nontuberculous mycobacteria from different environmental sources in Ghana. IMPORTANCEDiseases caused by mycobacterial species other than those that cause tuberculosis and leprosy are increasing. Control is difficult because the current understanding of how the organisms are spread and where they live in the environment is limited, although this information is needed to design preventive measures. Growing these organisms from the environment is also difficult, because the culture medium becomes overgrown with other bacteria that also live in the environment, such as in soil and water. We aimed to improve the methods for growing these organisms from environmental sources, such as soil and water samples, for better understanding of important mycobacterial ecology.

show abstract

mentioning

confidence: 99%

Isolation of Nontuberculous Mycobacteria from the Environment of Ghanian Communities Where Buruli Ulcer Is Endemic

Aboagye

Ampah

Nakobu

et al. 2016

Appl Environ Microbiol

View full text Add to dashboard Cite

show abstract

“…91 The findings from several other publications supported this hypothesis, that the increased efficacy of BCG vaccination was observed in locations farther from equator, where the infants suffer from lower exposure to NTMs. 22 This conflicting data provides us several competing factors to keep in mind during development and testing. First, all studies to date were all based on the measurement of immunologic responses.…”

Section: An Appropriate Time For Bcg Vaccinationmentioning

confidence: 99%

“…Several clinical trials have proved that both M. tuberculosis and NTM infection have diverse effects on BCG efficacy against M. tuberculosis. 22,96 Thus, future studies aiming to evaluate the protective effectiveness of revaccination may wish o enroll children clearly documented to have received primary BCG vaccination, and who also have a negative PPD result to prevent the potential interference of M. tuberculosis and NTM infection.…”

Section: Revaccination Of Bcgmentioning

confidence: 99%

“…21 Although the exact reason for these observations is currently unclear, it is hypothesized that prior exposure to NTM may produces antigens which may block the replication of BCG. 22 Further studies are needed to illuminate the effects of NTM exposure on BCG efficacy, which may be helpful in the future, in the development of the candidate vaccines which can be free from the compromise of NTM exposure.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Current status of new tuberculosis vaccine in children

Pang

Zhao

Cohen³

et al. 2016

Human Vaccines & Immunotherapeutics

View full text Add to dashboard Cite

Pediatric tuberculosis contributes significantly to the burden of TB disease worldwide. In order to achieve the goal of eliminating TB by 2050, an effective TB vaccine is urgently needed to prevent TB transmission in children. BCG vaccination can protect children from the severe types of TB such as TB meningitis and miliary TB, while its efficacy against pediatric pulmonary TB ranged from no protection to very high protection. In recent decades, multiple new vaccine candidates have been developed, and shown encouraging safety and immunogenicity in the preclinical experiments. However, the limited data on protective efficacy in infants evaluated by clinical trials has been disappointing, an example being MVA85A. To date, no vaccine has been shown to be clinically safer and more effective than the presently licensed BCG vaccine. Hence, before a new vaccine is developed with more promising efficacy, we must reconsider how to better use the current BCG vaccine to maximize its effectiveness in children.

show abstract

“…Exposure to NTM has been suggested to impact on the efficacy of the Bacille Calmette-Guérin vaccine 16 and to exhibit cross-reactivity to the tuberculin skin test (TST), leading to increased difficulties in interpreting TST-positive results and evaluating the protection through the only available vaccine against TB.…”

Section: -15mentioning

confidence: 99%

Non-tuberculous mycobacteria: baseline data from three sites in Papua New Guinea, 2010–2012

Ley

Carter

Millan

et al. 2015

WPSAR

View full text Add to dashboard Cite

Objective: To determine the proportion of non-tuberculous mycobacteria (NTM) in samples of pulmonary tuberculosis (TB) cases from Papua New Guinea who were diagnosed using acid-fast microscopy. Methods:As part of a case detection study for TB, conducted in three provincial hospitals in Papua New Guinea, sputum samples of suspected tuberculous cases aged 15 years or older were collected from November 2010 to July 2012. Mycobacterial species isolated from sputum and grown in culture were examined to distinguish between NTM and the Mycobacterium tuberculosis complex (MTBC).Results: NTM were detected in 4% (9/225) of sputum samples grown in culture. Five (2.2%) of them were identified as NTM only and four (1.8%) were identified as mixed cultures containing both MTBC and NTM. Four different NTM species were identified; M. fortuitum, M. intracellulare, M. terrae and M. avium.Discussion: This is the first report from Papua New Guinea identifying NTM in three different locations. As NTM cannot be distinguished from M. tuberculosis through smear microscopy, the presence of NTM can lead to a false-positive diagnosis of tuberculosis. The prevalence of NTM should be determined and a diagnostic algorithm developed to confirm acid-fast bacilli in a smear as M. tuberculosis.

show abstract

Non-tuberculous mycobacteria have diverse effects on BCG efficacy against Mycobacterium tuberculosis

Cited by 68 publications

References 50 publications

Isolation of Nontuberculous Mycobacteria from the Environment of Ghanian Communities Where Buruli Ulcer Is Endemic

Isolation of Nontuberculous Mycobacteria from the Environment of Ghanian Communities Where Buruli Ulcer Is Endemic

Current status of new tuberculosis vaccine in children

Non-tuberculous mycobacteria: baseline data from three sites in Papua New Guinea, 2010–2012

Contact Info

Product

Resources

About